FastQCFastQC Report
Fri 10 Feb 2017
ERR1632954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466414
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16430.35226215336589384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.2598549786241408No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8290.17773909016453193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6990.14986685648372475No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6900.14793724030582273No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6800.145793222330376No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6770.145150016937742No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5860.125639453361177No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5640.12092261381519423No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5470.11727778325693482No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5470.11727778325693482No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4980.10677209517724598No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4850.10398487180916524No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4730.10141205023862919No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4730.10141205023862919No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4700.1007688448459952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGC250.00549303729.610
GGTATCA2650.025.8301891
GTATCAA8600.024.9534871
TGCGGTA451.321566E-424.66666636
GCCGGCA952.8012437E-1023.36842215
CGCTCTC1054.0017767E-1122.9047629
GTATTAG1300.022.769231
CGGCCTT1155.456968E-1222.5217424
TCGCCAT1301.8189894E-1221.34615313
TCTCGCC1151.3460522E-1020.91304412
GTATAAC450.003822451220.5555551
AGCTTCG1001.2849341E-820.3521
ATTGCCG555.1368E-420.18181811
CGCTACA656.890898E-519.9230772
TGCTCGC1303.274181E-1119.92307710
TAAGGGT759.246425E-619.7333344
CTGCTCG2000.019.4250019
GCTTCGC1153.0358933E-919.30434822
CCGCTCT1254.110916E-1019.2428
CTCGCCT1254.110916E-1019.2413
Produced by FastQC (version 0.11.5)