FastQCFastQC Report
Fri 10 Feb 2017
ERR1632950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410660
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8080.1967564408513125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.14854137242487703No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG5530.13466127696878197No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG5290.12881702625042615No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT4940.12029416061949058No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT4890.11907660838649979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTCA250.005492280729.625
TCTAAGC451.3211898E-424.6666663
TAAAGCC1450.024.2413794
GGTATCA2100.023.7857131
AATATAG551.8988005E-523.5454545
TTAACGG551.8988005E-523.54545435
CAGTCCG400.001929085723.1259
CCACCGG400.001929085723.12520
AGCCTTA1450.022.9655177
AACGGCC652.675877E-622.7692337
CGGCGTC450.003821684320.55555524
GGGCGTG450.003821684320.55555536
GCTTACC450.003821684320.5555558
GTACGGA450.003821684320.5555556
GTATCAA4800.020.4270821
ATATAGC759.2420105E-619.7333346
GGCCGTA1551.8189894E-1219.09677314
CCGTATG1551.8189894E-1219.09677316
AAAGCCT1650.019.0606065
ATCCTAA500.007027371318.51
Produced by FastQC (version 0.11.5)