FastQCFastQC Report
Fri 10 Feb 2017
ERR1632955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325887
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83502.5622378309045772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65622.0135813947779444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34571.0607971474774998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.4918882925676692No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG14340.44002982628948073No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9980.3062411203883555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.2847612822849638No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9140.2804653146642855No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8760.26880483112244424No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8430.2586786217308454No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7190.22062862280483728No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6470.19853507504134868No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6140.18840886564974976No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5950.18257862387882917No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5700.17490725312761787No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.16386047924587357No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5180.15895080196509834No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5110.15680281815475916No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4790.14698346359320869No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4690.14391491529272415No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4680.1436080604626757No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4640.1423806411424819No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4520.13869838318190048No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4500.1380846735218036No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4390.1347092703912706No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.12857217379030156No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.12765160930015618No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4100.1258104803198655No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4060.12458306099967166No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4040.12396935133957476No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG4000.12274193201938097No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3950.1212076578691387No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3950.1212076578691387No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3940.12090080303909023No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA3820.11721854507850882No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3760.1153774160982181No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3620.11108144847753977No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3570.1095471743272975No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3550.10893346466720058No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3540.10862660983715214No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3530.10831975500710368No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3510.10770604534700678No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3510.10770604534700678No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3500.10739919051695831No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3500.10739919051695831No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG3490.10709233568690989No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3470.10647862602681299No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3470.10647862602681299No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3440.10555806153666762No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3440.10555806153666762No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG3420.10494435187657072No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.10463749704652225No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3410.10463749704652225No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3400.1043306422164738No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3400.1043306422164738No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3380.1037169325563769No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3380.1037169325563769No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3380.1037169325563769No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3370.10341007772632846No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3350.10279636806623153No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC3350.10279636806623153No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3340.10248951323618309No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3310.10156894874603774No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3280.10064838425589237No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3270.10034152942584393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTG705.456968E-1231.7142858
CTAGTGG352.3830979E-531.7142859
CTAGTGC303.5938693E-430.8333349
TACCCAT250.00549063629.67
ATAATCG250.00549063629.615
GGCAACT250.00549063629.630
CTGACTA250.00549063629.635
AATGAAA250.00549063629.62
TTTGGCA250.00549063629.627
ATACCCA250.00549063629.66
GACCGTT453.996678E-628.7777797
GGTATCA11750.027.080851
TGATTGT358.855708E-426.4285722
TCCCGAA358.855708E-426.4285719
TCATAAT358.855708E-426.4285713
ATCATAA358.855708E-426.4285712
GCTCTCT3850.024.02597230
CGCTCTC3850.024.02597229
ATACTAG1400.023.7857136
CCGCTCT3900.023.71794928
Produced by FastQC (version 0.11.5)