FastQCFastQC Report
Fri 10 Feb 2017
ERR1632953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438326
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22420.511491447005197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.35795275662406517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8500.19391959409206846No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5080.11589547505737739No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4410.10061004822894376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3200.027.1718771
TCTACGC509.0743415E-625.90000210
GTATCAA9550.023.6335071
GGCTACG551.8991785E-523.5454541
AATGACG400.001929291423.12500219
CAAGGCG400.001929291423.1250029
CGAGCGT502.6984073E-422.26
ACGGGAT855.162292E-821.76470616
GCTACGA705.090047E-621.1428592
CGCGGAA656.8896836E-519.92307733
ATTCGCG656.8896836E-519.92307730
GCGAACG902.14604E-618.522
CGGCCAA701.2170144E-418.524
GATTTAG609.2243904E-418.51
TACGAGC609.2243904E-418.54
ATTGACG801.6129543E-518.517
TCGCGGA701.2170144E-418.532
CGAACCG1351.1459633E-917.81481631
GCGAACC1351.1459633E-917.81481630
CCGTATT953.5960966E-617.5263161
Produced by FastQC (version 0.11.5)