##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632957.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377721
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.645947670370457	33.0	33.0	33.0	27.0	33.0
2	31.164118489572992	33.0	33.0	33.0	27.0	33.0
3	31.59328181382555	33.0	33.0	33.0	27.0	33.0
4	31.72599617177758	33.0	33.0	33.0	27.0	33.0
5	31.83366294169506	33.0	33.0	33.0	33.0	33.0
6	34.699566611334824	37.0	37.0	37.0	27.0	37.0
7	34.870793522202895	37.0	37.0	37.0	27.0	37.0
8	35.05724066175828	37.0	37.0	37.0	33.0	37.0
9	35.14631434312628	37.0	37.0	37.0	33.0	37.0
10	35.18493808922459	37.0	37.0	37.0	33.0	37.0
11	35.19351849645638	37.0	37.0	37.0	33.0	37.0
12	35.16100772792617	37.0	37.0	37.0	33.0	37.0
13	35.19310549320795	37.0	37.0	37.0	33.0	37.0
14	35.167758742563954	37.0	37.0	37.0	33.0	37.0
15	35.17938901993799	37.0	37.0	37.0	33.0	37.0
16	35.18707988171163	37.0	37.0	37.0	33.0	37.0
17	35.15724304446933	37.0	37.0	37.0	33.0	37.0
18	35.15426995057198	37.0	37.0	37.0	33.0	37.0
19	35.15453999115749	37.0	37.0	37.0	33.0	37.0
20	35.16256443247794	37.0	37.0	37.0	33.0	37.0
21	35.15329039158532	37.0	37.0	37.0	33.0	37.0
22	35.161267178684795	37.0	37.0	37.0	33.0	37.0
23	35.155969617786674	37.0	37.0	37.0	33.0	37.0
24	35.125004963981354	37.0	37.0	37.0	33.0	37.0
25	35.12272815120155	37.0	37.0	37.0	33.0	37.0
26	35.085150150507914	37.0	37.0	37.0	33.0	37.0
27	35.03092494195451	37.0	37.0	37.0	27.0	37.0
28	35.00439477815637	37.0	37.0	37.0	27.0	37.0
29	35.03024189812057	37.0	37.0	37.0	27.0	37.0
30	35.00717725516982	37.0	37.0	37.0	27.0	37.0
31	35.00119929789448	37.0	37.0	37.0	27.0	37.0
32	35.01990357962623	37.0	37.0	37.0	27.0	37.0
33	34.999867627163965	37.0	37.0	37.0	27.0	37.0
34	34.940678437259244	37.0	37.0	37.0	27.0	37.0
35	34.92974973591619	37.0	37.0	37.0	27.0	37.0
36	34.91531844933165	37.0	37.0	37.0	27.0	37.0
37	34.93556355087485	37.0	37.0	37.0	27.0	37.0
38	34.80562108010939	37.0	37.0	37.0	27.0	37.0
39	34.86379629409008	37.0	37.0	37.0	27.0	37.0
40	34.89899423119181	37.0	37.0	37.0	27.0	37.0
41	34.915162249385126	37.0	37.0	37.0	27.0	37.0
42	34.82911990596234	37.0	37.0	37.0	27.0	37.0
43	34.6041337389237	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	23.0
17	22.0
18	18.0
19	14.0
20	32.0
21	98.0
22	266.0
23	575.0
24	1223.0
25	2104.0
26	3445.0
27	5074.0
28	7039.0
29	9537.0
30	12251.0
31	15526.0
32	19276.0
33	25510.0
34	35767.0
35	64881.0
36	175031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.721061312450196	20.52837941231756	13.666171592259897	25.084387682972352
2	15.939542678326065	22.943654178613315	37.83003857344442	23.286764569616196
3	17.790644417440387	26.634738338615012	30.456342114947276	25.11827512899733
4	12.831693233894859	17.991851128213682	38.94779480092449	30.22866083696697
5	14.15515684857342	36.64238948853784	35.355990267949096	13.84646339493965
6	29.68407898951872	39.810336200528965	17.31595542741865	13.189629382533669
7	26.55028446922464	32.40804720944824	23.41543096624228	17.626237355084836
8	24.422788248469107	36.0453879980197	20.48734383314669	19.044479920364502
9	25.94322264316784	15.501653336722077	20.3020218626976	38.253102157412485
10	14.85196745746199	28.133728333876064	34.00949377980044	23.004810428861514
11	34.138689667770656	23.440581805088943	24.244084919821773	18.176643607318628
12	23.301590327252125	25.524394989952903	30.16326865596565	21.010746026829327
13	27.94708263506662	22.26061034467239	26.128544613616928	23.663762406644057
14	21.92279486711091	20.944030117467655	27.722048813807014	29.41112620161442
