##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632952.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	403829
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.784698473858985	33.0	33.0	33.0	33.0	33.0
2	31.287745555668366	33.0	33.0	33.0	27.0	33.0
3	31.716761302432467	33.0	33.0	33.0	27.0	33.0
4	31.849671519380728	33.0	33.0	33.0	27.0	33.0
5	31.956231474213094	33.0	33.0	33.0	33.0	33.0
6	34.84929512244044	37.0	37.0	37.0	27.0	37.0
7	35.02227675575553	37.0	37.0	37.0	33.0	37.0
8	35.1962513836302	37.0	37.0	37.0	33.0	37.0
9	35.27873183946671	37.0	37.0	37.0	33.0	37.0
10	35.31354608014779	37.0	37.0	37.0	33.0	37.0
11	35.33850466410288	37.0	37.0	37.0	33.0	37.0
12	35.290021271379715	37.0	37.0	37.0	33.0	37.0
13	35.330246218077455	37.0	37.0	37.0	33.0	37.0
14	35.302615711105446	37.0	37.0	37.0	33.0	37.0
15	35.31024765432893	37.0	37.0	37.0	33.0	37.0
16	35.31217668864792	37.0	37.0	37.0	33.0	37.0
17	35.29062301122505	37.0	37.0	37.0	33.0	37.0
18	35.284281218040306	37.0	37.0	37.0	33.0	37.0
19	35.3016202402502	37.0	37.0	37.0	33.0	37.0
20	35.295298257430744	37.0	37.0	37.0	33.0	37.0
21	35.303177830220214	37.0	37.0	37.0	33.0	37.0
22	35.30353194049957	37.0	37.0	37.0	33.0	37.0
23	35.298230191491946	37.0	37.0	37.0	33.0	37.0
24	35.29156647987143	37.0	37.0	37.0	33.0	37.0
25	35.28551193698323	37.0	37.0	37.0	33.0	37.0
26	35.23918787407541	37.0	37.0	37.0	33.0	37.0
27	35.19989896713708	37.0	37.0	37.0	33.0	37.0
28	35.175772418523685	37.0	37.0	37.0	33.0	37.0
29	35.2227923205119	37.0	37.0	37.0	33.0	37.0
30	35.177037805605835	37.0	37.0	37.0	33.0	37.0
31	35.160914644564905	37.0	37.0	37.0	33.0	37.0
32	35.19867320078548	37.0	37.0	37.0	33.0	37.0
33	35.17740677365915	37.0	37.0	37.0	33.0	37.0
34	35.10995247988629	37.0	37.0	37.0	33.0	37.0
35	35.11267145252074	37.0	37.0	37.0	33.0	37.0
36	35.09845999172917	37.0	37.0	37.0	33.0	37.0
37	35.13636217309802	37.0	37.0	37.0	33.0	37.0
38	34.976757488937146	37.0	37.0	37.0	27.0	37.0
39	35.06318516005537	37.0	37.0	37.0	27.0	37.0
40	35.09155855572532	37.0	37.0	37.0	33.0	37.0
41	35.1316943557793	37.0	37.0	37.0	33.0	37.0
42	35.02715753450099	37.0	37.0	37.0	27.0	37.0
43	34.82630766983055	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	12.0
16	21.0
17	16.0
18	18.0
19	12.0
20	26.0
21	71.0
22	199.0
23	473.0
24	984.0
25	1832.0
26	3190.0
27	4663.0
28	6602.0
29	8975.0
30	12021.0
31	15412.0
32	19860.0
33	25620.0
34	37004.0
35	68952.0
36	197866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93960562515322	20.547806125860202	13.326680352327347	25.18590789665923
2	16.078835348625283	22.69574498116777	37.63796062194642	23.587459048260527
3	17.417025523179365	26.857655096587912	30.251913557471106	25.473405822761613
4	12.462205537492355	18.0338707720347	39.34090914718853	30.16301454328441
5	13.65800871160813	37.37869246636571	35.717098078642195	13.246200743383957
6	29.78612234386337	39.944877658612924	17.219912388659555	13.049087608864149
7	26.696448249135152	32.26142748539604	23.899967560526857	17.142156704941943
8	24.187217857063263	36.36390650498107	20.628780993935553	18.820094644020116
9	25.726730868758807	15.538507635657666	20.2872998224496	38.44746167313392
10	14.952120823417832	27.877888908424108	34.2781226707344	22.891867597423662
11	34.063427837030034	23.528027952425408	24.265964059044794	18.142580151499768
12	23.042674003105272	25.87431809008268	30.11769833271013	20.96530957410191
13	27.465833310633958	22.274527089436372	26.127395506513896	24.13224409341578
14	21.879310302132833	20.855114417240962	27.45444235059889	29.811132930027313
15	24.023782343516686	28.791642006889056	24.073803515844578	23.110772133749684
16	23.16203145390747	28.41524506659997	25.21463292631287	23.208090553179687
17	22.27526997813431	27.588905204925844	26.474076898885418	23.661747918054424
