##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632955.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325887
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.577298265963357	33.0	33.0	33.0	27.0	33.0
2	31.08304719120431	33.0	33.0	33.0	27.0	33.0
3	31.52178209011099	33.0	33.0	33.0	27.0	33.0
4	31.64845483250329	33.0	33.0	33.0	27.0	33.0
5	31.791507485723578	33.0	33.0	33.0	33.0	33.0
6	34.54831889581358	37.0	33.0	37.0	27.0	37.0
7	34.755399877871774	37.0	37.0	37.0	27.0	37.0
8	34.961977618008696	37.0	37.0	37.0	27.0	37.0
9	35.05095938162615	37.0	37.0	37.0	27.0	37.0
10	35.00468567325484	37.0	37.0	37.0	27.0	37.0
11	35.04134868834903	37.0	37.0	37.0	27.0	37.0
12	34.974126000730315	37.0	37.0	37.0	27.0	37.0
13	35.01283266899263	37.0	37.0	37.0	27.0	37.0
14	34.97413213782691	37.0	37.0	37.0	27.0	37.0
15	35.033039059551314	37.0	37.0	37.0	27.0	37.0
16	35.05639684921461	37.0	37.0	37.0	27.0	37.0
17	35.04590548257525	37.0	37.0	37.0	27.0	37.0
18	35.04720654705466	37.0	37.0	37.0	27.0	37.0
19	35.01553912859365	37.0	37.0	37.0	27.0	37.0
20	35.01705806000239	37.0	37.0	37.0	27.0	37.0
21	35.02010512846478	37.0	37.0	37.0	27.0	37.0
22	35.02178362438514	37.0	37.0	37.0	27.0	37.0
23	35.0227747654862	37.0	37.0	37.0	27.0	37.0
24	35.00493422566718	37.0	37.0	37.0	27.0	37.0
25	34.98390546416396	37.0	37.0	37.0	27.0	37.0
26	34.945683626533125	37.0	37.0	37.0	27.0	37.0
27	34.886911720933945	37.0	37.0	37.0	27.0	37.0
28	34.81603439228935	37.0	37.0	37.0	27.0	37.0
29	34.84130695609214	37.0	37.0	37.0	27.0	37.0
30	34.787441659225436	37.0	37.0	37.0	27.0	37.0
31	34.73459818894279	37.0	37.0	37.0	27.0	37.0
32	34.721492419151424	37.0	37.0	37.0	27.0	37.0
33	34.67896540825501	37.0	37.0	37.0	27.0	37.0
34	34.5775713667621	37.0	37.0	37.0	27.0	37.0
35	34.51925667485969	37.0	37.0	37.0	27.0	37.0
36	34.44110074964635	37.0	37.0	37.0	27.0	37.0
37	34.41258473028995	37.0	37.0	37.0	27.0	37.0
38	34.20973834488642	37.0	37.0	37.0	27.0	37.0
39	34.21082461098479	37.0	37.0	37.0	27.0	37.0
40	34.15993580596955	37.0	37.0	37.0	27.0	37.0
41	34.065062429615175	37.0	37.0	37.0	27.0	37.0
42	33.85446489120462	37.0	37.0	37.0	27.0	37.0
43	33.54625069425905	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	30.0
17	22.0
18	25.0
19	30.0
20	67.0
21	104.0
22	293.0
23	690.0
24	1318.0
25	2204.0
26	3683.0
27	5297.0
28	7282.0
29	9673.0
30	12530.0
31	15543.0
32	18845.0
33	23982.0
34	32339.0
35	55064.0
36	136856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.71707370959873	17.943029332253204	15.064117316738624	21.275779641409446
2	16.2482701058956	24.65977470718378	38.60018963628497	20.491765550635648
3	18.31800593457241	29.787625772123466	30.798098727473022	21.0962695658311
4	11.340740808930704	18.384900287522974	40.22866821935211	30.045690684194216
5	14.213515727844314	36.202425994286365	32.124939012602525	17.459119265266796
6	25.067277921488124	42.82343266224182	15.802103183005153	16.307186233264908
7	24.41950737525584	34.55768410522666	21.33684375258909	19.685964766928414
8	25.340378720231243	32.559752306781185	19.13884260495202	22.961026368035544
9	29.103646355945468	12.620018595402701	18.763866002632813	39.512469046019014
10	17.46188095873723	26.590198443018593	29.99137737927564	25.956543218968537
11	34.807770791716145	24.80829244492723	20.938239328356147	19.445697435000476
12	25.35357347792333	27.839097601315792	26.372024658854144	20.435304261906733
13	31.312694277464274	23.188712651931496	24.87764163651818	20.62095143408605
14	24.23692875137701	21.46909818433997	30.077910441349303	24.216062622933716
15	26.66967384400114	25.88105693077662	27.992525016340018	19.45674420888222
16	20.011230886779774	28.509268550141613	26.59664239444961	24.882858168629003
17	21.849291318770003	24.706416641351144	25.804036368434456	27.640255671444397
18	25.367381945275508	21.778100998198763	29.903310043051732	22.951207013473997
19	27.911822196037278	22.499823558472723	30.61797494223458	18.97037930325542
