FastQCFastQC Report
Fri 10 Feb 2017
ERR1632947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431706
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10760.2492436982576105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.1721078697076251No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5930.13736200099141543No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5810.13458233149411866No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5680.13157102287204717No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5580.12925463162429987No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4780.11072350164232139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA303.5957884E-430.83333421
AGTAACG250.00549258829.623
TTGCCGA454.000638E-628.77777910
CATTCCG358.86039E-426.4285736
GGTATCA2550.025.3921571
CGAGTTC451.321343E-424.66666814
GCCGAGT451.321343E-424.66666812
CGTCGGG400.001929245223.12528
CGAAGGT400.001929245223.1254
CGGACTC400.001929245223.12514
CCGAGTT502.6983157E-422.213
TCGCCAT1107.4578566E-1121.86363613
TAGGACC855.161928E-821.7647064
AACGGCC806.9394264E-720.812537
GCCGGCA450.003821996720.55555715
GTCGGGG450.003821996720.55555729
CGAAACA555.135946E-420.18181817
ACCTACT555.135946E-420.1818187
TAGTCCG555.135946E-420.18181816
TGCCGAG555.135946E-420.18181811
Produced by FastQC (version 0.11.5)