15	24.06564633684651	28.3664397796257	24.441055699841947	23.12685818368584
16	23.135859536536227	28.303165564000942	25.2027289983877	23.35824590107513
17	22.502587888944486	27.313810987474884	26.588407846002738	23.595193277577895
18	23.301060835907986	27.004588042496973	27.514223461231968	22.180127660363073
19	24.880268769806285	26.311483873017387	27.010412447282516	21.79783490989381
20	24.10959411841015	26.31810251481914	27.279129304433695	22.293174062337016
21	23.342625906422995	26.759698295832106	27.449096025902715	22.44857977184218
22	22.979659590014855	26.737989150722356	27.20394153356578	23.07840972569701
23	22.840932857850106	26.814500649950624	27.719136611414246	22.625429880785024
24	23.296824905154864	27.188851030257783	26.309895398984967	23.20442866560239
25	23.406429613391897	26.725281358462993	27.29183709669306	22.576451931452052
26	23.234344926546314	26.95163890808295	27.292366588037204	22.521649577333534
27	23.27114457496406	26.68053933988314	26.838063014764867	23.21025307038793
28	22.820812186772777	26.506071941988928	27.613767833930336	23.059348037307963
29	23.786869144156668	26.649299350578865	26.681333576899352	22.882497928365115
30	23.524770928807243	26.414734685124735	27.273834390992292	22.78665999507573
31	23.405900122047754	26.523545156345556	27.057801922583067	23.012752799023616
32	22.289997114272175	26.57384683403888	27.535667860669648	23.6004881910193
33	22.496763484158944	26.62441325740427	28.02650633668766	22.85231692174912
34	23.190397144982672	26.35463741756482	27.43374077692265	23.021224660529864
35	23.08238091077806	27.052771754813737	27.193881198027114	22.670966136381086
36	23.90018029180268	26.539429896669763	26.762345752552807	22.798044058974746
37	23.18351375750885	26.241855761262944	27.070244969170364	23.50438551205784
38	23.233021198185963	26.802057603363327	26.854212500761143	23.110708697689564
39	22.95212604011956	26.642151217432975	27.232269320477283	23.173453421970184
40	22.923268761863916	27.181438151439817	27.400647567913882	22.494645518782384
41	23.12500496398135	26.10392326611441	27.402765533290445	23.368306236613797
42	22.622517678392253	26.980760932010668	27.33075471048737	23.065966679109714
43	23.321975744001524	25.95275348736237	27.47239364504489	23.252877123591222
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	9.0
2	15.0
3	63.5
4	112.0
5	112.0
6	158.5
7	205.0
8	201.0
9	197.0
10	245.0
11	293.0
12	293.0
13	477.0
14	661.0
15	1209.5
16	1758.0
17	2015.5
18	2273.0
19	2273.0
20	2148.5
21	2024.0
22	2353.0
23	2682.0
24	3737.5
25	4793.0
26	4793.0
27	6231.0
28	7669.0
29	9161.5
30	10654.0
31	12728.5
32	14803.0
33	14803.0
34	17132.0
35	19461.0
36	21474.5
37	23488.0
38	25291.5
39	27095.0
40	27095.0
41	28247.0
42	29399.0
43	30251.0
44	31103.0
45	30632.5
46	30162.0
47	30162.0
48	29140.0
49	28118.0
50	28049.5
51	27981.0
52	26688.0
53	25395.0
54	25395.0
55	23803.0
56	22211.0
57	19726.5
58	17242.0
59	15932.5
60	14623.0
61	14623.0
62	12895.5
63	11168.0
64	9353.0
65	7538.0
66	6445.5
67	5353.0
68	5353.0
69	4568.0
70	3783.0
71	3081.5
72	2380.0
73	1898.5
74	1417.0
75	1417.0
76	1037.0
77	657.0
78	543.0
79	429.0
80	332.0
81	235.0
82	235.0
83	189.5
84	144.0
85	127.5
86	111.0
87	82.5
88	54.0
89	54.0
90	39.0
91	24.0
92	15.0
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	377721.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.8745038254081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.19360625706881	58.97903659014884
2	6.576052452683253	8.795404898061012
3	1.9365201282047713	3.885114681648294