20	26.389515384166906	21.63970946984691	31.032535817630034	20.938239328356147
21	22.34547557895835	25.169767434724307	31.205908796607414	21.278848189709933
22	22.725668713388384	24.67695857766649	31.160801136590287	21.436571572354836
23	21.912810268590032	24.71869083455308	32.373184570111725	20.99531432674516
24	22.25802195239454	24.769628736341122	31.29949951977219	21.672849791492144
25	21.924470752131874	24.946377118449035	33.33762930095401	19.79152282846508
26	22.154611874668213	25.227456142773413	31.445562418875255	21.172369563683116
27	22.77231064755575	24.444055761659715	32.007413612693966	20.776219978090566
28	21.17819980545404	24.83959163759217	31.746280152322736	22.235928404631053
29	22.50227839711311	25.163630338123337	30.28718543544235	22.046905829321204
30	22.9107021759076	24.367648908977653	32.083206755715935	20.63844215939881
31	21.761223982546095	24.849104137323675	31.571679754025165	21.81799212610506
32	19.776180086962658	24.83928478276212	32.225587396858415	23.1589477334168
33	20.230938945094465	24.906485990542734	32.67206117457892	22.190513889783883
34	20.898961910109946	24.91783961925453	32.25197691224258	21.93122155839294
35	20.664524819952927	26.221358937300355	31.53976685170013	21.574349391046592
36	21.370597783894418	25.731004918882928	31.76008861967492	21.13830867754774
37	21.753245756964837	25.8347218514393	31.201612828986736	21.210419562609125
38	21.26902883514838	25.87154443104512	30.04507697453412	22.814349759272385
39	20.65225062675099	25.149821870771156	30.942320497595794	23.25560700488206
40	20.633839336948085	26.963333916357513	31.459370886227433	20.943455860466972
41	21.71918487082946	25.389475493038997	30.366967691254942	22.5243719448766
42	20.282490556542605	27.056310929862192	30.769868083108566	21.89133043048664
43	21.2951114957025	26.06885208676627	30.300993902794527	22.335042514736703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	48.0
2	92.0
3	399.5
4	707.0
5	707.0
6	937.0
7	1167.0
8	1215.5
9	1264.0
10	1687.0
11	2110.0
12	2110.0
13	3328.0
14	4546.0
15	7882.0
16	11218.0
17	11650.5
18	12083.0
19	12083.0
20	9281.5
21	6480.0
22	4463.5
23	2447.0
24	2221.5
25	1996.0
26	1996.0
27	2094.5
28	2193.0
29	2637.0
30	3081.0
31	4134.0
32	5187.0
33	5187.0
34	7137.5
35	9088.0
36	10014.0
37	10940.0
38	12483.5
39	14027.0
40	14027.0
41	16264.0
42	18501.0
43	20519.0
44	22537.0
45	25134.0
46	27731.0
47	27731.0
48	29217.5
49	30704.0
50	32583.5
51	34463.0
52	32383.0
53	30303.0
54	30303.0
55	26803.5
56	23304.0
57	19747.0
58	16190.0
59	14695.5
60	13201.0
61	13201.0
62	10431.5
63	7662.0
64	5654.5
65	3647.0
66	3725.0
67	3803.0
68	3803.0
69	3108.0
70	2413.0
71	1860.0
72	1307.0
73	1263.5
74	1220.0
75	1220.0
76	714.5
77	209.0
78	122.0
79	35.0
80	23.0
81	11.0
82	11.0
83	10.0
84	9.0
85	5.5
86	2.0
87	3.0
88	4.0
89	4.0
90	2.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	325887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.234019767588155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30174653030616	21.543050198381646
2	9.132603710425927	5.157002273794291
3	3.754985816913195	3.180550313452209
4	2.080186064709654	2.3492805788509514
5	1.4585212637619416	2.0589959096251156
6	0.9357576811468195	1.5852120520303052
7	0.7596917759833064	1.5014406834270775
8	0.6292725869732967	1.4213515727844315
9	0.43038332373303195	1.0936306142926842
>10	3.644129506254687	21.271790528618816
>50	0.4945060916629534	9.796033594466794
>100	0.3586527697775266	19.6295034781995
>500	0.014128745476084382	2.843623710059008
>1k	0.003260479725250242	1.9927152663346497
>5k	0.002173653150166828	4.575819225682522
