##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632949.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294938
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15303555323492	33.0	33.0	33.0	27.0	33.0
2	30.789443883121198	33.0	33.0	33.0	27.0	33.0
3	31.2310960269616	33.0	33.0	33.0	27.0	33.0
4	31.400816442777803	33.0	33.0	33.0	27.0	33.0
5	31.50211569889265	33.0	33.0	33.0	27.0	33.0
6	34.23208945608908	37.0	33.0	37.0	27.0	37.0
7	34.416362082878436	37.0	37.0	37.0	27.0	37.0
8	34.61992011880463	37.0	37.0	37.0	27.0	37.0
9	34.72895659426727	37.0	37.0	37.0	27.0	37.0
10	34.78331378120147	37.0	37.0	37.0	27.0	37.0
11	34.767364666472275	37.0	37.0	37.0	27.0	37.0
12	34.73271670656206	37.0	37.0	37.0	27.0	37.0
13	34.74439034644569	37.0	37.0	37.0	27.0	37.0
14	34.75221232937092	37.0	37.0	37.0	27.0	37.0
15	34.748675992920546	37.0	37.0	37.0	27.0	37.0
16	34.761746536560224	37.0	37.0	37.0	27.0	37.0
17	34.7535651560667	37.0	37.0	37.0	27.0	37.0
18	34.747713078680945	37.0	37.0	37.0	27.0	37.0
19	34.74959822064298	37.0	37.0	37.0	27.0	37.0
20	34.75148675314812	37.0	37.0	37.0	27.0	37.0
21	34.74313245495664	37.0	37.0	37.0	27.0	37.0
22	34.74658402782958	37.0	37.0	37.0	27.0	37.0
23	34.72382331201812	37.0	37.0	37.0	27.0	37.0
24	34.71945629250894	37.0	37.0	37.0	27.0	37.0
25	34.70418867694227	37.0	37.0	37.0	27.0	37.0
26	34.66710969763137	37.0	37.0	37.0	27.0	37.0
27	34.58476696797293	37.0	37.0	37.0	27.0	37.0
28	34.549600254968844	37.0	37.0	37.0	27.0	37.0
29	34.57498525113753	37.0	37.0	37.0	27.0	37.0
30	34.52876197709349	37.0	37.0	37.0	27.0	37.0
31	34.517752883656904	37.0	37.0	37.0	27.0	37.0
32	34.53670601957022	37.0	37.0	37.0	27.0	37.0
33	34.46573178091667	37.0	37.0	37.0	27.0	37.0
34	34.387681478819275	37.0	37.0	37.0	27.0	37.0
35	34.38041215441889	37.0	37.0	37.0	27.0	37.0
36	34.35150777451532	37.0	37.0	37.0	27.0	37.0
37	34.3555255680855	37.0	37.0	37.0	27.0	37.0
38	34.20608399053361	37.0	37.0	37.0	27.0	37.0
39	34.2423322867857	37.0	37.0	37.0	27.0	37.0
40	34.24484806976381	37.0	37.0	37.0	27.0	37.0
41	34.21775763041724	37.0	37.0	37.0	27.0	37.0
42	34.09356203676705	37.0	37.0	37.0	27.0	37.0
43	33.81493737666899	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	12.0
17	16.0
18	15.0
19	21.0
20	43.0
21	146.0
22	322.0
23	734.0
24	1511.0
25	2593.0
26	3868.0
27	5549.0
28	7326.0
29	9537.0
30	12075.0
31	14771.0
32	18215.0
33	23264.0
34	31430.0
35	52908.0
36	110577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77827204361594	19.389498809919374	14.287748611572601	24.54448053489208
2	16.22374872006998	23.82500728966766	36.61108436349335	23.340159626769015
3	18.775471455017666	26.47912442615058	30.306030419952666	24.439373698879084
4	13.598790254222923	17.939702581559512	37.32614990269141	31.135357261526153
5	15.66736059782056	35.697332998799745	32.936413754755236	15.698892648624458
6	29.784903945914053	38.94072652557487	16.778441570770806	14.495927957740271
7	26.623561562091012	32.509883433128316	21.998860777519344	18.86769422726132
8	25.814577979100694	34.130223979277005	19.950633692504866	20.10456434911744
9	27.15791115420868	14.471516047440481	19.291851168720207	39.078721629630635
10	15.794505963965308	27.72616617729828	32.100305826987366	24.379022031749045
11	34.990404763034945	23.08892038326699	23.178091666723173	18.74258318697489
12	23.623609029694375	25.110362177813645	29.75777960113651	21.50824919135547
13	29.88051726125491	21.67675918328598	25.412120513463847	23.030603041995267
14	23.077731591046255	20.61823162834223	28.052675477557997	28.251361303053525
15	24.960839227227417	27.600716082702125	24.299344268965005	23.139100421105454
16	22.671205473692776	27.595630268056336	25.028650089171283	24.7045141690796
17	23.04992913764927	26.183130013765606	26.011229478737903	24.755711369847223
