FastQCFastQC Report
Fri 10 Feb 2017
ERR1632957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377721
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13120.3473463217560051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.2594507586287233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5180.1371382581323252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT352.3840947E-531.7142878
GGTATCA2450.027.9387741
TTCGCCG809.731593E-1025.437524
GTATCAA7100.024.2323931
GCCGGCA802.7135684E-823.12515
TATACTG652.674933E-622.769235
AGCTTCG903.807145E-922.61111321
CGCCGCT903.807145E-922.61111326
CGATTGG753.7270183E-722.214
TGGTCGA753.7270183E-722.210
CGCTCTC855.1575626E-821.76470629
CTTCGCC957.1067916E-921.42105323
GTCGATT806.934697E-720.812512
TAACGGC806.934697E-720.812536
TTGTCGC450.00382112420.55555737
GCCGCTC909.4360075E-820.55555727
TCGCGCG450.00382112420.5555579
TTTGTCG555.134311E-420.18181836
ACTGTCC555.134311E-420.1818188
GGTCGAT851.2407836E-619.58823411
Produced by FastQC (version 0.11.5)