FastQCFastQC Report
Fri 10 Feb 2017
ERR1632952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403829
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10750.2662017834281342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.18473165622082613No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5870.14535855523006025No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5500.13619626128881285No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5430.13446285432695523No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4900.12133848733003325No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4880.12084322819807393No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4720.11688115514239938No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4550.11267145252074517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4310.10672834293723332No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4240.10499493597537571No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4050.10028997422176218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG604.3082764E-827.75000224
ACTAGAC405.9307356E-527.7518
GCCGGCA751.3680619E-824.66666415
CCGGCAG802.7146598E-823.12516
AGCTTCG802.7146598E-823.12521
GGTATCA2100.022.9047641
CCGCTCT652.6756952E-622.7692328
GTATCAA5900.021.9491521
TACACGA603.7185855E-521.58333432
TGCTCGC1059.767973E-1021.14285910
GTCTAAG450.003821575520.55555535
GACCGCA555.1351584E-420.18181815
TGTGCAC555.1351584E-420.18181810
CCGTCCG555.1351584E-420.18181828
AGACCGC555.1351584E-420.18181814
TATACTG555.1351584E-420.1818185
CGTCCGC555.1351584E-420.18181829
CGAGTCG656.88797E-519.92307721
ATCGTAC656.88797E-519.92307725
AGTCGCC656.88797E-519.92307723
Produced by FastQC (version 0.11.5)