##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632948.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448926
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.703164886863313	33.0	33.0	33.0	27.0	33.0
2	31.215857401888062	33.0	33.0	33.0	27.0	33.0
3	31.657916894989373	33.0	33.0	33.0	27.0	33.0
4	31.784142598111938	33.0	33.0	33.0	27.0	33.0
5	31.89935757786361	33.0	33.0	33.0	33.0	33.0
6	34.76537781282438	37.0	37.0	37.0	27.0	37.0
7	34.92141021014599	37.0	37.0	37.0	27.0	37.0
8	35.09573515456891	37.0	37.0	37.0	33.0	37.0
9	35.19596993713886	37.0	37.0	37.0	33.0	37.0
10	35.226427072613305	37.0	37.0	37.0	33.0	37.0
11	35.25399286296628	37.0	37.0	37.0	33.0	37.0
12	35.19070180831585	37.0	37.0	37.0	33.0	37.0
13	35.242184680771445	37.0	37.0	37.0	33.0	37.0
14	35.204207374934846	37.0	37.0	37.0	33.0	37.0
15	35.226941634033224	37.0	37.0	37.0	33.0	37.0
16	35.22220143186182	37.0	37.0	37.0	33.0	37.0
17	35.20185732169667	37.0	37.0	37.0	33.0	37.0
18	35.207978597808996	37.0	37.0	37.0	33.0	37.0
19	35.2002512663557	37.0	37.0	37.0	33.0	37.0
20	35.21059372814228	37.0	37.0	37.0	33.0	37.0
21	35.2068336429612	37.0	37.0	37.0	33.0	37.0
22	35.221769289370634	37.0	37.0	37.0	33.0	37.0
23	35.21582398880884	37.0	37.0	37.0	33.0	37.0
24	35.20769347286635	37.0	37.0	37.0	33.0	37.0
25	35.20042724190624	37.0	37.0	37.0	33.0	37.0
26	35.16199774573092	37.0	37.0	37.0	33.0	37.0
27	35.10543608523454	37.0	37.0	37.0	33.0	37.0
28	35.078427179535154	37.0	37.0	37.0	33.0	37.0
29	35.11942280019424	37.0	37.0	37.0	33.0	37.0
30	35.08697424519854	37.0	37.0	37.0	33.0	37.0
31	35.072270262805006	37.0	37.0	37.0	33.0	37.0
32	35.099996435938216	37.0	37.0	37.0	33.0	37.0
33	35.08005551026227	37.0	37.0	37.0	33.0	37.0
34	35.01972930950758	37.0	37.0	37.0	27.0	37.0
35	35.02381016024913	37.0	37.0	37.0	27.0	37.0
36	34.9884457572072	37.0	37.0	37.0	27.0	37.0
37	35.022148416442796	37.0	37.0	37.0	27.0	37.0
38	34.88646012928634	37.0	37.0	37.0	27.0	37.0
39	34.96120964256915	37.0	37.0	37.0	27.0	37.0
40	35.00035417863969	37.0	37.0	37.0	27.0	37.0
41	35.01949764549168	37.0	37.0	37.0	27.0	37.0
42	34.93826376730241	37.0	37.0	37.0	27.0	37.0
43	34.72713320235406	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	23.0
17	19.0
18	15.0
19	25.0
20	31.0
21	94.0
22	272.0
23	615.0
24	1293.0
25	2326.0
26	3898.0
27	5707.0
28	8089.0
29	10848.0
30	14074.0
31	18046.0
32	22611.0
33	29283.0
34	41303.0
35	75189.0
36	215152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68020119128765	20.669553556710905	13.501557049491453	25.148688202509987
2	15.662269505441879	23.45442233241113	38.111849168905344	22.771458993241648
3	17.651906995807774	26.83783073379577	31.29558100889679	24.214681261499667
4	12.909922793511624	18.06288787016123	38.79258496946045	30.2346043668667
5	13.475272093841747	37.75588849832712	35.672917140018626	13.095922267812513
6	29.526469841354704	39.42431492049915	17.699353568294107	13.349861669852048
7	25.589740848157604	32.764642725081636	24.120901885834193	17.524714540926567
8	23.82998534279592	36.76842063057163	21.07073326116108	18.330860765471368
9	25.361195386322024	15.93091066233633	20.10353599479647	38.60435795654518
10	14.338220553053288	28.93260804676049	34.57295857223685	22.156212827949375
11	33.23487612657765	23.80659618734491	24.73948935904804	18.2190383270294
12	21.95974392216089	26.216792968106102	30.362910591055098	21.46055251867791
13	27.104689859798718	22.219252170736382	27.317419797472187	23.358638171992713
14	21.659248963080774	21.29571466121365	27.503864779495952	29.54117159620962
15	23.454867840134007	29.096109381056124	24.76065097588467	22.688371802925204
16	23.002677501414485	29.0078988519266	24.929052895131935	23.06037075152698
17	22.412379768603287	28.078792495867916	26.308567559018638	23.20026017651016
18	22.002735417418464	28.06965958754895	27.764486797378634	22.163118197653954
19	24.33608211598348	27.09399767444969	27.64575898923208	20.924161220334756
20	23.974329845007862	26.523302281445048	27.848910510863707	21.653457362683383
21	22.219252170736382	28.04382013962212	26.804417654580043	22.93251003506146
