##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632950.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410660
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.584829299176935	33.0	33.0	33.0	27.0	33.0
2	31.150862026980956	33.0	33.0	33.0	27.0	33.0
3	31.57785759509083	33.0	33.0	33.0	27.0	33.0
4	31.71378025617299	33.0	33.0	33.0	27.0	33.0
5	31.82092485267618	33.0	33.0	33.0	33.0	33.0
6	34.67551745969902	37.0	37.0	37.0	27.0	37.0
7	34.82054741148395	37.0	37.0	37.0	27.0	37.0
8	35.04008181951006	37.0	37.0	37.0	33.0	37.0
9	35.10336287926752	37.0	37.0	37.0	33.0	37.0
10	35.15076462280232	37.0	37.0	37.0	33.0	37.0
11	35.21078994788876	37.0	37.0	37.0	33.0	37.0
12	35.11002776019091	37.0	37.0	37.0	33.0	37.0
13	35.15597087615059	37.0	37.0	37.0	33.0	37.0
14	35.104322310427115	37.0	37.0	37.0	33.0	37.0
15	35.16576486631276	37.0	37.0	37.0	33.0	37.0
16	35.11714313544051	37.0	37.0	37.0	33.0	37.0
17	35.13992597282424	37.0	37.0	37.0	33.0	37.0
18	35.10950421273073	37.0	37.0	37.0	33.0	37.0
19	35.14616227536161	37.0	37.0	37.0	33.0	37.0
20	35.068290069643986	37.0	37.0	37.0	33.0	37.0
21	35.13659231481031	37.0	37.0	37.0	33.0	37.0
22	35.16688501436712	37.0	37.0	37.0	33.0	37.0
23	35.133446159840254	37.0	37.0	37.0	33.0	37.0
24	35.138781473725224	37.0	37.0	37.0	33.0	37.0
25	35.1184605269566	37.0	37.0	37.0	33.0	37.0
26	35.08161009107291	37.0	37.0	37.0	33.0	37.0
27	34.997637948668	37.0	37.0	37.0	27.0	37.0
28	35.01864559489602	37.0	37.0	37.0	27.0	37.0
29	35.04160132469683	37.0	37.0	37.0	27.0	37.0
30	35.02922125359178	37.0	37.0	37.0	27.0	37.0
31	35.0171942726343	37.0	37.0	37.0	27.0	37.0
32	35.04474260945795	37.0	37.0	37.0	27.0	37.0
33	35.00880777285346	37.0	37.0	37.0	27.0	37.0
34	34.96380460721765	37.0	37.0	37.0	27.0	37.0
35	34.951804412409295	37.0	37.0	37.0	27.0	37.0
36	34.93348755661618	37.0	37.0	37.0	27.0	37.0
37	34.95908293965811	37.0	37.0	37.0	27.0	37.0
38	34.81132810597575	37.0	37.0	37.0	27.0	37.0
39	34.896322992256366	37.0	37.0	37.0	27.0	37.0
40	34.93242585106901	37.0	37.0	37.0	27.0	37.0
41	34.955274436273314	37.0	37.0	37.0	27.0	37.0
42	34.833107680319486	37.0	37.0	37.0	27.0	37.0
43	34.64449179369795	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	17.0
17	12.0
18	9.0
19	19.0
20	34.0
21	84.0
22	229.0
23	625.0
24	1272.0
25	2178.0
26	3594.0
27	5522.0
28	7724.0
29	10132.0
30	13567.0
31	17350.0
32	21472.0
33	28202.0
34	39934.0
35	70955.0
36	187722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55286611795646	20.08449812496956	12.659377587298495	25.703258169775484
2	16.352213509959576	22.659621097745095	36.73915160960405	24.24901378269128
3	18.664832221302294	25.272731700189937	29.849997564895535	26.212438513612234
4	13.200701310086203	17.027224467929674	38.28641698728876	31.48565723469537
5	14.389519310378414	36.568207276092146	35.17459699021088	13.867676423318558
6	31.381434763551358	38.96435007061803	16.504651049530025	13.149564116300589
7	27.642088345590025	31.43865971850192	22.79476939560707	18.124482540300978
8	25.223786100423705	35.6221691910583	20.390590756343446	18.763453952174547
9	25.78264257536648	14.855111284274095	19.896508060195785	39.46573808016364
10	14.447231286222179	28.389421906199775	34.258997711001804	22.904349096576244
11	35.03823114011591	22.91555057711976	23.780256172989823	18.26596210977451
12	23.295913894706082	25.024594555106418	30.323381873082354	21.35610967710515
