##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632947.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431706
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78937980940733	33.0	33.0	33.0	33.0	33.0
2	31.312571518579773	33.0	33.0	33.0	27.0	33.0
3	31.716239755759705	33.0	33.0	33.0	27.0	33.0
4	31.84175804830139	33.0	33.0	33.0	27.0	33.0
5	31.951068551282585	33.0	33.0	33.0	33.0	33.0
6	34.85837815550398	37.0	37.0	37.0	27.0	37.0
7	34.99331721125025	37.0	37.0	37.0	33.0	37.0
8	35.19361556244296	37.0	37.0	37.0	33.0	37.0
9	35.23188697863824	37.0	37.0	37.0	33.0	37.0
10	35.31324095565037	37.0	37.0	37.0	33.0	37.0
11	35.342763362102914	37.0	37.0	37.0	33.0	37.0
12	35.26657957035575	37.0	37.0	37.0	33.0	37.0
13	35.307123366365076	37.0	37.0	37.0	33.0	37.0
14	35.26439289701788	37.0	37.0	37.0	33.0	37.0
15	35.31363474216249	37.0	37.0	37.0	33.0	37.0
16	35.2911541651031	37.0	37.0	37.0	33.0	37.0
17	35.29038049042636	37.0	37.0	37.0	33.0	37.0
18	35.25273449986796	37.0	37.0	37.0	33.0	37.0
19	35.29538389552149	37.0	37.0	37.0	33.0	37.0
20	35.22315186724298	37.0	37.0	37.0	33.0	37.0
21	35.28840692508327	37.0	37.0	37.0	33.0	37.0
22	35.3116704423844	37.0	37.0	37.0	33.0	37.0
23	35.28505510694779	37.0	37.0	37.0	33.0	37.0
24	35.28446442717961	37.0	37.0	37.0	33.0	37.0
25	35.294049654162784	37.0	37.0	37.0	33.0	37.0
26	35.23700851968701	37.0	37.0	37.0	33.0	37.0
27	35.18527655395107	37.0	37.0	37.0	33.0	37.0
28	35.18737288803028	37.0	37.0	37.0	33.0	37.0
29	35.21213742685995	37.0	37.0	37.0	33.0	37.0
30	35.17327301450524	37.0	37.0	37.0	33.0	37.0
31	35.18090784005782	37.0	37.0	37.0	33.0	37.0
32	35.211305842402005	37.0	37.0	37.0	33.0	37.0
33	35.17884393545607	37.0	37.0	37.0	33.0	37.0
34	35.12398020875318	37.0	37.0	37.0	33.0	37.0
35	35.132550856369846	37.0	37.0	37.0	33.0	37.0
36	35.10761027180535	37.0	37.0	37.0	33.0	37.0
37	35.136632337748374	37.0	37.0	37.0	33.0	37.0
38	34.97482082713699	37.0	37.0	37.0	27.0	37.0
39	35.05099998610165	37.0	37.0	37.0	27.0	37.0
40	35.100848725753174	37.0	37.0	37.0	33.0	37.0
41	35.1412373235489	37.0	37.0	37.0	33.0	37.0
42	35.00389153729621	37.0	37.0	37.0	27.0	37.0
43	34.824169689557245	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	12.0
16	20.0
17	27.0
18	17.0
19	15.0
20	29.0
21	68.0
22	221.0
23	516.0
24	1106.0
25	1964.0
26	3196.0
27	4990.0
28	7080.0
29	9715.0
30	12869.0
31	16368.0
32	21273.0
33	27976.0
34	39870.0
35	73990.0
36	210382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80114707694588	20.62746406118979	13.456148397288894	25.11524046457543
2	15.828364674106915	23.24197486252218	38.17644415412341	22.7532163092475
3	17.606194956752972	26.688765039170175	31.20086355065716	24.50417645341969
4	12.85759289887099	17.865167498251125	38.98486469958722	30.292374903290664
5	13.552741912319958	37.577193738331175	35.73566269637207	13.134401652976793
6	29.35632120007598	39.598013462865936	17.671285550814673	13.374379786243415
7	25.79533293491404	32.65694708899112	23.963299097070692	17.58442087902415
8	24.030474443255365	36.47366494790436	21.11182147109375	18.384039137746523
9	25.533812363043367	15.787596188146564	20.15167729890249	38.526914149907576
10	14.41304962173331	28.736686541303573	34.554303160020936	22.29596067694218
11	33.18577921085183	23.72725882892524	24.648024349904794	18.43893761031813
12	22.037219774568804	26.1054977229874	30.67759076779104	21.17969173465275
13	27.3128008413133	22.38676321385387	26.939861850425984	23.360574094406843
14	21.593630850625196	21.438432637026125	27.833062315557346	29.134874196791333
15	23.591981580056796	28.843472177824726	24.736047217319193	22.828499024799285
16	22.90910944022089	29.03179478626658	25.033703492654723	23.025392280857808
