##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632945.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399279
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73074717177713	33.0	33.0	33.0	27.0	33.0
2	31.203824894372104	33.0	33.0	33.0	27.0	33.0
3	31.653858580090613	33.0	33.0	33.0	27.0	33.0
4	31.781994545167663	33.0	33.0	33.0	27.0	33.0
5	31.908034231702644	33.0	33.0	33.0	33.0	33.0
6	34.71939420806003	37.0	37.0	37.0	27.0	37.0
7	34.927992706854106	37.0	37.0	37.0	27.0	37.0
8	35.09961455523581	37.0	37.0	37.0	33.0	37.0
9	35.17907778771235	37.0	37.0	37.0	33.0	37.0
10	35.17673105773156	37.0	37.0	37.0	33.0	37.0
11	35.228444270798114	37.0	37.0	37.0	33.0	37.0
12	35.160612003135654	37.0	37.0	37.0	33.0	37.0
13	35.19881837011213	37.0	37.0	37.0	33.0	37.0
14	35.172698789568194	37.0	37.0	37.0	33.0	37.0
15	35.19408483792035	37.0	37.0	37.0	33.0	37.0
16	35.189726982886654	37.0	37.0	37.0	33.0	37.0
17	35.17251095098916	37.0	37.0	37.0	33.0	37.0
18	35.17822374830632	37.0	37.0	37.0	33.0	37.0
19	35.185206334417785	37.0	37.0	37.0	33.0	37.0
20	35.173031889981694	37.0	37.0	37.0	33.0	37.0
21	35.18523137956166	37.0	37.0	37.0	33.0	37.0
22	35.18529148790695	37.0	37.0	37.0	33.0	37.0
23	35.18282955026435	37.0	37.0	37.0	33.0	37.0
24	35.162510425041134	37.0	37.0	37.0	33.0	37.0
25	35.16889443221407	37.0	37.0	37.0	33.0	37.0
26	35.12840394811648	37.0	37.0	37.0	33.0	37.0
27	35.0632515108483	37.0	37.0	37.0	27.0	37.0
28	35.02799796633432	37.0	37.0	37.0	27.0	37.0
29	35.05925931491513	37.0	37.0	37.0	27.0	37.0
30	35.009259189689416	37.0	37.0	37.0	27.0	37.0
31	34.9760242837715	37.0	37.0	37.0	27.0	37.0
32	34.977126270101856	37.0	37.0	37.0	27.0	37.0
33	34.96120507214254	37.0	37.0	37.0	27.0	37.0
34	34.888316189932354	37.0	37.0	37.0	27.0	37.0
35	34.86612619246191	37.0	37.0	37.0	27.0	37.0
36	34.815259505258226	37.0	37.0	37.0	27.0	37.0
37	34.82213439725105	37.0	37.0	37.0	27.0	37.0
38	34.64192707355007	37.0	37.0	37.0	27.0	37.0
39	34.71097653520471	37.0	37.0	37.0	27.0	37.0
40	34.699465787081216	37.0	37.0	37.0	27.0	37.0
41	34.68590885070339	37.0	37.0	37.0	27.0	37.0
42	34.56247886815986	37.0	37.0	37.0	27.0	37.0
43	34.33259199707473	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	18.0
17	18.0
18	16.0
19	12.0
20	37.0
21	82.0
22	251.0
23	568.0
24	1194.0
25	2154.0
26	3556.0
27	5355.0
28	7467.0
29	10040.0
30	13233.0
31	17013.0
32	21453.0
33	27492.0
34	38674.0
35	68390.0
36	182246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81417254601419	20.171358874371055	13.789856215829033	23.224612363785724
2	16.630476433771875	23.708985446266894	38.09867285782623	21.561865262135
3	17.476000490884818	28.66366625843082	30.401047888819598	23.45928536186476
4	11.65500815219433	18.17726451929603	40.90473077722595	29.26299655128369
5	13.301976813205805	37.26316685826202	34.86158801239234	14.573268316139842
6	26.79905529717315	42.41420159838108	16.542567978781754	14.244175125664011
7	25.896428312032437	32.892037898311706	23.14772377209921	18.063810017556644
8	24.39797735418091	34.243974764513034	20.247496111741413	21.11055176956464
9	27.017699403174223	13.884025956787108	20.133039804247154	38.96523483579151
10	16.470938867308323	27.04149228985246	32.77307346492052	23.7144953779187
11	33.72128261190797	24.638661186789186	23.136453457356886	18.50360274394596
12	23.783870426443666	27.474272375957664	28.150992163374482	20.59086503422419
13	28.64162653182361	22.975413182261025	25.4836843410247	22.899275944890665
14	22.96013564449921	21.217995436774785	28.770609022763534	27.05125989596247
15	25.07619985023004	27.515095960468745	26.52280736026688	20.885896829034333
16	21.82258520984074	28.35235512010399	25.89066792894192	23.934391741113355
17	22.019690492112034	26.225271051069555	26.030920734624157	25.724117722194258
18	23.453024075896803	25.643973261804405	28.73279085551707	22.170211806781722
19	26.28212352765861	24.501413798371566	28.812434413029486	20.404028260940347
20	25.080207073249532	24.663706330661018	29.224427029721074	21.03165956636838
21	22.622527105106954	26.83086262989038	28.979235071215864	21.567375193786802
22	22.393614490118438	26.54184166960947	28.83647775114644	22.228066089125647
23	22.16019374923299	26.55987417319719	30.07571146992454	21.20422060764528
24	22.61927123640362	27.203033467825755	27.20603888509038	22.971656410680254
25	22.69691118240629	26.971616338450055	29.50117586950478	20.830296609638875
26	22.483025653740867	26.975623561469547	29.027822650327217	21.51352813446237
27	22.640058705817236	26.65479526847142	28.39468141324738	22.310464612463964
28	21.70337032501083	26.28162262478117	29.87484941607247	22.14015763413553