18	24.729265133689115	24.703836060460166	27.46814584760187	23.098752958248852
19	25.878320189327926	25.00627250472981	27.50272938719324	21.612677918749025
20	25.29718110246899	24.601441658924927	27.403047420135756	22.698329818470324
21	23.978259837660794	25.428056066020655	27.649878957611428	22.94380513870712
22	23.557493439299108	25.31752436105215	27.8685689873804	23.25641321226834
23	23.37236978619235	25.3026059714245	27.916714699360547	23.4083095430226
24	23.368979243095158	25.1113793407428	28.18524571265825	23.334395703503787
25	23.592755087509918	25.3236273386271	28.544982335270465	22.538635238592516
26	23.699557195071506	25.453485139249604	27.58851012755223	23.258447538126656
27	23.646664722755293	25.50536044863666	28.21169194881636	22.636282879791686
28	23.413056303358672	25.398558341075073	27.683106279963926	23.50527907560233
29	23.387627230129723	25.60334714414555	27.24403094887739	23.764994676847337
30	23.54257504967146	25.71048830601686	27.714638330767823	23.032298313543865
31	23.629712007269323	25.2534430965152	27.70141521268877	23.415429683526707
32	22.809200577748545	25.524347489980947	27.830933959001552	23.835517973268956
33	23.037045073879934	25.48637340729238	28.246953597027176	23.229627921800514
34	23.09502336084194	25.399914558313952	27.626145155931077	23.87891692491303
35	22.982457330015123	25.863062745390557	27.94892485878388	23.20555506581044
36	23.386610067200564	25.46704731163838	27.9580793251463	23.188263296014753
37	23.76736805701537	25.651492856125696	27.662084912761326	22.919054174097607
38	23.259464701055816	25.687432612955945	27.45661800107141	23.59648468491683
39	23.245563474357322	25.376180756633598	27.638012056771256	23.740243712237827
40	23.10078728410717	25.970203907261862	27.965877574269847	22.96313123436112
41	23.477137567895625	25.49756219951312	27.448819751947866	23.57648048064339
42	23.022465738562005	26.02445259681696	27.686835877370836	23.2662457872502
43	23.14622056160956	25.694552753460048	27.488828160494748	23.670398524435644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	21.5
2	39.0
3	164.5
4	290.0
5	290.0
6	346.0
7	402.0
8	419.5
9	437.0
10	580.5
11	724.0
12	724.0
13	1133.0
14	1542.0
15	2699.5
16	3857.0
17	4090.5
18	4324.0
19	4324.0
20	3587.0
21	2850.0
22	2517.0
23	2184.0
24	2542.0
25	2900.0
26	2900.0
27	3600.0
28	4300.0
29	5226.5
30	6153.0
31	7172.5
32	8192.0
33	8192.0
34	9659.0
35	11126.0
36	11983.5
37	12841.0
38	14138.5
39	15436.0
40	15436.0
41	16576.5
42	17717.0
43	18952.0
44	20187.0
45	21524.5
46	22862.0
47	22862.0
48	24106.5
49	25351.0
50	25564.0
51	25777.0
52	25119.0
53	24461.0
54	24461.0
55	22410.5
56	20360.0
57	18648.0
58	16936.0
59	15463.5
60	13991.0
61	13991.0
62	12172.5
63	10354.0
64	8383.5
65	6413.0
66	5686.0
67	4959.0
68	4959.0
69	4044.5
70	3130.0
71	2566.5
72	2003.0
73	1665.0
74	1327.0
75	1327.0
76	985.0
77	643.0
78	482.0
79	321.0
80	274.0
81	227.0
82	227.0
83	174.5
84	122.0
85	117.0
86	112.0
87	82.0
88	52.0
89	52.0
90	40.0
91	28.0
92	15.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	294938.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.69391500555999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.51388209245442	57.699494990751944
2	7.5874123305710475	10.120684661744866
3	2.3154462899549073	4.6327853418657545
4	1.0849610509562195	2.894412004672686
5	0.620354921058347	2.0686949189173136
6	0.40154669301152934	1.6068432608684777
7	0.282587439110301	1.3192803851962889
8	0.18919488852865693	1.0094518252013316
9	0.13773478368205933	0.826746476058041
>10	0.7762307987666596	9.380145005970732
>50	0.05633535768158536	2.5648187916081153
>100	0.032264795763089764	3.5966895559506504
>500	5.121396152871392E-4	0.1946925970457617
>1k	0.0015364188458614173	2.0852601841480265
