FastQCFastQC Report
Fri 10 Feb 2017
ERR1632945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399279
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45141.1305377943743598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36330.9098900768635466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17160.42977466884058513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.23342074088544604No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7770.19460076788411113No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7540.18884038479359044No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7270.1820781959481966No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7210.18057548731588688No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7090.17757007005126743No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6960.17431420134792966No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6920.17331239559305647No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6860.17180968696074675No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6850.17155923552202845No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6650.16655020674766266No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6510.1630438866056066No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6320.1582853092699591No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6270.15703305207636764No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6270.15703305207636764No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6250.15653214919893108No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5760.14426002870173488No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5690.14250686863070686No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5540.13875009704993252No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5260.13173745676582038No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5200.13023474813351066No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5190.12998429669479236No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5180.12973384525607407No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5150.1289824909399192No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5140.12873203950120093No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.12798068518504604No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5110.12798068518504604No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5010.12547617079786316No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5000.12522571935914487No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4940.12372301072683513No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4860.12171939921708881No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4780.11971578770734248No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4590.11495721037169497No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT4460.11170134166835723No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4450.11145089022963893No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4430.11094998735220235No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4260.1066923128939914No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4190.10493915282296339No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4130.10343644419065366No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4070.10193373555834391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT507.3068804E-933.37
CGTTATT352.384434E-531.71428510
TGTTACG352.384434E-531.71428516
GGTATCA7450.028.8053681
TCAATTC453.9996485E-628.77777727
GGACCGT652.9776857E-928.4615386
TTGTTAC405.9305712E-527.7515
CCGGGGC604.3079126E-827.74999834
TACCGGG556.244554E-726.9090932
ATGCCTT358.859219E-426.428579
CAATTCA509.0717E-625.89999828
CCGTCAA509.0717E-625.89999824
AGGACCG659.359792E-825.6153855
CGCCGTT659.359792E-825.61538525
CCTTAGG751.3680619E-824.6666661
TTTACCG601.3337576E-624.66666630
TTACCGG601.3337576E-624.66666631
GCCGCCG851.9663275E-923.94117723
GTATCAA21200.023.8231141
TACGGCC701.9163963E-723.78571319
Produced by FastQC (version 0.11.5)