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8350	2.5622378309045772	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6562	2.0135813947779444	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3457	1.0607971474774998	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1603	0.4918882925676692	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1434	0.44002982628948073	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	998	0.3062411203883555	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	928	0.2847612822849638	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	914	0.2804653146642855	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	876	0.26880483112244424	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	843	0.2586786217308454	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	719	0.22062862280483728	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	647	0.19853507504134868	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	614	0.18840886564974976	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	595	0.18257862387882917	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	570	0.17490725312761787	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	534	0.16386047924587357	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	518	0.15895080196509834	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	511	0.15680281815475916	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	479	0.14698346359320869	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	469	0.14391491529272415	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	468	0.1436080604626757	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	464	0.1423806411424819	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	452	0.13869838318190048	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	450	0.1380846735218036	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	439	0.1347092703912706	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	419	0.12857217379030156	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	416	0.12765160930015618	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	410	0.1258104803198655	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	406	0.12458306099967166	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	404	0.12396935133957476	No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG	400	0.12274193201938097	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	395	0.1212076578691387	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	395	0.1212076578691387	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	394	0.12090080303909023	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	382	0.11721854507850882	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	376	0.1153774160982181	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	362	0.11108144847753977	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	357	0.1095471743272975	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	355	0.10893346466720058	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	354	0.10862660983715214	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	353	0.10831975500710368	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	351	0.10770604534700678	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	351	0.10770604534700678	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	350	0.10739919051695831	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	350	0.10739919051695831	No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG	349	0.10709233568690989	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	347	0.10647862602681299	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	347	0.10647862602681299	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	344	0.10555806153666762	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	344	0.10555806153666762	No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG	342	0.10494435187657072	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	341	0.10463749704652225	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	341	