FastQCFastQC Report
Fri 10 Feb 2017
ERR1632951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386486
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17030.44063691828423285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12010.31074864290038967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6140.15886733283999938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2950.025.0847451
GTATCAA7600.022.3947371
TTCGCCG603.718054E-521.58333224
AGCGATT450.003821283320.55555530
CACGGTA500.007026640718.52
CGCTCTC801.61215E-518.529
AGCGCAG500.007026640718.510
ACTGTGC902.144703E-618.58
TATAGGA500.007026640718.52
ACGAGGG609.221701E-418.515
ACGGTAC609.221701E-418.53
TCGCCGC852.7155493E-517.41176425
AGCTTCG852.7155493E-517.41176421
TAAGCAG852.7155493E-517.4117645
CTCGTCT752.0627963E-417.26666613
CCGCTCT904.4336834E-516.44444528
CTTCTAA904.4336834E-516.4444451
TAAGACT904.4336834E-516.4444454
CTGCTCG1606.2391337E-1016.18759
GCTCGCC1059.310959E-615.85714411
Produced by FastQC (version 0.11.5)