##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632953.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438326
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68188973503739	33.0	33.0	33.0	27.0	33.0
2	31.188565131888137	33.0	33.0	33.0	27.0	33.0
3	31.61484830924928	33.0	33.0	33.0	27.0	33.0
4	31.75413505016814	33.0	33.0	33.0	27.0	33.0
5	31.857781651099867	33.0	33.0	33.0	33.0	33.0
6	34.70460114161606	37.0	37.0	37.0	27.0	37.0
7	34.906729238055696	37.0	37.0	37.0	27.0	37.0
8	35.08172227976438	37.0	37.0	37.0	33.0	37.0
9	35.177721604467905	37.0	37.0	37.0	33.0	37.0
10	35.21329330224536	37.0	37.0	37.0	33.0	37.0
11	35.23250959331639	37.0	37.0	37.0	33.0	37.0
12	35.193550006159796	37.0	37.0	37.0	33.0	37.0
13	35.21194727212166	37.0	37.0	37.0	33.0	37.0
14	35.1884442173177	37.0	37.0	37.0	33.0	37.0
15	35.20196839795038	37.0	37.0	37.0	33.0	37.0
16	35.21933218654609	37.0	37.0	37.0	33.0	37.0
17	35.17753681050177	37.0	37.0	37.0	33.0	37.0
18	35.17863644867062	37.0	37.0	37.0	33.0	37.0
19	35.19409754383724	37.0	37.0	37.0	33.0	37.0
20	35.19810597591747	37.0	37.0	37.0	33.0	37.0
21	35.18768450879026	37.0	37.0	37.0	33.0	37.0
22	35.202874116525145	37.0	37.0	37.0	33.0	37.0
23	35.171589182480616	37.0	37.0	37.0	33.0	37.0
24	35.17908360444053	37.0	37.0	37.0	33.0	37.0
25	35.16965454935368	37.0	37.0	37.0	33.0	37.0
26	35.14236664035444	37.0	37.0	37.0	33.0	37.0
27	35.079007405447086	37.0	37.0	37.0	33.0	37.0
28	35.05409444112373	37.0	37.0	37.0	27.0	37.0
29	35.0946601388008	37.0	37.0	37.0	33.0	37.0
30	35.04694451161921	37.0	37.0	37.0	27.0	37.0
31	35.04843655179022	37.0	37.0	37.0	27.0	37.0
32	35.076274279873886	37.0	37.0	37.0	33.0	37.0
33	35.042349757942716	37.0	37.0	37.0	27.0	37.0
34	34.976318995450875	37.0	37.0	37.0	27.0	37.0
35	34.96509903587741	37.0	37.0	37.0	27.0	37.0
36	34.92622386077942	37.0	37.0	37.0	27.0	37.0
37	34.9646952268403	37.0	37.0	37.0	27.0	37.0
38	34.810551963607	37.0	37.0	37.0	27.0	37.0
39	34.88937229368096	37.0	37.0	37.0	27.0	37.0
40	34.915156755474236	37.0	37.0	37.0	27.0	37.0
41	34.92083517747065	37.0	37.0	37.0	27.0	37.0
42	34.830619219485044	37.0	37.0	37.0	27.0	37.0
43	34.599533224129985	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	16.0
16	18.0
17	27.0
18	13.0
19	18.0
20	34.0
21	101.0
22	249.0
23	631.0
24	1311.0
25	2303.0
26	3752.0
27	5657.0
28	8196.0
29	10957.0
30	14021.0
31	17705.0
32	22462.0
33	29464.0
34	41376.0
35	74484.0
36	205529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56061926511318	19.375533278883754	13.479008774291282	25.584838681711787
2	16.40194740900608	22.700227684417534	37.2902816625069	23.607543244069483
3	18.458407669177735	25.899900986936665	29.788559200229965	25.853132143655632
4	13.238776618316047	17.75048708039222	37.917896725268406	31.092839576023323
5	15.130063012461045	35.637402298745684	34.00368675369474	15.228847935098532
6	30.299822506536234	38.47273490507065	17.32203884779821	13.905403740594899
7	27.449660754780687	31.791634536851564	22.32470809397572	18.43399661439203
8	25.146808539762645	34.78780633592349	20.34672823423662	19.71865689007725
9	26.97124970912061	14.954850955681387	19.348612676409797	38.725286658788214
10	15.309609742520408	27.68989291075592	32.97477220151212	24.025725145211556
11	34.96438723689674	22.946163357866066	23.479784452667648	18.60966495256955
12	23.427540232612255	24.859123118409585	30.197843614113694	21.51549303486446
13	29.524828552264754	21.457089015937907	25.12308190707373	23.89500052472361
14	22.777339240656495	20.439353358002947	27.49323562827667	29.290071773063886
15	24.69029900120002	27.627838640646456	23.882680926981287	23.799181431172233