13	28.399892855403497	21.91399211026153	25.96210977450933	23.72400525982565
14	22.400038961671456	20.123946817318465	26.821214630107633	30.65479959090245
15	23.758583743242585	29.08634880436371	23.744216626893294	23.410850825500415
16	23.715238883748114	27.870744654945696	24.594068085520867	23.819948375785323
17	22.603370184580918	27.159450640432475	26.17055471679735	24.066624458189256
18	23.718648030000487	26.810743680903908	27.037695417133396	22.43291287196221
19	24.73019042516924	26.05001704573126	26.521453270345297	22.6983392587542
20	24.005503336093117	26.86090683290313	26.653679442848098	22.479910388155652
21	23.24891637851264	26.74475234987581	27.189645935810645	22.81668533580091
22	23.031218039253883	26.622266595236937	26.90181658793162	23.444698777577557
23	23.39818828227731	26.459845127355962	26.962450689134563	23.17951590123216
24	23.287634539521747	26.969025471192715	25.62947450445624	24.1138654848293
25	23.757366191009595	27.041835094725563	26.41016899624994	22.790629718014905
26	23.7965713729119	26.259436029805677	26.761311060244488	23.18268153703794
27	23.442020162664978	26.55676228510203	26.564311108946576	23.436906443286418
28	23.151268689426775	26.25529635221351	27.11099206155944	23.482442896800272
29	23.898602250036525	26.269419958116202	26.401402620172405	23.430575171674864
30	23.67968635854478	26.561876004480595	26.71139141869186	23.047046218282766
31	23.512638192178446	26.57161642234452	26.88209224175717	23.033653143719864
32	23.316855793113525	26.52242731213169	26.81439633760288	23.346320557151902
33	23.454439195441484	26.27234208347538	27.150440753908345	23.122777967174795
34	23.731067062776994	26.036136950275168	26.77762626016656	23.45516972678128
35	23.36093118394779	26.876978522378607	26.70968684556567	23.052403448107924
36	24.02888033896654	26.079238299323045	26.920810402766275	22.97107095894414
37	23.818243802659133	26.179077582428285	26.439633760288316	23.563044854624263
38	23.794866799785712	26.323479277260997	26.690693030731016	23.190960892222275
39	23.52116105780938	26.26454974918424	26.626162762382506	23.588126430623873
40	22.874153801198073	27.234695368431304	26.87064725076706	23.020503579603567
41	23.900793844055908	25.73369697560025	26.731115764866313	23.634393415477525
42	23.296887936492475	26.565041640286367	26.640773389178396	23.49729703404276
43	23.594944723128624	25.737349632299228	27.075926557249307	23.591779087322845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	9.5
2	16.0
3	56.5
4	97.0
5	97.0
6	121.0
7	145.0
8	148.5
9	152.0
10	187.0
11	222.0
12	222.0
13	339.0
14	456.0
15	833.5
16	1211.0
17	1396.0
18	1581.0
19	1581.0
20	1666.0
21	1751.0
22	2180.0
23	2609.0
24	3431.0
25	4253.0
26	4253.0
27	5662.5
28	7072.0
29	8796.0
30	10520.0
31	12444.0
32	14368.0
33	14368.0
34	17062.5
35	19757.0
36	22328.0
37	24899.0
38	26424.5
39	27950.0
40	27950.0
41	29259.5
42	30569.0
43	32288.0
44	34007.0
45	33710.0
46	33413.0
47	33413.0
48	32462.5
49	31512.0
50	31828.0
51	32144.0
52	30761.5
53	29379.0
54	29379.0
55	27437.0
56	25495.0
57	22468.0
58	19441.0
59	18606.0
60	17771.0
61	17771.0
62	16712.5
63	15654.0
64	12597.5
65	9541.0
66	7794.0
67	6047.0
68	6047.0
69	4828.0
70	3609.0
71	2918.0
72	2227.0
73	1762.0
74	1297.0
75	1297.0
76	975.5
77	654.0
78	494.0
79	334.0
80	271.5
81	209.0
82	209.0
83	164.5
84	120.0
85	109.5
86	99.0
87	75.5
88	52.0
89	52.0
90	37.5
91	23.0
