FastQCFastQC Report
Fri 10 Feb 2017
ERR1632948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448926
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10520.23433706223297382No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8900.19825093667998733No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG8380.18666773588520158No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC8300.18488570499369605No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC8070.17976236618061775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.16595162677145006No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC6570.14634928696488952No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT6140.13677087092304746No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5750.12808347032695813No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC5580.12429665468250892No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5450.12140085448381248No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT5300.11805954656223966No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA5150.11471823864066684No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5060.11271345388772314No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4660.10380329943019562No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4570.10179851467725194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCA551.850458E-830.27272815
CGGTGGA250.005492819529.69
GTATCAA5800.024.5603451
AGCTTCG802.7164788E-823.12521
CGCTCTC652.6768012E-622.7692329
GCTTCGC753.7302743E-722.222
GTCGCCC1059.786163E-1021.14285737
CCGCTCT705.090502E-621.14285728
TTCGCCG806.9406815E-720.812524
CCGGCAG909.446012E-820.55555716
AGACGCT450.003822230320.55555723
ATCGGCG450.003822230320.55555710
CGGCCTT1254.0927262E-1019.2424
TCGCCGC902.1462747E-618.525
CTTCGCC902.1462747E-618.523
CGGCGTA500.00702836718.512
ATTACCC1306.9303496E-1018.53
TGTATCG500.00702836718.55
GCCGCTC801.6130969E-518.527
GTCAACC1103.8360668E-818.515
Produced by FastQC (version 0.11.5)