FastQCFastQC Report
Fri 10 Feb 2017
ERR1632944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500898
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9380.1872636744407045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.14094685944044497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2150.030.9767441
TTAACGG1103.6379788E-1223.54545435
TAACGGC1155.456968E-1222.5217436
GTATCAA6100.021.8360651
ATTCGGA656.892197E-519.92307736
GTGTATA759.248664E-619.7333341
TTTAACG1355.638867E-1119.18518634
AACGGCC1457.2759576E-1219.1379337
AGCTTCG609.226896E-418.521
TAGCCGG500.00702947618.530
CCGTCTA609.226896E-418.526
CGCTCAC801.613704E-518.510
ATTAGAG1653.6379788E-1217.9393963
GGCACCG1653.6379788E-1217.9393969
CCGTTAA1156.3835614E-817.69565216
TAGGGGT752.0643337E-417.2666664
TTAGGCT650.001578251817.0769234
TTTTTCG650.001578251817.07692325
GTTCGCC650.001578251817.07692326
AGCGCCC650.001578251817.07692310
Produced by FastQC (version 0.11.5)