##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632951.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386486
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.785045253903117	33.0	33.0	33.0	33.0	33.0
2	31.29802631919397	33.0	33.0	33.0	27.0	33.0
3	31.71740244148559	33.0	33.0	33.0	27.0	33.0
4	31.834573567994703	33.0	33.0	33.0	27.0	33.0
5	31.935130897367564	33.0	33.0	33.0	33.0	33.0
6	34.81568801974716	37.0	37.0	37.0	27.0	37.0
7	34.94545468658632	37.0	37.0	37.0	27.0	37.0
8	35.170707347743516	37.0	37.0	37.0	33.0	37.0
9	35.20247305206398	37.0	37.0	37.0	33.0	37.0
10	35.25746857583457	37.0	37.0	37.0	33.0	37.0
11	35.30855192684858	37.0	37.0	37.0	33.0	37.0
12	35.22264454598614	37.0	37.0	37.0	33.0	37.0
13	35.27633859958705	37.0	37.0	37.0	33.0	37.0
14	35.220605662300834	37.0	37.0	37.0	33.0	37.0
15	35.27176404837433	37.0	37.0	37.0	33.0	37.0
16	35.26724900772602	37.0	37.0	37.0	33.0	37.0
17	35.24917849546944	37.0	37.0	37.0	33.0	37.0
18	35.212007679450224	37.0	37.0	37.0	33.0	37.0
19	35.26028368427317	37.0	37.0	37.0	33.0	37.0
20	35.185318485016275	37.0	37.0	37.0	33.0	37.0
21	35.24913709681593	37.0	37.0	37.0	33.0	37.0
22	35.25857340240009	37.0	37.0	37.0	33.0	37.0
23	35.23703057808045	37.0	37.0	37.0	33.0	37.0
24	35.221671677628684	37.0	37.0	37.0	33.0	37.0
25	35.24165429019421	37.0	37.0	37.0	33.0	37.0
26	35.194948846788755	37.0	37.0	37.0	33.0	37.0
27	35.135448632033246	37.0	37.0	37.0	33.0	37.0
28	35.11997329786848	37.0	37.0	37.0	33.0	37.0
29	35.15264977256615	37.0	37.0	37.0	33.0	37.0
30	35.13094135363247	37.0	37.0	37.0	33.0	37.0
31	35.127629461351766	37.0	37.0	37.0	33.0	37.0
32	35.1447503919935	37.0	37.0	37.0	33.0	37.0
33	35.11390839512945	37.0	37.0	37.0	33.0	37.0
34	35.065588404237154	37.0	37.0	37.0	33.0	37.0
35	35.065200291860506	37.0	37.0	37.0	33.0	37.0
36	35.037693474019754	37.0	37.0	37.0	27.0	37.0
37	35.05277552097618	37.0	37.0	37.0	27.0	37.0
38	34.91609527900105	37.0	37.0	37.0	27.0	37.0
39	34.96768576352054	37.0	37.0	37.0	27.0	37.0
40	34.98565277914336	37.0	37.0	37.0	27.0	37.0
41	35.003560284201754	37.0	37.0	37.0	27.0	37.0
42	34.87128641140947	37.0	37.0	37.0	27.0	37.0
43	34.70561417489896	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	17.0
17	22.0
18	17.0
19	19.0
20	27.0
21	74.0
22	227.0
23	519.0
24	987.0
25	1889.0
26	3165.0
27	4602.0
28	6810.0
29	9087.0
30	11818.0
31	15248.0
32	19368.0
33	25109.0
34	35984.0
35	66068.0
36	185420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.345094000817625	20.064892389375036	13.72313615499656	24.866877454810783
2	15.773404470019612	23.127616524272547	37.983264594319074	23.11571441138877
3	18.01979890604058	26.815719068737288	30.117002944479232	25.047479080742903
4	12.721547481668157	17.931826767334393	38.99908405479112	30.34754169620633
5	14.265199774377338	36.931738795195685	34.823771106844745	13.979290323582225
6	29.66834503707767	39.47956717707757	17.481616410426252	13.370471375418514
7	26.386725521752407	32.27050915168984	23.48545613553919	17.85730919101856
8	24.44073006525463	35.881765445578885	20.683284776162655	18.994219713003837
9	26.03069710157677	15.336389933917399	20.11741693101432	38.51549603349151
10	14.916452342387565	28.21292362465911	33.84572791769948	23.024896115253853
11	34.45558183220091	23.51003658606004	23.994142090528506	18.04023949121055
12	23.134085063883298	25.289919945353777	30.4577656111735	21.11822937958943
13	28.64916193600803	22.004678047846493	26.10857831849019	23.237581697655287
