FastQCFastQC Report
Fri 10 Feb 2017
ERR1632946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342872
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51861.512517790895728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40561.1829487388879816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20430.5958491798688723No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11930.347943255792249No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11580.33773536480085864No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.3354021325742551No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11000.32081943115798317No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8420.24557269185001984No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7340.21407405679087238No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6940.20240789565785486No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6740.19657481509134603No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6610.19278331272311533No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6060.17674234116521617No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6010.17528407102358898No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5990.1747007629669381No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5930.17295083879698547No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5830.17003429851373109No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5690.16595114211717493No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5650.16478452600387317No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5630.16420121794722228No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5590.16303460183392054No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5430.1583681373807135No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5420.15807648335238808No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.15603490515410998No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5280.1539933269558319No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5220.15224340278587928No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5190.15136844070090294No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5120.1493268625026249No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5050.14728528430434681No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4960.14466039804941785No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4920.1434937819361161No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4880.14232716582281435No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4850.141452203737838No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4810.14028558762453627No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4780.13941062553955996No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4730.13795235539793277No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4660.1359107771996547No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4650.13561912317132926No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4620.13474416108635293No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4600.13416085302970204No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4570.13328589094472573No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4510.13153596677477308No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4460.1300776966331459No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT4400.12832777246319327No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4340.12657784829324062No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4270.12453627009496254No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4250.12395296203831167No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4100.11957815161343008No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4020.11724491938682657No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4010.11695326535850112No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT3980.11607830327352482No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3950.1152033411885485No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3880.1131617629902704No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3840.11199514687696865No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3790.11053687673534147No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT3770.1099535686786906No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3750.1093702606220397No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3740.10907860659371427No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC3730.10878695256538883No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3730.10878695256538883No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3680.10732868242376165No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC3670.10703702839543619No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.10557875825380901No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC3610.10528710422548357No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3610.10528710422548357No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG3570.1041204881121818No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG3560.10382883408385636No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.10266221797055461No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC3520.10266221797055461No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3510.10237056394222918No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA3480.10149560185725286No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA3460.10091229380060197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCC200.001840135337.04
GAATCAA554.984031E-1033.6363641
CCAAGCA658.731149E-1131.30769236
ACTAGTG303.5942582E-430.8333328
ATAGACC250.00549103129.63
GCCTAGT250.00549103129.61
GTATAGA250.00549103129.61
TATAGAC250.00549103129.62
CCTAGTT358.856652E-426.4285722
GAACTTT358.856652E-426.4285721
CTAGTTC358.856652E-426.4285723
AAGTCTG358.856652E-426.42857237
GGTATCA7950.026.0628931
CAAGCAT809.731593E-1025.437537
GGGACTC451.3205683E-424.66666618
GTATCAA22650.024.0949231
TCCCTTG551.8976143E-523.54545431
ACTATAT400.001928439223.1251
TAGCGAA400.001928439223.12510
TGCCTTC400.001928439223.12512
Produced by FastQC (version 0.11.5)