4	0.8719952005146809	2.3325698549022613
5	0.5057504316344116	1.691090458751863
6	0.3472629423284128	1.3933822179098352
7	0.23459313522094075	1.0981809663123097
8	0.17240055697944065	0.9223361365779275
9	0.1259015514193003	0.7577643403813331
>10	0.9175467978190186	11.80696316680266
>50	0.08289923769213642	3.766883022622717
>100	0.034275646352717175	3.8223214764386135
>500	7.97108054714353E-4	0.399263480350397
>1k	3.985540273571765E-4	0.34968870909193644
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1312	0.3473463217560051	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	980	0.2594507586287233	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	518	0.1371382581323252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.6474567207012583E-4	0.0	0.0
4	0.0	0.0	5.294913441402517E-4	2.6474567207012583E-4	0.0
5	0.0	0.0	0.0010589826882805033	2.6474567207012583E-4	0.0
6	0.0	0.0	0.0010589826882805033	2.6474567207012583E-4	0.0
7	0.0	0.0	0.002912202392771384	2.6474567207012583E-4	0.0
8	0.0	0.0	0.004235930753122013	2.6474567207012583E-4	0.0
9	0.0	0.0	0.005294913441402517	5.294913441402517E-4	0.0
10	2.6474567207012583E-4	0.0	0.006883387473823272	5.294913441402517E-4	0.0
11	2.6474567207012583E-4	0.0	0.009001352850384278	5.294913441402517E-4	0.0
12	2.6474567207012583E-4	0.0	0.010060335538664781	5.294913441402517E-4	0.0
13	2.6474567207012583E-4	0.0	0.01085457255487516	5.294913441402517E-4	0.0
14	2.6474567207012583E-4	0.0	0.012972537931436165	5.294913441402517E-4	0.0
15	2.6474567207012583E-4	0.0	0.015090503307997173	5.294913441402517E-4	0.0
16	2.6474567207012583E-4	0.0	0.016678977340417928	5.294913441402517E-4	0.0
17	2.6474567207012583E-4	0.0	0.017473214356628304	7.942370162103775E-4	0.0
18	2.6474567207012583E-4	0.0	0.018002705700768555	7.942370162103775E-4	0.0
19	2.6474567207012583E-4	0.0	0.018267451372838684	7.942370162103775E-4	0.0
20	2.6474567207012583E-4	0.0	0.018796942716978935	7.942370162103775E-4	0.0
21	2.6474567207012583E-4	0.0	0.019326434061119186	7.942370162103775E-4	0.0
22	2.6474567207012583E-4	0.0	0.019326434061119186	0.0010589826882805033	0.0
23	2.6474567207012583E-4	0.0	0.01959117973318931	0.0010589826882805033	0.0
24	2.6474567207012583E-4	0.0	0.019855925405259437	0.001588474032420755	0.0
25	2.6474567207012583E-4	0.0	0.019855925405259437	0.001588474032420755	0.0
26	2.6474567207012583E-4	0.0	0.019855925405259437	0.001588474032420755	0.0
27	2.6474567207012583E-4	0.0	0.019855925405259437	0.003441693736911636	0.0
28	2.6474567207012583E-4	0.0	0.019855925405259437	0.009795589866594656	0.0
29	2.6474567207012583E-4	0.0	0.019855925405259437	0.024356601830451578	0.0
30	2.6474567207012583E-4	0.0	0.019855925405259437	0.04580100126813177	0.0
31	2.6474567207012583E-4	0.0	0.019855925405259437	0.1085457255487516	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCAT	35	2.3840947E-5	31.714287	8
GGTATCA	245	0.0	27.938774	1
TTCGCCG	80	9.731593E-10	25.4375	24
GTATCAA	710	0.0	24.232393	1
GCCGGCA	80	2.7135684E-8	23.125	15
TATACTG	65	2.674933E-6	22.76923	5
AGCTTCG	90	3.807145E-9	22.611113	21
CGCCGCT	90	3.807145E-9	22.611113	26
CGATTGG	75	3.7270183E-7	22.2	14
TGGTCGA	75	3.7270183E-7	22.2	10
CGCTCTC	85	5.1575626E-8	21.764706	29
CTTCGCC	95	7.1067916E-9	21.421053	23
GTCGATT	80	6.934697E-7	20.8125	12
TAACGGC	80	6.934697E-7	20.8125	36
TTGTCGC	45	0.003821124	20.555557	37
GCCGCTC	90	9.4360075E-8	20.555557	27
TCGCGCG	45	0.003821124	20.555557	9
TTTGTCG	55	5.134311E-4	20.181818	36
ACTGTCC	55	5.134311E-4	20.181818	8
GGTCGAT	85	1.2407836E-6	19.588234	11
>>END_MODULE