17	22.404599426461527	28.129792034393773	26.279458705693227	23.18614983345147
18	22.028880766076913	28.11288237828522	27.808740207456	22.049496648181865
19	24.280181419762524	26.85531356988321	27.71631619667088	21.148188813683387
20	24.028158052007615	26.367713212232395	28.042232445228926	21.561896290531056
21	22.358503240631357	27.710293579426736	26.960477732530936	22.97072544741097
22	21.98741736274224	27.701028014435753	27.102935794267395	23.208618828554616
23	22.207011253028682	27.552547335455145	27.710756857676287	22.529684553839882
24	23.136115782500127	27.71562127929656	26.52800748657651	22.620255451626804
25	23.173178042464084	27.178450148943956	27.316507067309697	22.331864741282264
26	22.91049927496954	27.386925361241214	27.179376705443055	22.52319865834619
27	22.805103473197036	27.212037822036294	26.64753327496027	23.335325429806396
28	22.00316882322692	27.59030451279343	27.69129917119521	22.71522749278444
29	23.321195443195137	27.69801670581368	26.754550550606197	22.226237300384984
30	22.581571717789423	27.622039072887567	27.306314945819608	22.490074263503406
31	23.14746609961409	27.214122574159266	27.141156249855225	22.49725507637142
32	22.070575808536365	27.24469893862953	27.36213997489032	23.322585277943787
33	22.30870082880479	26.977155749514715	27.918768791724	22.7953746299565
34	22.500498024118265	27.070043038549386	27.493710997762367	22.935747939569985
35	22.790741847461003	27.68990933644656	27.158992462462876	22.360356353629555
36	23.0290985068542	27.437191051317335	26.919709246570584	22.614001195257885
37	23.300116282840637	26.55951040754588	27.05128026944263	23.08909304017086
38	23.27926876161091	26.967195267149403	27.060777473558396	22.692758497681293
39	22.58388810903717	27.175902118571436	27.43186335144751	22.808346420943884
40	22.675848841572737	27.192580135555218	27.524519001357405	22.607052021514644
41	22.429848091061974	26.801573292935473	27.821943637568157	22.946634978434396
42	22.74742533112813	26.792539367069256	27.40661468684707	23.05342061495555
43	22.717080605782638	26.402690720073384	27.658638054601976	23.221590619542003
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	12.5
2	18.0
3	74.0
4	130.0
5	130.0
6	166.0
7	202.0
8	195.5
9	189.0
10	250.0
11	311.0
12	311.0
13	467.0
14	623.0
15	1181.5
16	1740.0
17	2043.5
18	2347.0
19	2347.0
20	2496.0
21	2645.0
22	3272.5
23	3900.0
24	5023.0
25	6146.0
26	6146.0
27	7875.0
28	9604.0
29	12490.5
30	15377.0
31	17166.5
32	18956.0
33	18956.0
34	21264.0
35	23572.0
36	25327.0
37	27082.0
38	29461.5
39	31841.0
40	31841.0
41	33606.0
42	35371.0
43	34112.0
44	32853.0
45	32842.5
46	32832.0
47	32832.0
48	32171.0
49	31510.0
50	31994.0
51	32478.0
52	32439.0
53	32400.0
54	32400.0
55	28559.0
56	24718.0
57	21843.0
58	18968.0
59	16439.0
60	13910.0
61	13910.0
62	12394.0
63	10878.0
64	9013.5
65	7149.0
66	6079.5
67	5010.0
68	5010.0
69	4145.0
70	3280.0
71	2803.0
72	2326.0
73	1907.5
74	1489.0
75	1489.0
76	1136.5
77	784.0
78	617.0
79	450.0
80	342.0
81	234.0
82	234.0
83	187.5
84	141.0
85	128.5
86	116.0
87	95.5
88	75.0
89	75.0
90	54.5
91	34.0
92	21.5
93	9.0
94	5.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	431706.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.44932739559194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.51607204868642	54.11186346438443
2	5.677000148806433	6.8634168124004855
3	1.4727954910116579	2.670884904687459
4	0.7409436257308387	1.7915817525392168
5	0.4493772905888803	1.3582277481475644
6	0.28945846519962865	1.0498541718166747
7	0.22040858310923112	0.9326485420817914
8	0.17617080954337386	0.8519525554907105
9	0.12724087408300366	0.6922462729990301
>10	1.083419664268743	13.541920599307874