16	23.067534209697804	27.622819545269962	24.905891961690614	24.403754283341623
17	23.21422867911098	26.504701979804985	25.941650734841193	24.33941860624284
18	24.080022631557334	25.74659043725446	27.074141164338872	23.09924576684933
19	25.426052755255217	25.58529496310965	26.675579363304934	22.313072918330192
20	25.29989094874591	25.424455770362698	26.520899969429145	22.754753311462245
21	24.046714089513284	25.78674320026647	26.804935139599294	23.361607570620954
22	23.608683947564142	25.753434658222417	27.034900964122592	23.602980430090845
23	23.580622641595525	25.965377367530103	26.8300306164818	23.623969374392576
24	23.910742232949904	25.83875927962293	26.69839343319812	23.552105054229045
25	23.945191478488614	25.74157134187796	27.15079643917997	23.16244074045345
26	24.060402531449196	25.79130601424511	26.564018561527263	23.584272892778436
27	23.773629672891865	26.184164297805744	26.997942170895634	23.044263858406758
28	23.59727691261755	25.977468824573492	26.696340166907735	23.728914095901224
29	23.819485953377168	26.01442761780045	26.588201475614042	23.577884953208343
30	23.97211208096257	26.009864803821813	26.628354238626045	23.38966887658957
31	23.835227661603465	25.585751244507517	26.750181371855653	23.828839722033372
32	23.215597523304574	25.93800048365828	26.884328102827578	23.96207389020957
33	23.248449783950758	25.767579381556192	27.297490908593147	23.6864799258999
34	23.534766361110222	25.686361292736454	26.689039664541912	24.089832681611405
35	23.438262845462056	25.96378038263758	26.759078859113995	23.838877912786373
36	24.066334189621426	25.63229194708961	26.731930115941104	23.569443747347865
37	24.405123127535212	25.65191204719775	26.620369314163433	23.322595511103607
38	23.845037711657532	26.029256763231018	26.420746202598067	23.704959322513382
39	23.374155309062207	25.571150239775875	27.122963273910283	23.931731177251635
40	23.26464777357492	25.6637753635422	27.32874618434681	23.742830678536066
41	23.627847766274417	25.34049999315578	27.24319342224737	23.788458818322436
42	23.534766361110222	25.88849395199007	27.129123072781447	23.447616614118257
43	23.867167359453923	25.476471849719157	26.878624585354277	23.77773620547264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	19.0
2	36.0
3	118.5
4	201.0
5	201.0
6	260.0
7	319.0
8	293.0
9	267.0
10	387.0
11	507.0
12	507.0
13	826.0
14	1145.0
15	1934.5
16	2724.0
17	3133.5
18	3543.0
19	3543.0
20	3145.5
21	2748.0
22	2855.5
23	2963.0
24	3803.0
25	4643.0
26	4643.0
27	6185.0
28	7727.0
29	9417.0
30	11107.0
31	12749.5
32	14392.0
33	14392.0
34	16701.5
35	19011.0
36	20248.5
37	21486.0
38	22980.0
39	24474.0
40	24474.0
41	26300.5
42	28127.0
43	29706.0
44	31285.0
45	32171.0
46	33057.0
47	33057.0
48	34325.0
49	35593.0
50	35861.0
51	36129.0
52	35393.5
53	34658.0
54	34658.0
55	32073.0
56	29488.0
57	27132.5
58	24777.0
59	23047.5
60	21318.0
61	21318.0
62	18749.0
63	16180.0
64	13461.0
65	10742.0
66	9351.5
67	7961.0
68	7961.0
69	6379.0
70	4797.0
71	3938.0
72	3079.0
73	2430.5
74	1782.0
75	1782.0
76	1386.0
77	990.0
78	722.0
79	454.0
80	374.0
81	294.0
82	294.0
83	214.5
84	135.0
85	123.5
86	112.0
87	82.5
88	53.0
89	53.0
90	34.0
91	15.0
92	9.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	438326.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.6208338101376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.624851672034	57.50015829208501
2	6.858091727489792	9.000673950085742
3	1.986400415756402	3.9104775468821726
4	0.9606601732343125	2.5215728630330725
5	0.5423723624691106	1.7795463330398613
6	0.3796042372902567	1.4945967941308784
7	0.26375321860950984	1.2115394287684478
