FastQCFastQC Report
Fri 10 Feb 2017
ERR1632956.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632956.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274655
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95873.4905608854745043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75762.758369590941363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39791.4487265842602537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.688136025195245No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18230.663741785148641No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG12550.45693688445504366No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.4110611494420273No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10180.37064681145437No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9840.3582676448635561No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9570.34843713021790973No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7980.29054632174910344No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7780.28326445904862463No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7300.26578798856747554No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6560.23884509657570407No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.22828639566000983No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6120.22282499863465074No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6020.21918406728441134No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5910.215179042799148No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.20207168993828623No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5450.19843075858804682No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5390.19624619977790317No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5310.19333345469771168No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5280.19224117529263984No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5250.19114889588756806No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5250.19114889588756806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.18750796453732865No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5080.18495931259216106No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4890.17804154302670622No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4860.1769492636216344No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4840.17622107735158654No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4820.17549289108153868No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4770.17367242540641897No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4660.16966740092115562No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4580.16675465584096413No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4500.1638419107607726No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4440.16165735195062897No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4410.16056507254555716No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4350.1583805137354135No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4240.15437548925015018No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4210.15328320984507837No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4190.1525550235750305No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4130.15037046476488686No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4080.14854999908976715No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4070.1481859059547432No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG4070.1481859059547432No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG4060.1478218128197193No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4040.1470936265496714No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3980.14490906773952777No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3950.14381678833445596No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC3950.14381678833445596No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.1398117638491926No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3820.13908357757914475No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3810.13871948444412083No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3760.13689901876900112No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3750.13653492563397718No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3740.13617083249895323No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3650.13289399428373777No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3610.13143762174364204No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3590.13070943547359415No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3580.1303453423385702No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3510.12779669039340263No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG3430.1248839453132111No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3360.12233529336804355No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3350.1219712002330196No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3340.12160710709799567No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.12124301396297173No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3280.11942254828785204No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3270.11905845515282809No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3170.11541752380258871No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3150.11468933753254083No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3080.11214068558737325No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3080.11214068558737325No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3010.10959203364220567No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2980.10849975423713387No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2950.10740747483206205No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA2940.10704338169703811No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.10558700915694234No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC2860.10413063661684659No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2830.10303835721177476No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2800.10194607780670295No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2790.101581984671679No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2780.10121789153665509No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA2780.10121789153665509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA401.5942042E-632.3751
ATTTAGT250.00548915329.62
TCCGACT250.00548915329.631
CGGCAGT250.00548915329.618
GGTATCA13450.027.646841
GATTCCG358.852147E-426.4285728
GTATTAT659.337418E-825.6153831
TATTATA659.337418E-825.6153832
ATTATAG659.337418E-825.6153833
CTAGTGC601.3312238E-624.6666669
ACTAGTG1450.024.2413798
TTATAGA701.9118488E-723.7857134
CTAGTGG701.9118488E-723.7857139
TATAGAT701.9118488E-723.7857135
ATACTAG2500.023.6800026
CATACTA2550.023.2156875
GGATGGG400.001927467623.12519
ATAGATC802.7066562E-823.1256
AGTGCAA652.670522E-622.76922811
TAGTGCA652.670522E-622.76922810
Produced by FastQC (version 0.11.5)