22	22.219252170736382	27.801909446100247	26.866343228059858	23.112495155103513
23	22.15665833567225	27.954941348908285	27.22631346814397	22.662086847275496
24	23.48672164231967	27.97209339623902	26.18516191978188	22.35602304165943
25	23.381359065859407	27.06303488770978	27.24235174616752	22.313254300263292
26	22.97394225328896	27.236114638047248	26.974156096995944	22.815787011667847
27	22.628228260336893	27.10112579801571	26.995317713832566	23.27532822781483
28	21.88489862471766	27.409194388384723	27.838886587098987	22.86702039979863
29	23.375567465462012	27.644645219924886	26.72712206466099	22.25266524995211
30	22.421512676922255	27.990581966738393	27.036749932060072	22.55115542427928
31	23.152590850162387	27.33457184480293	27.153472955453683	22.359364349581
32	22.186507353104968	27.41454048105924	27.314078489550614	23.084873676285177
33	22.184279814490584	26.99910452947702	27.830199186502895	22.986416469529498
34	22.385426551369267	27.19423691209687	27.469115177111597	22.951221359422266
35	22.88194490851499	27.751121565692344	26.688808400493624	22.678125125299047
36	22.896201155647034	27.743993442126317	26.654504305832145	22.705301096394507
37	23.44751696270655	26.54958723709476	26.876367151824574	23.12652864837412
38	23.3229975541626	27.266409163202844	26.93138735559981	22.479205927034744
39	22.661195831829744	27.035413408891447	27.539059889603184	22.764330869675625
40	22.804203810873062	27.02093440789796	27.433920067004365	22.740941714224615
41	22.309690238480286	27.25460320854662	27.690532515381154	22.74517403759194
42	22.924713649911123	26.885277306282106	27.30360905806302	22.88639998574375
43	22.71198371223765	26.416825935677597	27.462878068991326	23.408312283093426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	13.0
2	20.0
3	72.5
4	125.0
5	125.0
6	160.0
7	195.0
8	183.5
9	172.0
10	257.5
11	343.0
12	343.0
13	462.0
14	581.0
15	1192.5
16	1804.0
17	2066.5
18	2329.0
19	2329.0
20	2390.5
21	2452.0
22	3072.0
23	3692.0
24	4834.0
25	5976.0
26	5976.0
27	7645.0
28	9314.0
29	12559.5
30	15805.0
31	17355.5
32	18906.0
33	18906.0
34	21228.5
35	23551.0
36	25867.0
37	28183.0
38	31259.5
39	34336.0
40	34336.0
41	36540.0
42	38744.0
43	36475.5
44	34207.0
45	34781.0
46	35355.0
47	35355.0
48	34872.0
49	34389.0
50	35116.5
51	35844.0
52	35345.5
53	34847.0
54	34847.0
55	29637.5
56	24428.0
57	21166.5
58	17905.0
59	15972.5
60	14040.0
61	14040.0
62	12315.5
63	10591.0
64	8758.0
65	6925.0
66	5996.0
67	5067.0
68	5067.0
69	4212.0
70	3357.0
71	2808.5
72	2260.0
73	1840.0
74	1420.0
75	1420.0
76	1070.5
77	721.0
78	594.0
79	467.0
80	349.0
81	231.0
82	231.0
83	190.0
84	149.0
85	127.5
86	106.0
87	82.0
88	58.0
89	58.0
90	38.5
91	19.0
92	12.5
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	448926.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.00453048028498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.55073386841038	51.363437417218115
2	6.292842275912772	7.300267232016165
3	1.6729999459422293	2.9112472907376343
4	0.7894468146741506	1.8316596729732262
5	0.49327464214101013	1.4306082007609944
6	0.3213452549940631	1.1183688382798846
7	0.2400372348040788	0.9746272971817558
8	0.17202689345223374	0.7982671347743062
9	0.13954878191578957	0.728501541670895
>10	1.0663624305909762	12.622525426705785
>50	0.1488639275285411	5.9418515687917965
>100	0.10710469590571892	10.811644461407758
>500	0.005026574176080671	1.9310512967829894
>1k	3.866595520062055E-4	0.23594262069868815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.23433706223297382	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	890	0.19825093667998733	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	838	0.18666773588520158	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	830	0.18488570499369605	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	807	0.17976236618061775	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	745	0.16595162677145006	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	657	0.14634928696488952	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	614	