18	22.708621718598714	27.561913582234066	27.699100361786794	22.030364337380426
19	24.535880285962623	26.578081316596876	27.072102300726296	21.813936096714205
20	23.852175054292783	26.68233336387431	27.367029113808073	22.09846246802483
21	23.02459704478876	27.0696260050665	27.363562299884357	22.542214650260384
22	22.82253131894936	26.852454875702342	27.18675478977488	23.13825901557342
23	22.685096909830644	26.999794467460237	27.80706685255393	22.50804177015519
24	23.245234988076636	27.581971577078416	25.785171446330008	23.38762198851494
25	23.211062107971443	26.82422510518066	27.54334136478559	22.421371422062307
26	22.933469364508245	27.403925919139045	27.301407278823465	22.361197437529253
27	22.931488327980407	27.13351443308925	26.509983185952468	23.42501405297787
28	22.641018847086265	26.790795113773402	27.691919104373387	22.876266934766942
29	24.206532963209675	26.792280891169284	26.537965326908168	22.46322081871287
30	23.61420304138633	26.544651325189623	27.187992937604776	22.65315269581927
31	23.20462373925597	26.905199973256007	27.14441013399236	22.745766153495666
32	22.211876809243517	26.62760722979281	27.50471115249276	23.65580480847091
33	22.249516503272424	26.85815035571987	27.990065101812895	22.902268039194805
34	23.265292982920986	26.29454546355017	27.70306243484247	22.737099118686373
35	22.861161531242182	27.185269012379003	27.305864611011092	22.647704845367716
36	23.962865470285692	26.82422510518066	26.604577680156698	22.608331744376954
37	22.85868523558239	26.484972599788527	27.058482674597418	23.597859490031674
38	23.080561326700163	27.038424679753064	26.791042743339382	23.089971250207387
39	22.709612236862633	26.710315504830014	27.39030629301016	23.189765965297195
40	22.63755203316255	27.481929232422637	27.54309373521961	22.337424999195203
41	22.701688090751286	26.275725616535713	27.48465315764841	23.537933135064595
42	22.41097098029116	27.462861755842198	27.23132811165122	22.894839152215418
43	23.355925404069545	25.922110596316756	27.399468586951407	23.32249541266229
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	8.0
2	15.0
3	51.5
4	88.0
5	88.0
6	123.0
7	158.0
8	153.5
9	149.0
10	201.0
11	253.0
12	253.0
13	411.5
14	570.0
15	968.5
16	1367.0
17	1608.5
18	1850.0
19	1850.0
20	1818.5
21	1787.0
22	2078.5
23	2370.0
24	3358.0
25	4346.0
26	4346.0
27	5746.5
28	7147.0
29	9055.0
30	10963.0
31	13527.5
32	16092.0
33	16092.0
34	18891.0
35	21690.0
36	24317.5
37	26945.0
38	29217.0
39	31489.0
40	31489.0
41	33124.0
42	34759.0
43	35051.0
44	35343.0
45	34322.5
46	33302.0
47	33302.0
48	31545.5
49	29789.0
50	29812.0
51	29835.0
52	27935.5
53	26036.0
54	26036.0
55	24887.0
56	23738.0
57	20443.0
58	17148.0
59	15982.5
60	14817.0
61	14817.0
62	12960.0
63	11103.0
64	9269.5
65	7436.0
66	6196.0
67	4956.0
68	4956.0
69	4131.5
70	3307.0
71	2668.0
72	2029.0
73	1647.0
74	1265.0
75	1265.0
76	1002.0
77	739.0
78	565.5
79	392.0
80	313.5
81	235.0
82	235.0
83	190.0
84	145.0
85	122.0
86	99.0
87	76.0
88	53.0
89	53.0
90	36.5
91	20.0
92	11.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	403829.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.46985972214395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.36645289015927	55.2023991619169
2	6.416804144034213	8.017137094845785
3	1.8408558312262104	3.449940166361762
4	0.8240519905739083	2.0591364901962215
5	0.494950833549991	1.5459754570613078
6	0.34581243908184034	1.2961712735769007
7	0.23010008957614472	1.0062024222512163
8	0.18598623275578507	0.9294827096403125
9	0.1490256540978831	0.8378650535845953
>10	0.9556993131319789	11.746598140921577
>50	0.12271008112775854	5.320032938129001
>100	0.06555196516178928	7.714871758547674
>500	0.0015988284185802265	0.6057636303277553