29	23.833710262748603	26.516295622860202	28.031526827105857	21.61846728728533
30	23.35584891767411	25.792490964964347	29.339634691531487	21.512025425830057
31	22.814122455726444	26.30616686577556	28.793400103686896	22.086310574811097
32	20.734624160048487	25.94601769689866	29.681250453943232	23.63810768910962
33	20.936738471094145	26.120582349685307	30.44587869635017	22.496800482870373
34	22.217046225822042	25.723115916439383	30.014100415999838	22.045737441738734
35	22.120622421915503	27.12088539592616	28.601053398751247	22.15743878340709
36	23.011227737997743	26.952331577668748	28.194069810834026	21.842370873499483
37	22.181732572962765	26.209743061869016	28.736046724220408	22.872477640947807
38	22.230069700635397	26.5258127775315	28.274715174101317	22.969402347731787
39	21.414349364729926	26.359262570783837	29.28002724911653	22.946360815369705
40	21.503259625474918	27.42919111698837	29.583323941404384	21.48422531613233
41	22.227064283370776	25.554812549620692	29.04936147405699	23.168761692951545
42	20.96704309517906	27.669374046719213	28.951685412956856	22.411897445144874
43	22.47976978503753	25.596387488447924	28.932150200736828	22.991692525777715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	71.5
2	79.0
3	290.5
4	502.0
5	502.0
6	656.5
7	811.0
8	783.5
9	756.0
10	1021.0
11	1286.0
12	1286.0
13	1882.0
14	2478.0
15	4289.5
16	6101.0
17	6324.0
18	6547.0
19	6547.0
20	5036.5
21	3526.0
22	2709.5
23	1893.0
24	2165.0
25	2437.0
26	2437.0
27	3143.5
28	3850.0
29	5256.5
30	6663.0
31	8935.5
32	11208.0
33	11208.0
34	14559.5
35	17911.0
36	20342.0
37	22773.0
38	25444.5
39	28116.0
40	28116.0
41	30701.5
42	33287.0
43	35267.5
44	37248.0
45	36204.5
46	35161.0
47	35161.0
48	34829.0
49	34497.0
50	34252.5
51	34008.0
52	31926.5
53	29845.0
54	29845.0
55	28194.5
56	26544.0
57	21819.0
58	17094.0
59	15590.5
60	14087.0
61	14087.0
62	11588.5
63	9090.0
64	6657.5
65	4225.0
66	3662.5
67	3100.0
68	3100.0
69	2433.0
70	1766.0
71	1391.5
72	1017.0
73	924.0
74	831.0
75	831.0
76	527.5
77	224.0
78	157.5
79	91.0
80	75.5
81	60.0
82	60.0
83	53.5
84	47.0
85	34.5
86	22.0
87	22.5
88	23.0
89	23.0
90	16.0
91	9.0
92	5.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	399279.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.818160014546244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.94899932016381	25.756482133863667
2	7.101534516367527	4.519155231812104
3	2.7618485294366124	2.636308153366601
4	1.5779629511011786	2.008315107006516
5	1.1117183718125496	1.7686416522721258
6	0.7617419949466377	1.4542337211007075
7	0.5965280713057347	1.328629793818216
8	0.5171190012703026	1.3163020103184664
9	0.42359131362987934	1.2130106578062607
>10	3.3989303732405793	21.873591523320105
>50	0.45109496027528695	10.127374981486122
>100	0.32456832507612116	19.145767270127852
>500	0.022004632208550586	4.385920277900956
>1k	0.002357639165201848	2.466267485800292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4514	1.1305377943743598	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3633	0.9098900768635466	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1716	0.42977466884058513	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	932	0.23342074088544604	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	777	0.19460076788411113	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	754	0.18884038479359044	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	727	0.1820781959481966	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	721	0.18057548731588688	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	709	0.17757007005126743	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	696	0.17431420134792966	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	692	0.17331239559305647	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	686	0.17180968696074675	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	685	0.17155923552202845	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	665	0.16655020674766266	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	651	0.1630438866056066	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	632	0.1582853092699591	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	627	0.15703305207636764	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	627	0.15703305207636764	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	625	0.15653214919893108	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	576	0.14426002870173488	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	569	0.14250686863070686	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	554	0.13875009704993252	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	526	