0.13677087092304746	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	575	0.12808347032695813	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	558	0.12429665468250892	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	545	0.12140085448381248	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	530	0.11805954656223966	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	515	0.11471823864066684	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	506	0.11271345388772314	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	466	0.10380329943019562	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	457	0.10179851467725194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.2275386143818802E-4	0.0	0.0
2	0.0	0.0	2.2275386143818802E-4	0.0	0.0
3	0.0	0.0	4.4550772287637604E-4	0.0	0.0
4	0.0	0.0	6.682615843145641E-4	2.2275386143818802E-4	0.0
5	0.0	2.2275386143818802E-4	6.682615843145641E-4	4.4550772287637604E-4	0.0
6	0.0	2.2275386143818802E-4	6.682615843145641E-4	4.4550772287637604E-4	0.0
7	0.0	2.2275386143818802E-4	0.0026730463372582566	4.4550772287637604E-4	0.0
8	0.0	2.2275386143818802E-4	0.0037868156444491965	4.4550772287637604E-4	0.0
9	0.0	2.2275386143818802E-4	0.004009569505887384	8.910154457527521E-4	0.0
10	2.2275386143818802E-4	2.2275386143818802E-4	0.0044550772287637605	0.0011137693071909401	0.0
11	2.2275386143818802E-4	2.2275386143818802E-4	0.009355662180403897	0.0011137693071909401	0.0
12	2.2275386143818802E-4	2.2275386143818802E-4	0.009801169903280274	0.0011137693071909401	0.0
13	2.2275386143818802E-4	2.2275386143818802E-4	0.010692185349033026	0.0011137693071909401	0.0
14	2.2275386143818802E-4	2.2275386143818802E-4	0.011805954656223966	0.0011137693071909401	0.0
15	2.2275386143818802E-4	2.2275386143818802E-4	0.012251462379100343	0.0011137693071909401	0.0
16	2.2275386143818802E-4	2.2275386143818802E-4	0.014033493270605846	0.0011137693071909401	0.0
17	2.2275386143818802E-4	2.2275386143818802E-4	0.014479000993482222	0.0011137693071909401	0.0
18	2.2275386143818802E-4	2.2275386143818802E-4	0.01470175485492041	0.0011137693071909401	0.0
19	2.2275386143818802E-4	2.2275386143818802E-4	0.01470175485492041	0.0011137693071909401	0.0
20	2.2275386143818802E-4	2.2275386143818802E-4	0.014924508716358599	0.0011137693071909401	0.0
21	2.2275386143818802E-4	2.2275386143818802E-4	0.01581552416211135	0.0011137693071909401	0.0
22	2.2275386143818802E-4	2.2275386143818802E-4	0.01581552416211135	0.0017820308915055042	0.0
23	2.2275386143818802E-4	2.2275386143818802E-4	0.01581552416211135	0.0022275386143818803	0.0
24	2.2275386143818802E-4	2.2275386143818802E-4	0.016038278023549537	0.0024502924758200684	0.0
25	2.2275386143818802E-4	2.2275386143818802E-4	0.016038278023549537	0.0024502924758200684	0.0
26	2.2275386143818802E-4	2.2275386143818802E-4	0.016261031884987728	0.0026730463372582566	0.0
27	2.2275386143818802E-4	2.2275386143818802E-4	0.016261031884987728	0.0037868156444491965	0.0
28	2.2275386143818802E-4	2.2275386143818802E-4	0.016261031884987728	0.010469431487594837	0.0
29	2.2275386143818802E-4	2.2275386143818802E-4	0.016483785746425915	0.027398724956897127	0.0
30	2.2275386143818802E-4	2.2275386143818802E-4	0.016483785746425915	0.050342372685030494	0.0
31	2.2275386143818802E-4	2.2275386143818802E-4	0.016483785746425915	0.11249070002628496	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGCA	55	1.850458E-8	30.272728	15
CGGTGGA	25	0.0054928195	29.6	9
GTATCAA	580	0.0	24.560345	1
AGCTTCG	80	2.7164788E-8	23.125	21
CGCTCTC	65	2.6768012E-6	22.76923	29
GCTTCGC	75	3.7302743E-7	22.2	22
GTCGCCC	105	9.786163E-10	21.142857	37
CCGCTCT	70	5.090502E-6	21.142857	28
TTCGCCG	80	6.9406815E-7	20.8125	24
CCGGCAG	90	9.446012E-8	20.555557	16
AGACGCT	45	0.0038222303	20.555557	23
ATCGGCG	45	0.0038222303	20.555557	10
CGGCCTT	125	4.0927262E-10	19.24	24
TCGCCGC	90	2.1462747E-6	18.5	25
CTTCGCC	90	2.1462747E-6	18.5	23
CGGCGTA	50	0.007028367	18.5	12
ATTACCC	130	6.9303496E-10	18.5	3
TGTATCG	50	0.007028367	18.5	5
GCCGCTC	80	1.6130969E-5	18.5	27
GTCAACC	110	3.8360668E-8	18.5	15
>>END_MODULE