##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1632946.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 342872 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.730616673277492 33.0 33.0 33.0 27.0 33.0 2 31.21871427238153 33.0 33.0 33.0 27.0 33.0 3 31.641224713595744 33.0 33.0 33.0 27.0 33.0 4 31.76212405795749 33.0 33.0 33.0 27.0 33.0 5 31.90661821321076 33.0 33.0 33.0 33.0 33.0 6 34.71508609626916 37.0 37.0 37.0 27.0 37.0 7 34.92654693296624 37.0 37.0 37.0 27.0 37.0 8 35.10289262465293 37.0 37.0 37.0 33.0 37.0 9 35.21058879115238 37.0 37.0 37.0 33.0 37.0 10 35.19576984997317 37.0 37.0 37.0 33.0 37.0 11 35.228423434984485 37.0 37.0 37.0 33.0 37.0 12 35.158341888518166 37.0 37.0 37.0 33.0 37.0 13 35.1951019622483 37.0 37.0 37.0 33.0 37.0 14 35.179355561259015 37.0 37.0 37.0 33.0 37.0 15 35.1961810821531 37.0 37.0 37.0 33.0 37.0 16 35.222794512237805 37.0 37.0 37.0 33.0 37.0 17 35.19768310039898 37.0 37.0 37.0 33.0 37.0 18 35.19739436291094 37.0 37.0 37.0 33.0 37.0 19 35.191115051681095 37.0 37.0 37.0 33.0 37.0 20 35.185077813294754 37.0 37.0 37.0 33.0 37.0 21 35.18234793158963 37.0 37.0 37.0 33.0 37.0 22 35.168701439604284 37.0 37.0 37.0 33.0 37.0 23 35.160091812688115 37.0 37.0 37.0 33.0 37.0 24 35.15194883221727 37.0 37.0 37.0 33.0 37.0 25 35.15625947875592 37.0 37.0 37.0 33.0 37.0 26 35.106975781049485 37.0 37.0 37.0 33.0 37.0 27 35.057689166802774 37.0 37.0 37.0 27.0 37.0 28 35.00008166312793 37.0 37.0 37.0 27.0 37.0 29 35.03979910870529 37.0 37.0 37.0 27.0 37.0 30 34.98129622716349 37.0 37.0 37.0 27.0 37.0 31 34.9335728785086 37.0 37.0 37.0 27.0 37.0 32 34.96525525560559 37.0 37.0 37.0 27.0 37.0 33 34.921480902494224 37.0 37.0 37.0 27.0 37.0 34 34.85097645768683 37.0 37.0 37.0 27.0 37.0 35 34.79178818917847 37.0 37.0 37.0 27.0 37.0 36 34.776415105345436 37.0 37.0 37.0 27.0 37.0 37 34.744464406542384 37.0 37.0 37.0 27.0 37.0 38 34.556309643248795 37.0 37.0 37.0 27.0 37.0 39 34.58787827527474 37.0 37.0 37.0 27.0 37.0 40 34.572047294617235 37.0 37.0 37.0 27.0 37.0 41 34.546195081546465 37.0 37.0 37.0 27.0 37.0 42 34.390615156676546 37.0 37.0 37.0 27.0 37.0 43 34.10635747450944 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 8.0 16 18.0 17 22.0 18 18.0 19 14.0 20 29.0 21 73.0 22 204.0 23 456.0 24 1047.0 25 1916.0 26 2981.0 27 4520.0 28 6626.0 29 8966.0 30 11755.0 31 14891.0 32 18761.0 33 23827.0 34 33725.0 35 58399.0 36 154616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6046921300077 19.18471032921907 13.573870132294266 22.636727408478965 2 17.263585244639398 23.954711962481625 37.22555355934576 21.556149233533215 3 19.274539769943303 28.267400079329896 29.918161879651883 22.53989827107492 4 12.068935346135001 18.16596280827831 40.01784922653352 29.74725261905317 5 14.447082293100632 36.55008282974405 33.14210550876129 15.860729368394036 6 26.6945099045708 41.76689843440118 16.270211624162954 15.26838003686507 7 26.02370563942229 32.96973797802095 21.48936046104669 19.517195921510066 8 25.20969924636599 32.78862082643085 19.79514221050421 22.206537716698943 9 27.97603770503278 13.00193658274808 19.26491518700856 39.75711052521058 10 17.55378100282321 26.10041064887188 30.87828694089923 25.46752140740568 11 35.288679157236515 24.044249749177535 21.906717375580392 18.760353718005554 12 24.666347791595697 26.288819151170117 27.74650598474066 21.298327072493525 13 30.616381623462985 22.564688863482584 24.59139270631606 22.227536806738375 14 23.574395109545254 21.24291280711169 28.75796215497328 26.42472992836977 15 26.711425838213675 25.973541146550318 26.72134207517674 20.593690940059265 16 21.430154693296625 27.617303250192492 25.728260108728623 25.224281947782263 17 22.444235749784173 25.28669590984391 25.37506708042652 26.894001259945405 18 24.46714809024942 23.603268858349473 28.731129984367342 23.19845306703376 19 27.142782146107002 23.81121818054551 28.587052894374576 20.458946778972912 20 26.25469562985604 22.667642735481465 29.29548052917707 21.782181105485428 21 23.532105275438063 25.464021559065774 28.94637065727152 22.057502508224644 22 23.123497981754124 