0.10463749704652225	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	340	0.1043306422164738	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	340	0.1043306422164738	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	338	0.1037169325563769	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	338	0.1037169325563769	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	338	0.1037169325563769	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	337	0.10341007772632846	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	335	0.10279636806623153	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	335	0.10279636806623153	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	334	0.10248951323618309	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	331	0.10156894874603774	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	328	0.10064838425589237	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	327	0.10034152942584393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0024548386403876192	0.0	0.0
2	3.068548300484524E-4	0.0	0.003068548300484524	0.0	0.0
3	3.068548300484524E-4	0.0	0.005216532110823691	0.0	0.0
4	3.068548300484524E-4	0.0	0.009512499731502025	0.0	0.0
5	6.137096600969048E-4	0.0	0.015956451162519522	3.068548300484524E-4	0.0
6	6.137096600969048E-4	0.0	0.02823064436445762	6.137096600969048E-4	0.0
7	9.205644901453572E-4	0.0	0.06382580465007809	6.137096600969048E-4	0.0
8	9.205644901453572E-4	0.0	0.07395201404167702	6.137096600969048E-4	0.0
9	0.0012274193201938096	0.0	0.08745362656380892	6.137096600969048E-4	0.0
10	0.004602822450726786	0.0	0.10064838425589238	9.205644901453572E-4	0.0
11	0.004602822450726786	0.0	0.14452862495282107	9.205644901453572E-4	0.0
12	0.004602822450726786	0.0	0.16017822128529213	0.0012274193201938096	0.0
13	0.0049096772807752384	0.0	0.17674838210790858	0.0012274193201938096	0.0
14	0.005216532110823691	0.0	0.19178426878028274	0.0012274193201938096	0.0
15	0.005216532110823691	0.0	0.215718945524062	0.0012274193201938096	0.0
16	0.005523386940872143	0.0	0.2344370901570176	0.0012274193201938096	0.0
17	0.005523386940872143	0.0	0.23873305777769596	0.0021479838103391664	0.0
18	0.006137096600969048	0.0	0.24640442852890726	0.0021479838103391664	0.0
19	0.006137096600969048	0.0	0.2513141058096825	0.0021479838103391664	0.0
20	0.006137096600969048	0.0	0.25745120241065156	0.0021479838103391664	0.0
21	0.006137096600969048	0.0	0.2654294279919113	0.0027616934704360716	0.0
22	0.006137096600969048	0.0	0.26911168595249274	0.0039891127906298805	0.0
23	0.006137096600969048	0.0	0.27095281493278345	0.005523386940872143	0.0
24	0.006137096600969048	0.0	0.27218023425297727	0.009205644901453572	0.0
25	0.006137096600969048	0.0	0.2731007987431226	0.009819354561550477	0.0
26	0.006137096600969048	0.0	0.27371450840321954	0.012274193201938096	0.0
27	0.006137096600969048	0.0	0.274021363233268	0.019638709123100954	0.0
28	0.006137096600969048	0.0	0.274021363233268	0.054927014578672974	0.0
29	0.006137096600969048	0.0	0.274021363233268	0.14207378631243345	0.0
30	0.006137096600969048	0.0	0.2743282180633164	0.23873305777769596	0.0
31	0.006137096600969048	0.0	0.2743282180633164	0.4323584555382694	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGTG	70	5.456968E-12	31.714285	8
CTAGTGG	35	2.3830979E-5	31.714285	9
CTAGTGC	30	3.5938693E-4	30.833334	9
TACCCAT	25	0.005490636	29.6	7
ATAATCG	25	0.005490636	29.6	15
GGCAACT	25	0.005490636	29.6	30
CTGACTA	25	0.005490636	29.6	35
AATGAAA	25	0.005490636	29.6	2
TTTGGCA	25	0.005490636	29.6	27
ATACCCA	25	0.005490636	29.6	6
GACCGTT	45	3.996678E-6	28.777779	7
GGTATCA	1175	0.0	27.08085	1
TGATTGT	35	8.855708E-4	26.42857	22
TCCCGAA	35	8.855708E-4	26.42857	19
TCATAAT	35	8.855708E-4	26.42857	13
ATCATAA	35	8.855708E-4	26.42857	12
GCTCTCT	385	0.0	24.025972	30
CGCTCTC	385	0.0	24.025972	29
ATACTAG	140	0.0	23.785713	6
CCGCTCT	390	0.0	23.717949	28
>>END_MODULE