14	22.282566509524276	20.928054315033403	27.71303488354041	29.076344291901908
15	23.88779929932779	28.43207774667129	24.324555093845575	23.35556786015535
16	22.90199386264962	28.115119305744578	25.338822104811044	23.64406472679476
17	22.50094440678317	27.188824433485305	26.56810337243781	23.742127787293718
18	23.525819822710268	26.598376137816114	27.5808179338967	22.294986105576918
19	24.870499836992803	26.373788442530905	27.138628566105886	21.617083154370402
20	24.4992056633358	25.905983657881528	27.28792246032198	22.306888218460692
21	23.309511858126815	26.698767872574948	27.275502864269342	22.716217405028903
22	23.03886816081307	26.43252278219651	27.557789932882432	22.97081912410799
23	23.04456047567053	26.592166339789795	27.63256625078269	22.73070693375698
24	23.071210858866813	26.65452306163742	26.938103838172662	23.336162241323102
25	23.33021118488121	26.534466966461917	27.724419513255334	22.41090233540154
26	23.382994468104926	26.756467245902826	27.246006323644323	22.614531962347925
27	23.200581651081798	26.772767965721915	27.132677509664	22.893972873532288
28	22.97858137164089	26.649089488364393	27.391419094093965	22.980910045900757
29	23.66088292978271	26.556718742722897	26.861775070765827	22.920623256728575
30	23.464239325615935	26.509886515941066	27.36968480100185	22.65618935744115
31	23.58972899406447	26.285557562240285	27.11767049776706	23.007042945928184
32	22.710525090171444	26.321263900891623	27.58185290023442	23.386358108702517
33	22.821525229891897	26.307550596916833	27.86207003617207	23.008854137019195
34	23.05620384696988	26.097711171944134	27.40978974658849	23.436295234497496
35	22.926056830001603	26.9634605134468	27.327768664324193	22.782713992227404
36	23.42982669488675	26.537571865475073	27.188306950316445	22.844294489321733
37	23.18479841443157	26.200431580962828	27.36528619406654	23.24948381053906
38	23.28156776700838	26.69359304088634	26.83538342915397	23.18945576295131
39	22.816867881372158	26.40768359009123	27.452223366435007	23.3232251621016
40	23.03990312715079	26.72929937953768	27.714069849878133	22.5167276434334
41	23.115973152973197	26.10288600363273	27.43928628721351	23.34185455618056
42	22.702762842638542	26.81364913606185	27.377188306950316	23.10639971434929
43	23.107175939102582	26.02785094414804	27.474216401111555	23.390756715637824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	15.0
2	24.0
3	91.5
4	159.0
5	159.0
6	226.0
7	293.0
8	285.5
9	278.0
10	337.0
11	396.0
12	396.0
13	639.5
14	883.0
15	1552.5
16	2222.0
17	2595.5
18	2969.0
19	2969.0
20	2747.0
21	2525.0
22	2812.0
23	3099.0
24	4125.0
25	5151.0
26	5151.0
27	6590.0
28	8029.0
29	9733.5
30	11438.0
31	13277.5
32	15117.0
33	15117.0
34	17267.5
35	19418.0
36	21007.0
37	22596.0
38	24061.5
39	25527.0
40	25527.0
41	27171.5
42	28816.0
43	29129.5
44	29443.0
45	29630.0
46	29817.0
47	29817.0
48	29743.5
49	29670.0
50	29143.5
51	28617.0
52	27481.5
53	26346.0
54	26346.0
55	24991.0
56	23636.0
57	21066.5
58	18497.0
59	17261.0
60	16025.0
61	16025.0
62	13982.0
63	11939.0
64	10038.5
65	8138.0
66	6943.5
67	5749.0
68	5749.0
69	4769.5
70	3790.0
71	3132.0
72	2474.0
73	2024.0
74	1574.0
75	1574.0
76	1174.5
77	775.0
78	610.0
79	445.0
80	362.0
81	279.0
82	279.0
83	213.5
84	148.0
85	127.5
86	107.0
87	77.5
88	48.0
89	48.0
90	33.0
91	18.0