92	12.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	410660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.51238916612648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.24032194010556	53.66360634157936
2	6.50867455324012	8.007282441491412
3	2.024939599503388	3.736766180476582
4	1.0353380260665044	2.547444623115681
5	0.599094461425143	1.8425865829227173
6	0.4383055346251922	1.617673237171917
7	0.3191004120591694	1.3740040110758462
8	0.23947340146266025	1.1784464852565761
9	0.18309138530950075	1.01361496915109
>10	1.2434218694884156	14.746070574501099
>50	0.11920238435571182	4.916402188800923
>100	0.04744175163153699	4.743023480195921
>500	0.0015946807271104868	0.6130788842608864
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	808	0.1967564408513125	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.14854137242487703	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	553	0.13466127696878197	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	529	0.12881702625042615	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	494	0.12029416061949058	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	489	0.11907660838649979	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.4351044659815907E-4	0.0	0.0
2	0.0	0.0	2.4351044659815907E-4	0.0	0.0
3	0.0	0.0	2.4351044659815907E-4	0.0	0.0
4	0.0	0.0	4.8702089319631813E-4	0.0	0.0
5	0.0	0.0	4.8702089319631813E-4	0.0	0.0
6	0.0	0.0	0.0017045731261871135	0.0	0.0
7	0.0	0.0	0.0029221253591779086	0.0	0.0
8	0.0	0.0	0.003165635805776068	0.0	0.0
9	0.0	0.0	0.003896167145570545	0.0	0.0
10	0.0	0.0	0.003896167145570545	0.0	0.0
11	0.0	0.0	0.005844250718355817	0.0	0.0
12	0.0	0.0	0.006574782058150295	0.0	0.0
13	0.0	0.0	0.0070618029513466124	0.0	0.0
14	0.0	0.0	0.007548823844542931	0.0	0.0
15	0.0	0.0	0.00779233429114109	0.0	0.0
16	0.0	0.0	0.008035844737739248	0.0	0.0
17	0.0	0.0	0.008522865630935567	0.0	0.0
18	0.0	0.0	0.008766376077533727	0.0	0.0
19	0.0	0.0	0.009009886524131885	2.4351044659815907E-4	0.0
20	0.0	0.0	0.009253396970730044	2.4351044659815907E-4	0.0
21	0.0	0.0	0.009253396970730044	4.8702089319631813E-4	0.0
22	0.0	0.0	0.009253396970730044	7.305313397944771E-4	0.0
23	0.0	0.0	0.009253396970730044	9.740417863926363E-4	0.0
24	0.0	0.0	0.009253396970730044	9.740417863926363E-4	0.0
25	0.0	0.0	0.009253396970730044	9.740417863926363E-4	0.0
26	0.0	0.0	0.009253396970730044	9.740417863926363E-4	0.0
27	0.0	0.0	0.009253396970730044	0.0024351044659815906	0.0
28	0.0	0.0	0.009253396970730044	0.00779233429114109	0.0
29	0.0	0.0	0.009253396970730044	0.022402961087030635	0.0
30	0.0	0.0	0.009253396970730044	0.03847465056250913	0.0
31	0.0	0.0	0.009253396970730044	0.09253396970730045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTCA	25	0.0054922807	29.6	25
TCTAAGC	45	1.3211898E-4	24.666666	3
TAAAGCC	145	0.0	24.241379	4
GGTATCA	210	0.0	23.785713	1
AATATAG	55	1.8988005E-5	23.545454	5
TTAACGG	55	1.8988005E-5	23.545454	35
CAGTCCG	40	0.0019290857	23.125	9
CCACCGG	40	0.0019290857	23.125	20
AGCCTTA	145	0.0	22.965517	7
AACGGCC	65	2.675877E-6	22.76923	37
CGGCGTC	45	0.0038216843	20.555555	24
GGGCGTG	45	0.0038216843	20.555555	36
GCTTACC	45	0.0038216843	20.555555	8
GTACGGA	45	0.0038216843	20.555555	6
GTATCAA	480	0.0	20.427082	1
ATATAGC	75	9.2420105E-6	19.733334	6
GGCCGTA	155	1.8189894E-12	19.096773	14
CCGTATG	155	1.8189894E-12	19.096773	16
AAAGCCT	165	0.0	19.060606	5
ATCCTAA	50	0.0070273713	18.5	1
>>END_MODULE