0.13173745676582038	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	520	0.13023474813351066	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	519	0.12998429669479236	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	518	0.12973384525607407	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	515	0.1289824909399192	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	514	0.12873203950120093	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.12798068518504604	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	511	0.12798068518504604	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	501	0.12547617079786316	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	500	0.12522571935914487	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	494	0.12372301072683513	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	486	0.12171939921708881	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	478	0.11971578770734248	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	459	0.11495721037169497	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	446	0.11170134166835723	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	445	0.11145089022963893	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	443	0.11094998735220235	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	426	0.1066923128939914	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	419	0.10493915282296339	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	413	0.10343644419065366	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	407	0.10193373555834391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.002504514387182897	0.0	0.0
2	2.5045143871828973E-4	0.0	0.0027549658259011867	5.009028774365795E-4	0.0
3	2.5045143871828973E-4	0.0	0.0030054172646194767	5.009028774365795E-4	0.0
4	2.5045143871828973E-4	0.0	0.003506320142056056	5.009028774365795E-4	0.0
5	2.5045143871828973E-4	0.0	0.0060108345292389535	5.009028774365795E-4	0.0
6	2.5045143871828973E-4	0.0	0.009517154671295009	5.009028774365795E-4	0.0
7	2.5045143871828973E-4	0.0	0.019284760781308308	5.009028774365795E-4	0.0
8	5.009028774365795E-4	0.0	0.023542435239519235	5.009028774365795E-4	0.0
9	7.513543161548692E-4	0.0	0.029052366891321606	0.0012522571935914485	0.0
10	0.0015027086323097384	0.0	0.03356049278825082	0.0012522571935914485	0.0
11	0.0022540629484646075	0.0	0.04783622479519334	0.0015027086323097384	0.0
12	0.0022540629484646075	0.0	0.0510920934985311	0.001753160071028028	0.0
13	0.0022540629484646075	0.0	0.05560021939546032	0.001753160071028028	0.0
14	0.0022540629484646075	0.0	0.06010834529238953	0.001753160071028028	0.0
15	0.0022540629484646075	0.0	0.06611917982162849	0.001753160071028028	0.0
16	0.0022540629484646075	0.0	0.07363272298317718	0.001753160071028028	0.0
17	0.0022540629484646075	0.0	0.07588678593164179	0.0022540629484646075	0.0
18	0.0022540629484646075	0.0	0.07814084888010639	0.002504514387182897	0.0
19	0.0022540629484646075	0.0	0.07939310607369784	0.0032558687033377664	0.0
20	0.0022540629484646075	0.0	0.08189762046088074	0.0032558687033377664	0.0
21	0.0022540629484646075	0.0	0.08415168340934534	0.0032558687033377664	0.0
22	0.0022540629484646075	0.0	0.08465258628678192	0.0037567715807743456	0.0
23	0.0022540629484646075	0.0	0.08615529491909166	0.005009028774365794	0.0
24	0.0022540629484646075	0.0	0.08690664923524653	0.006762188845393822	0.0
25	0.0022540629484646075	0.0	0.08690664923524653	0.00876580035514014	0.0
26	0.0022540629484646075	0.0	0.08740755211268311	0.010518960426168168	0.0
27	0.0022540629484646075	0.0	0.08740755211268311	0.013023474813351065	0.0
28	0.0022540629484646075	0.0	0.08740755211268311	0.03381094422696911	0.0
29	0.0022540629484646075	0.0	0.0876580035514014	0.0851534891642185	0.0
30	0.0022540629484646075	0.0	0.0876580035514014	0.1460131887727629	0.0
31	0.0022540629484646075	0.0	0.0876580035514014	0.2847632858226954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	50	7.3068804E-9	33.3	7
CGTTATT	35	2.384434E-5	31.714285	10
TGTTACG	35	2.384434E-5	31.714285	16
GGTATCA	745	0.0	28.805368	1
TCAATTC	45	3.9996485E-6	28.777777	27
GGACCGT	65	2.9776857E-9	28.461538	6
TTGTTAC	40	5.9305712E-5	27.75	15
CCGGGGC	60	4.3079126E-8	27.749998	34
TACCGGG	55	6.244554E-7	26.90909	32
ATGCCTT	35	8.859219E-4	26.42857	9
CAATTCA	50	9.0717E-6	25.899998	28
CCGTCAA	50	9.0717E-6	25.899998	24
AGGACCG	65	9.359792E-8	25.615385	5
CGCCGTT	65	9.359792E-8	25.615385	25
CCTTAGG	75	1.3680619E-8	24.666666	1
TTTACCG	60	1.3337576E-6	24.666666	30
TTACCGG	60	1.3337576E-6	24.666666	31
GCCGCCG	85	1.9663275E-9	23.941177	23
GTATCAA	2120	0.0	23.823114	1
TACGGCC	70	1.9163963E-7	23.785713	19
>>END_MODULE