92	11.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	386486.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.90669800353334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.56248874989218	64.40142830983349
2	6.1841598060915715	8.893650231644317
3	1.6129390818832074	3.479433703772164
4	0.7417118064663347	2.1333618749291956
5	0.41373554374467825	1.4875178398688116
6	0.2571714870942594	1.109541147456389
7	0.20176567311371452	1.0155812326847247
8	0.14648712434764677	0.8426724329497817
9	0.0978866511589195	0.6334835277115477
>10	0.7049867277536921	9.600309178013633
>50	0.046651358260596056	2.2995536885640924
>100	0.028931074885027247	3.1886381686310084
>500	3.616384360628406E-4	0.15966594646382637
>1k	7.232768721256812E-4	0.7551627174771202
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1703	0.44063691828423285	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.31074864290038967	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	614	0.15886733283999938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.174831688599329E-4	0.0	0.0
2	0.0	0.0	5.174831688599329E-4	2.5874158442996644E-4	0.0
3	0.0	0.0	7.762247532898993E-4	2.5874158442996644E-4	0.0
4	0.0	0.0	0.001293707922149832	5.174831688599329E-4	0.0
5	0.0	0.0	0.001293707922149832	5.174831688599329E-4	0.0
6	0.0	0.0	0.0020699326754397315	5.174831688599329E-4	0.0
7	0.0	0.0	0.00646853961074916	5.174831688599329E-4	0.0
8	0.0	0.0	0.008020989117328958	5.174831688599329E-4	0.0
9	0.0	0.0	0.009573438623908758	0.0015524495065797985	0.0
10	0.0	0.0	0.011384629714918522	0.0015524495065797985	0.0
11	0.0	0.0	0.020699326754397313	0.0015524495065797985	0.0
12	0.0	0.0	0.021475551507687214	0.0015524495065797985	0.0
13	0.0	0.0	0.02302800101426701	0.0015524495065797985	0.0
14	0.0	0.0	0.024839192105276776	0.0015524495065797985	0.0
15	0.0	0.0	0.027685349534006407	0.0015524495065797985	0.0
16	0.0	0.0	0.03130773171602594	0.0015524495065797985	0.0
17	0.0	0.0	0.03130773171602594	0.0015524495065797985	0.0
18	0.0	0.0	0.03182521488488587	0.0015524495065797985	0.0
19	0.0	0.0	0.032860181222605735	0.0015524495065797985	0.0
20	0.0	0.0	0.033636405975895636	0.0015524495065797985	0.0
21	0.0	0.0	0.0338951475603256	0.0015524495065797985	0.0
22	0.0	0.0	0.03441263072918553	0.0018111910910097648	0.0
23	0.0	0.0	0.03493011389804546	0.0018111910910097648	0.0
24	0.0	0.0	0.03518885548247543	0.0023286742598696976	0.0
25	0.0	0.0	0.03518885548247543	0.002587415844299664	0.0
26	0.0	0.0	0.03518885548247543	0.003104899013159597	0.0
27	0.0	0.0	0.035706338651335365	0.003104899013159597	0.0
28	0.0	0.0	0.035706338651335365	0.011125888130488556	0.0
29	0.0	0.0	0.035706338651335365	0.02820283270286634	0.0
30	0.0	0.0	0.035706338651335365	0.05071335054827342	0.0
31	0.0	0.0	0.035706338651335365	0.11514000507133505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	295	0.0	25.084745	1
GTATCAA	760	0.0	22.394737	1
TTCGCCG	60	3.718054E-5	21.583332	24
AGCGATT	45	0.0038212833	20.555555	30
CACGGTA	50	0.0070266407	18.5	2
CGCTCTC	80	1.61215E-5	18.5	29
AGCGCAG	50	0.0070266407	18.5	10
ACTGTGC	90	2.144703E-6	18.5	8
TATAGGA	50	0.0070266407	18.5	2
ACGAGGG	60	9.221701E-4	18.5	15
ACGGTAC	60	9.221701E-4	18.5	3
TCGCCGC	85	2.7155493E-5	17.411764	25
AGCTTCG	85	2.7155493E-5	17.411764	21
TAAGCAG	85	2.7155493E-5	17.411764	5
CTCGTCT	75	2.0627963E-4	17.266666	13
CCGCTCT	90	4.4336834E-5	16.444445	28
CTTCTAA	90	4.4336834E-5	16.444445	1
TAAGACT	90	4.4336834E-5	16.444445	4
CTGCTCG	160	6.2391337E-10	16.1875	9
GCTCGCC	105	9.310959E-6	15.857144	11
>>END_MODULE