8	0.18552928447750616	0.9739669074889737
9	0.14788656217394044	0.8733995567251853
>10	0.9300652769554218	11.53299645333744
>50	0.08052337969711976	3.723160320991533
>100	0.03886128321056281	4.2903874459124935
>500	7.002033010912217E-4	0.31198640704413205
>1k	7.002033010912217E-4	0.875537700475101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2242	0.511491447005197	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1569	0.35795275662406517	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	850	0.19391959409206846	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	508	0.11589547505737739	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	441	0.10061004822894376	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.844220967955357E-4	0.0	0.0
2	0.0	0.0	6.844220967955357E-4	0.0	0.0
3	0.0	0.0	0.0011407034946592262	4.562813978636905E-4	0.0
4	0.0	0.0	0.001825125591454762	6.844220967955357E-4	0.0
5	0.0	0.0	0.002737688387182143	6.844220967955357E-4	0.0
6	0.0	0.0	0.005475376774364286	6.844220967955357E-4	0.0
7	0.0	0.0	0.010266331451933036	6.844220967955357E-4	0.0
8	0.0	0.0	0.011407034946592263	6.844220967955357E-4	0.0
9	0.0	0.0	0.01391658263484256	0.0013688441935910715	0.0
10	0.0015969848925229168	0.0	0.015513567527365477	0.0015969848925229168	0.0
11	0.0015969848925229168	0.0	0.023954773387843752	0.0015969848925229168	0.0
12	0.0015969848925229168	0.0	0.026692461775025895	0.0015969848925229168	0.0
13	0.0015969848925229168	0.0	0.028061305968616966	0.0015969848925229168	0.0
14	0.0015969848925229168	0.0	0.029658290861139882	0.001825125591454762	0.0
15	0.0015969848925229168	0.0	0.03239597924832203	0.0020532662903866073	0.0
16	0.0015969848925229168	0.0	0.03558994903336786	0.0020532662903866073	0.0
17	0.0015969848925229168	0.0	0.035818089732299704	0.002509547688250298	0.0
18	0.0015969848925229168	0.0	0.036274371130163394	0.002509547688250298	0.0
19	0.0015969848925229168	0.0	0.036274371130163394	0.002737688387182143	0.0
20	0.0015969848925229168	0.0	0.03695879322695893	0.002965829086113988	0.0
21	0.0015969848925229168	0.0	0.03832763742055	0.0031939697850458335	0.0
22	0.0015969848925229168	0.0	0.03924020021627738	0.0034221104839776786	0.0
23	0.001825125591454762	0.0	0.03924020021627738	0.003878391881841369	0.0
24	0.0020532662903866073	0.0	0.03924020021627738	0.00524723607543244	0.0
25	0.0020532662903866073	0.0	0.03924020021627738	0.005703517473296131	0.0
26	0.0020532662903866073	0.0	0.03924020021627738	0.006387939570091667	0.0
27	0.0020532662903866073	0.0	0.03924020021627738	0.007528643064750893	0.0
28	0.0020532662903866073	0.0	0.03924020021627738	0.017110552419888393	0.0
29	0.0022814069893184524	0.0	0.03924020021627738	0.03718693392589077	0.0
30	0.0022814069893184524	0.0	0.03924020021627738	0.0670733654859625	0.0
31	0.0022814069893184524	0.0	0.03924020021627738	0.14464120312278989	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	320	0.0	27.171877	1
TCTACGC	50	9.0743415E-6	25.900002	10
GTATCAA	955	0.0	23.633507	1
GGCTACG	55	1.8991785E-5	23.545454	1
AATGACG	40	0.0019292914	23.125002	19
CAAGGCG	40	0.0019292914	23.125002	9
CGAGCGT	50	2.6984073E-4	22.2	6
ACGGGAT	85	5.162292E-8	21.764706	16
GCTACGA	70	5.090047E-6	21.142859	2
CGCGGAA	65	6.8896836E-5	19.923077	33
ATTCGCG	65	6.8896836E-5	19.923077	30
GCGAACG	90	2.14604E-6	18.5	22
CGGCCAA	70	1.2170144E-4	18.5	24
GATTTAG	60	9.2243904E-4	18.5	1
TACGAGC	60	9.2243904E-4	18.5	4
ATTGACG	80	1.6129543E-5	18.5	17
TCGCGGA	70	1.2170144E-4	18.5	32
CGAACCG	135	1.1459633E-9	17.814816	31
GCGAACC	135	1.1459633E-9	17.814816	30
CCGTATT	95	3.5960966E-6	17.526316	1
>>END_MODULE