25.75917543573112 28.358979444224087 22.758347138290674 23 23.05204274481439 25.37798362070977 29.918453533680207 21.651520100795633 24 23.315697986420588 25.63580578174946 27.738922980004197 23.309573251825753 25 23.321822721015423 25.868254042324835 29.863914230383347 20.946009006276395 26 23.63885064980518 25.857462843276792 28.58880281854453 21.914883688373504 27 23.98066917100259 25.219323829300734 29.010242889474792 21.789764110221892 28 22.24036958398469 25.7967988053851 29.184068690356753 22.778762920273454 29 24.162077976621013 25.839963601577264 27.42422828344105 22.57373013836067 30 23.787885858279477 24.778634592500993 29.91699526353858 21.516484285680953 31 23.399694346578315 24.963834900487644 28.748629226066868 22.88784152686717 32 20.761100352318067 25.146701976247698 29.544261415338667 24.54793625609557 33 21.525817214587367 25.2018245876012 30.400849296530485 22.871508901280947 34 21.62614620033132 25.513602743881098 29.752794045591358 23.107457010196224 35 22.33457383513381 26.394981217480574 28.609510254555637 22.66093469282998 36 23.058750787465875 25.824797592104343 28.961828320772764 22.154623299657015 37 23.1094985883945 25.769674980750835 28.411185515294335 22.709640915560325 38 22.584229683380386 25.515061014022727 28.029410392216334 23.87129891038055 39 21.96767306750041 25.365150843463454 28.96736974731095 23.69980634172519 40 22.113791735691454 26.908000653305024 29.036200097995753 21.94200751300777 41 22.84496838470333 24.852131407639003 28.759128771086583 23.54377143657108 42 21.405364100888963 26.859586084603 29.088114515037685 22.646935299470357 43 22.64985183975361 25.499603350521475 28.523472316199634 23.32707249352528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6.0 1 31.0 2 56.0 3 273.0 4 490.0 5 490.0 6 694.5 7 899.0 8 868.0 9 837.0 10 1109.0 11 1381.0 12 1381.0 13 2205.5 14 3030.0 15 4993.0 16 6956.0 17 7289.5 18 7623.0 19 7623.0 20 5809.5 21 3996.0 22 2799.0 23 1602.0 24 1587.0 25 1572.0 26 1572.0 27 1850.0 28 2128.0 29 2718.0 30 3308.0 31 4805.0 32 6302.0 33 6302.0 34 8734.5 35 11167.0 36 11950.0 37 12733.0 38 14866.5 39 17000.0 40 17000.0 41 19640.0 42 22280.0 43 24657.0 44 27034.0 45 28400.0 46 29766.0 47 29766.0 48 31731.5 49 33697.0 50 34226.0 51 34755.0 52 33878.5 53 33002.0 54 33002.0 55 30096.5 56 27191.0 57 23150.0 58 19109.0 59 16560.0 60 14011.0 61 14011.0 62 11393.0 63 8775.0 64 6551.0 65 4327.0 66 3924.5 67 3522.0 68 3522.0 69 2896.5 70 2271.0 71 1654.5 72 1038.0 73 897.5 74 757.0 75 757.0 76 449.5 77 142.0 78 104.0 79 66.0 80 47.0 81 28.0 82 28.0 83 18.5 84 9.0 85 6.0 86 3.0 87 3.0 88 3.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 342872.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.848713222427026 #Duplication Level Percentage of deduplicated Percentage of total 1 74.7100239202058 19.311579831540634 2 9.520693234643678 4.921953382020113 3 3.8847768199666017 3.0124944585734617 4 2.331091754298867 2.41022889008143 5 1.5536850656677348 2.008037985020649 6 1.0764092611815679 1.669427658134814 7 0.8349505799521597 1.5107678667257751 8 0.6521641016383084 1.348608226976831 9 0.5201516450783048 1.2100725635222473 >10 3.8419009793744645 20.099337362047645 >50 0.5664124204540326 10.431006323059334 >100 0.47501918129710696 23.62747614270048 >500 0.024822855079658798 3.8057934156186564 >1k 0.0067698695671796726 3.1206981030822 >5k 0.0011283115945299454 1.512517790895728 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 5186 1.512517790895728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4056 1.1829487388879816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2043 0.5958491798688723 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 1193 0.347943255792249 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 1158 0.33773536480085864 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1150 0.3354021325742551 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 1100 0.32081943115798317 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 842 0.24557269185001984 No Hit GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG 