FastQCFastQC Report
Fri 10 Feb 2017
ERR1632949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294938
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28030.9503692301432843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21770.7381212322589832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11250.3814360984342472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.19326095654001857No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4050.13731699543632897No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4010.13596077819745167No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3370.11426130237541449No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3340.1132441394462565No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.10544589032271189No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG3110.10544589032271189No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3000.10171629291579926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT250.005489801529.64
TTCGCCG1100.026.9090924
GGTATCA3900.025.6153831
GCTTCGC1250.025.1622
GCCGGCA1400.023.78571315
GTATCAA10600.023.735851
AGCTTCG1350.023.29629521
CTCACGG400.001927802823.1259
CGCTCTC1400.022.46428529
CCGGCAG1450.021.68965316
CCGCTCT1450.021.68965328
CTTCGCC1500.020.96666723
TGCTCGC1700.020.67647210
TCACGGT450.003819167420.55555510
TTTAACC450.003819167420.5555553
CGGAGTT909.418363E-820.55555524
GCCGCTC1450.020.41379227
CTGCTCG1750.020.0857149
GCTCTCT1750.020.08571430
TTGTCCG1600.019.6562513
Produced by FastQC (version 0.11.5)