>1k	3.9970710464505663E-4	0.2684237026390507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1075	0.2662017834281342	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	746	0.18473165622082613	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	587	0.14535855523006025	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	550	0.13619626128881285	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	543	0.13446285432695523	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	490	0.12133848733003325	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	488	0.12084322819807393	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	472	0.11688115514239938	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	455	0.11267145252074517	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	431	0.10672834293723332	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	424	0.10499493597537571	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	405	0.10028997422176218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	2.476295659796597E-4	0.0	0.0
5	0.0	0.0	2.476295659796597E-4	0.0	0.0
6	0.0	0.0	0.0014857773958779582	2.476295659796597E-4	0.0
7	0.0	0.0	0.004209702621654215	4.952591319593194E-4	0.0
8	0.0	0.0	0.005695480017532173	4.952591319593194E-4	0.0
9	0.0	0.0	0.006190739149491493	0.0012381478298982985	0.0
10	2.476295659796597E-4	0.0	0.006933627847430472	0.0012381478298982985	0.0
11	2.476295659796597E-4	0.0	0.00841940524330843	0.0012381478298982985	0.0
12	2.476295659796597E-4	0.0	0.010400441771145708	0.0012381478298982985	0.0
13	2.476295659796597E-4	0.0	0.011638589601044006	0.0012381478298982985	0.0
14	2.476295659796597E-4	0.0	0.011886219167023666	0.0012381478298982985	0.0
15	2.476295659796597E-4	0.0	0.013867255694860943	0.0014857773958779582	0.0
16	2.476295659796597E-4	0.0	0.015353033090738901	0.001733406961857618	0.0
17	2.476295659796597E-4	0.0	0.01584829222269822	0.0019810365278372776	0.0
18	2.476295659796597E-4	0.0	0.01634355135465754	0.0019810365278372776	0.0
19	2.476295659796597E-4	0.0	0.0165911809206372	0.0019810365278372776	0.0
20	2.476295659796597E-4	0.0	0.01708644005259652	0.0022286660938169375	0.0
21	2.476295659796597E-4	0.0	0.0178293287505355	0.0022286660938169375	0.0
22	2.476295659796597E-4	0.0	0.01807695831651516	0.0022286660938169375	0.0
23	2.476295659796597E-4	0.0	0.018324587882494817	0.0029715547917559164	0.0
24	2.476295659796597E-4	0.0	0.018324587882494817	0.003466813923715236	0.0
25	2.476295659796597E-4	0.0	0.018324587882494817	0.0037144434896948957	0.0
26	2.476295659796597E-4	0.0	0.018324587882494817	0.0037144434896948957	0.0
27	2.476295659796597E-4	0.0	0.018324587882494817	0.004704961753613534	0.0
28	2.476295659796597E-4	0.0	0.018324587882494817	0.00891466437526775	0.0
29	2.476295659796597E-4	0.0	0.018324587882494817	0.022534290504149034	0.0
30	2.476295659796597E-4	0.0	0.018324587882494817	0.04308754448046079	0.0
31	2.476295659796597E-4	0.0	0.018324587882494817	0.11291908208672483	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCCG	60	4.3082764E-8	27.750002	24
ACTAGAC	40	5.9307356E-5	27.75	18
GCCGGCA	75	1.3680619E-8	24.666664	15
CCGGCAG	80	2.7146598E-8	23.125	16
AGCTTCG	80	2.7146598E-8	23.125	21
GGTATCA	210	0.0	22.904764	1
CCGCTCT	65	2.6756952E-6	22.76923	28
GTATCAA	590	0.0	21.949152	1
TACACGA	60	3.7185855E-5	21.583334	32
TGCTCGC	105	9.767973E-10	21.142859	10
GTCTAAG	45	0.0038215755	20.555555	35
GACCGCA	55	5.1351584E-4	20.181818	15
TGTGCAC	55	5.1351584E-4	20.181818	10
CCGTCCG	55	5.1351584E-4	20.181818	28
AGACCGC	55	5.1351584E-4	20.181818	14
TATACTG	55	5.1351584E-4	20.181818	5
CGTCCGC	55	5.1351584E-4	20.181818	29
CGAGTCG	65	6.88797E-5	19.923077	21
ATCGTAC	65	6.88797E-5	19.923077	25
AGTCGCC	65	6.88797E-5	19.923077	23
>>END_MODULE