734 0.21407405679087238 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 694 0.20240789565785486 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 674 0.19657481509134603 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 661 0.19278331272311533 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 606 0.17674234116521617 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 601 0.17528407102358898 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 599 0.1747007629669381 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 593 0.17295083879698547 No Hit GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC 583 0.17003429851373109 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA 569 0.16595114211717493 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 565 0.16478452600387317 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC 563 0.16420121794722228 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 559 0.16303460183392054 No Hit GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA 543 0.1583681373807135 No Hit GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC 542 0.15807648335238808 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 535 0.15603490515410998 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 528 0.1539933269558319 No Hit TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG 522 0.15224340278587928 No Hit GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG 519 0.15136844070090294 No Hit GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG 512 0.1493268625026249 No Hit GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT 505 0.14728528430434681 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA 496 0.14466039804941785 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 492 0.1434937819361161 No Hit CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT 488 0.14232716582281435 No Hit CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 485 0.141452203737838 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 481 0.14028558762453627 No Hit ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT 478 0.13941062553955996 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 473 0.13795235539793277 No Hit GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC 466 0.1359107771996547 No Hit AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC 465 0.13561912317132926 No Hit ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT 462 0.13474416108635293 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 460 0.13416085302970204 No Hit GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA 457 0.13328589094472573 No Hit CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG 451 0.13153596677477308 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 446 0.1300776966331459 No Hit CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT 440 0.12832777246319327 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 434 0.12657784829324062 No Hit GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC 427 0.12453627009496254 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 425 0.12395296203831167 No Hit CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA 410 0.11957815161343008 No Hit CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG 402 0.11724491938682657 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 401 0.11695326535850112 No Hit ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT 398 0.11607830327352482 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 395 0.1152033411885485 No Hit TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG 388 0.1131617629902704 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 384 0.11199514687696865 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 379 0.11053687673534147 No Hit GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT 377 0.1099535686786906 No Hit GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC 375 0.1093702606220397 No Hit GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA 374 0.10907860659371427 