>50	0.1603728667295891	6.674476557663415
>100	0.08442706204905899	8.501038034277109
>500	0.001927558494270753	0.709138373812474
>1k	3.8551169885415066E-4	0.2507502103917917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.2492436982576105	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	743	0.1721078697076251	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	593	0.13736200099141543	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	581	0.13458233149411866	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	568	0.13157102287204717	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	558	0.12925463162429987	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	478	0.11072350164232139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.3163912477473095E-4	0.0	0.0	0.0	0.0
3	2.3163912477473095E-4	0.0	0.0	0.0	0.0
4	2.3163912477473095E-4	0.0	2.3163912477473095E-4	2.3163912477473095E-4	0.0
5	2.3163912477473095E-4	0.0	2.3163912477473095E-4	2.3163912477473095E-4	0.0
6	2.3163912477473095E-4	0.0	9.265564990989238E-4	2.3163912477473095E-4	0.0
7	2.3163912477473095E-4	0.0	0.0016214738734231166	2.3163912477473095E-4	0.0
8	4.632782495494619E-4	0.0	0.0020847521229725783	2.3163912477473095E-4	0.0
9	4.632782495494619E-4	0.0	0.0025480303725220403	9.265564990989238E-4	0.0
10	6.949173743241928E-4	0.0	0.0030113086220715022	9.265564990989238E-4	0.0
11	6.949173743241928E-4	0.0	0.0057909781193682735	9.265564990989238E-4	0.0
12	6.949173743241928E-4	0.0	0.0062542563689177355	9.265564990989238E-4	0.0
13	6.949173743241928E-4	0.0	0.0064858954936924664	9.265564990989238E-4	0.0
14	6.949173743241928E-4	0.0	0.006717534618467197	9.265564990989238E-4	0.0
15	6.949173743241928E-4	0.0	0.00741245199279139	9.265564990989238E-4	0.0
16	6.949173743241928E-4	0.0	0.009265564990989237	9.265564990989238E-4	0.0
17	6.949173743241928E-4	0.0	0.009497204115763968	9.265564990989238E-4	0.0
18	6.949173743241928E-4	0.0	0.00996048236531343	9.265564990989238E-4	0.0
19	6.949173743241928E-4	0.0	0.00996048236531343	9.265564990989238E-4	0.0
20	6.949173743241928E-4	0.0	0.010423760614862892	0.0011581956238736547	0.0
21	6.949173743241928E-4	0.0	0.010887038864412354	0.0011581956238736547	0.0
22	6.949173743241928E-4	0.0	0.010887038864412354	0.0013898347486483856	0.0
23	6.949173743241928E-4	0.0	0.011118677989187085	0.0013898347486483856	0.0
24	9.265564990989238E-4	0.0	0.011350317113961816	0.0018531129981978476	0.0
25	9.265564990989238E-4	0.0	0.011350317113961816	0.0020847521229725783	0.0
26	9.265564990989238E-4	0.0	0.011350317113961816	0.0020847521229725783	0.0
27	9.265564990989238E-4	0.0	0.011350317113961816	0.0032429477468462332	0.0
28	9.265564990989238E-4	0.0	0.011350317113961816	0.008570647616665044	0.0
29	9.265564990989238E-4	0.0	0.011581956238736547	0.02015260385540159	0.0
30	9.265564990989238E-4	0.0	0.011581956238736547	0.0449379902062978	0.0
31	9.265564990989238E-4	0.0	0.011581956238736547	0.10516416264772785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAA	30	3.5957884E-4	30.833334	21
AGTAACG	25	0.005492588	29.6	23
TTGCCGA	45	4.000638E-6	28.777779	10
CATTCCG	35	8.86039E-4	26.42857	36
GGTATCA	255	0.0	25.392157	1
CGAGTTC	45	1.321343E-4	24.666668	14
GCCGAGT	45	1.321343E-4	24.666668	12
CGTCGGG	40	0.0019292452	23.125	28
CGAAGGT	40	0.0019292452	23.125	4
CGGACTC	40	0.0019292452	23.125	14
CCGAGTT	50	2.6983157E-4	22.2	13
TCGCCAT	110	7.4578566E-11	21.863636	13
TAGGACC	85	5.161928E-8	21.764706	4
AACGGCC	80	6.9394264E-7	20.8125	37
GCCGGCA	45	0.0038219967	20.555557	15
GTCGGGG	45	0.0038219967	20.555557	29
CGAAACA	55	5.135946E-4	20.181818	17
ACCTACT	55	5.135946E-4	20.181818	7
TAGTCCG	55	5.135946E-4	20.181818	16
TGCCGAG	55	5.135946E-4	20.181818	11
>>END_MODULE