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2803	0.9503692301432843	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2177	0.7381212322589832	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1125	0.3814360984342472	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	570	0.19326095654001857	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	405	0.13731699543632897	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	401	0.13596077819745167	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	337	0.11426130237541449	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	334	0.1132441394462565	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.10544589032271189	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	311	0.10544589032271189	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	300	0.10171629291579926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0016952715485966541	0.0	0.0
2	6.781086194386617E-4	0.0	0.0016952715485966541	0.0	0.0
3	6.781086194386617E-4	0.0	0.00406865171663197	3.3905430971933084E-4	0.0
4	6.781086194386617E-4	0.0	0.0047467603360706314	6.781086194386617E-4	0.0
5	6.781086194386617E-4	0.0	0.0054248689555092934	6.781086194386617E-4	0.0
6	6.781086194386617E-4	0.0	0.011866900840176579	6.781086194386617E-4	0.0
7	6.781086194386617E-4	0.0	0.021021367202598513	6.781086194386617E-4	0.0
8	6.781086194386617E-4	0.0	0.025090018919230482	6.781086194386617E-4	0.0
9	6.781086194386617E-4	0.0	0.02780245339698513	0.0010171629291579926	0.0
10	0.0023733801680353157	0.0	0.03390543097193308	0.0010171629291579926	0.0
11	0.0027124344777546467	0.0	0.046111386121828996	0.0010171629291579926	0.0
12	0.0027124344777546467	0.0	0.0539096352453736	0.0010171629291579926	0.0
13	0.0027124344777546467	0.0	0.058995449891163565	0.0010171629291579926	0.0
14	0.0027124344777546467	0.0	0.06408126453695354	0.0010171629291579926	0.0
15	0.0027124344777546467	0.0	0.0691670791827435	0.0010171629291579926	0.0
16	0.0027124344777546467	0.0	0.07527005675769145	0.0010171629291579926	0.0
17	0.0027124344777546467	0.0	0.0769653283062881	0.0010171629291579926	0.0
18	0.0027124344777546467	0.0	0.07899965416460408	0.0013562172388773234	0.0
19	0.0027124344777546467	0.0	0.08035587140348141	0.0016952715485966541	0.0
20	0.0027124344777546467	0.0	0.08374641450067472	0.002034325858315985	0.0
21	0.0027124344777546467	0.0	0.08645884897842937	0.0030514887874739777	0.0
22	0.0027124344777546467	0.0	0.08781506621730668	0.0030514887874739777	0.0
23	0.0027124344777546467	0.0	0.08984939207562267	0.0033905430971933083	0.0
24	0.0027124344777546467	0.0	0.08984939207562267	0.0033905430971933083	0.0
25	0.0027124344777546467	0.0	0.08984939207562267	0.0037295974069126393	0.0
26	0.0027124344777546467	0.0	0.08984939207562267	0.004407706026351301	0.0
27	0.0027124344777546467	0.0	0.090188446385342	0.007120140504105948	0.0
28	0.0027124344777546467	0.0	0.090188446385342	0.017969878415124534	0.0
29	0.0027124344777546467	0.0	0.090188446385342	0.04984098352874163	0.0
30	0.0027124344777546467	0.0	0.090188446385342	0.09222277224365799	0.0
31	0.0027124344777546467	0.0	0.090188446385342	0.1858017617261933	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAGT	25	0.0054898015	29.6	4
TTCGCCG	110	0.0	26.90909	24
GGTATCA	390	0.0	25.615383	1
GCTTCGC	125	0.0	25.16	22
GCCGGCA	140	0.0	23.785713	15
GTATCAA	1060	0.0	23.73585	1
AGCTTCG	135	0.0	23.296295	21
CTCACGG	40	0.0019278028	23.125	9
CGCTCTC	140	0.0	22.464285	29
CCGGCAG	145	0.0	21.689653	16
CCGCTCT	145	0.0	21.689653	28
CTTCGCC	150	0.0	20.966667	23
TGCTCGC	170	0.0	20.676472	10
TCACGGT	45	0.0038191674	20.555555	10
TTTAACC	45	0.0038191674	20.555555	3
CGGAGTT	90	9.418363E-8	20.555555	24
GCCGCTC	145	0.0	20.413792	27
CTGCTCG	175	0.0	20.085714	9
GCTCTCT	175	0.0	20.085714	30
TTGTCCG	160	0.0	19.65625	13
>>END_MODULE