No Hit ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC 373 0.10878695256538883 No Hit CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT 373 0.10878695256538883 No Hit GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG 368 0.10732868242376165 No Hit GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC 367 0.10703702839543619 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 362 0.10557875825380901 No Hit ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC 361 0.10528710422548357 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 361 0.10528710422548357 No Hit GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG 357 0.1041204881121818 No Hit CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG 356 0.10382883408385636 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 352 0.10266221797055461 No Hit GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC 352 0.10266221797055461 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 351 0.10237056394222918 No Hit GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA 348 0.10149560185725286 No Hit ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA 346 0.10091229380060197 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.002333232226603514 0.0 0.0 2 2.9165402832543924E-4 0.0 0.0026248862549289533 0.0 0.0 3 2.9165402832543924E-4 0.0 0.004374810424881588 0.0 0.0 4 2.9165402832543924E-4 0.0 0.005833080566508784 0.0 0.0 5 5.833080566508785E-4 0.0 0.009041274878088616 0.0 0.0 6 5.833080566508785E-4 0.0 0.017499241699526352 0.0 0.0 7 5.833080566508785E-4 0.0 0.03033201894584568 0.0 0.0 8 5.833080566508785E-4 0.0 0.03441517534240183 0.0 0.0 9 8.749620849763177E-4 0.0 0.04083156396556149 5.833080566508785E-4 0.0 10 0.004374810424881588 0.0 0.047831260645372034 8.749620849763177E-4 0.0 11 0.004666464453207028 0.0 0.07495508527963789 0.001166616113301757 0.0 12 0.004958118481532467 0.0 0.08137147390279754 0.001166616113301757 0.0 13 0.004958118481532467 0.0 0.0877878625259572 0.001166616113301757 0.0 14 0.004958118481532467 0.0 0.09187101892251336 0.001166616113301757 0.0 15 0.004958118481532467 0.0 0.10266221797055461 0.001166616113301757 0.0 16 0.004958118481532467 0.0 0.11287010896194498 0.001166616113301757 0.0 17 0.004958118481532467 0.0 0.11695326535850113 0.001458270141627196 0.0 18 0.0055414265381833454 0.0 0.1204531136984064 0.001458270141627196 0.0 19 0.0055414265381833454 0.0 0.12278634592500992 0.0017499241699526354 0.0 20 0.0055414265381833454 0.0 0.12599454023658974 0.0020415781982780746 0.0 21 0.005833080566508784 0.0 0.1286194264915187 0.0026248862549289533 0.0 22 0.005833080566508784 0.0 0.13036935066147134 0.004083156396556149 0.0 23 0.005833080566508784 0.0 0.13211927483142397 0.005833080566508784 0.0 24 0.005833080566508784 0.0 0.13270258288807485 0.006999696679810542 0.0 25 0.005833080566508784 0.0 0.13270258288807485 0.007291350708135981 0.0 26 0.005833080566508784 0.0 0.13299423691640028 0.008166312793112298 0.0 27 0.005833080566508784 0.0 0.13299423691640028 0.011957815161343009 0.0 28 0.005833080566508784 0.0 0.13299423691640028 0.032956905200774635 0.0 29 0.005833080566508784 0.0 0.13328589094472573 0.09245432697916424 0.0 30 0.005833080566508784 0.0 0.13328589094472573 0.16624279614550036 0.0 31 0.005833080566508784 0.0 0.13357754497305116 0.31556965864812525 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCC 20 0.0018401353 37.0 4 GAATCAA 55 4.984031E-10 33.636364 1 CCAAGCA 65 8.731149E-11 31.307692 36 ACTAGTG 30 3.5942582E-4 30.833332 8 ATAGACC 25 0.005491031 29.6 3 GCCTAGT 25 0.005491031 29.6 1 GTATAGA 25 0.005491031 29.6 1 TATAGAC 25 0.005491031 29.6 2 CCTAGTT 35 8.856652E-4 26.428572 2 GAACTTT 35 8.856652E-4 26.428572 1 CTAGTTC 35 8.856652E-4 26.428572 3 AAGTCTG 35 8.856652E-4 26.428572 37 GGTATCA 795 0.0 26.062893 1 CAAGCAT 80 9.731593E-10 25.4375 37 GGGACTC 45 1.3205683E-4 24.666666 18 GTATCAA 2265 0.0 24.094923 1 TCCCTTG 55 1.8976143E-5 23.545454 31 ACTATAT 40 0.0019284392 23.125 1 TAGCGAA 40 0.0019284392 23.125 10 TGCCTTC 40 0.0019284392 23.125 12 >>END_MODULE