##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632944.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500898
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.690082611629514	33.0	33.0	33.0	27.0	33.0
2	31.22374215908229	33.0	33.0	33.0	27.0	33.0
3	31.656019788459925	33.0	33.0	33.0	27.0	33.0
4	31.78931439135313	33.0	33.0	33.0	27.0	33.0
5	31.896569760709767	33.0	33.0	33.0	33.0	33.0
6	34.784427168804825	37.0	37.0	37.0	27.0	37.0
7	34.967763896042705	37.0	37.0	37.0	27.0	37.0
8	35.133356491740834	37.0	37.0	37.0	33.0	37.0
9	35.231663931578886	37.0	37.0	37.0	33.0	37.0
10	35.24800059093867	37.0	37.0	37.0	33.0	37.0
11	35.286842830276825	37.0	37.0	37.0	33.0	37.0
12	35.24525951391301	37.0	37.0	37.0	33.0	37.0
13	35.28250062887055	37.0	37.0	37.0	33.0	37.0
14	35.25579259649669	37.0	37.0	37.0	33.0	37.0
15	35.26561495553985	37.0	37.0	37.0	33.0	37.0
16	35.263091487688115	37.0	37.0	37.0	33.0	37.0
17	35.22643332574696	37.0	37.0	37.0	33.0	37.0
18	35.24095125155221	37.0	37.0	37.0	33.0	37.0
19	35.25677682881545	37.0	37.0	37.0	33.0	37.0
20	35.24830005310462	37.0	37.0	37.0	33.0	37.0
21	35.25498005581975	37.0	37.0	37.0	33.0	37.0
22	35.25047015560054	37.0	37.0	37.0	33.0	37.0
23	35.24811837939061	37.0	37.0	37.0	33.0	37.0
24	35.23749346174271	37.0	37.0	37.0	33.0	37.0
25	35.2318456052929	37.0	37.0	37.0	33.0	37.0
26	35.20448274898283	37.0	37.0	37.0	33.0	37.0
27	35.15226652931335	37.0	37.0	37.0	33.0	37.0
28	35.11984875164205	37.0	37.0	37.0	33.0	37.0
29	35.14828567892066	37.0	37.0	37.0	33.0	37.0
30	35.12010628910477	37.0	37.0	37.0	33.0	37.0
31	35.119233855994636	37.0	37.0	37.0	33.0	37.0
32	35.14468215085706	37.0	37.0	37.0	33.0	37.0
33	35.138499255337415	37.0	37.0	37.0	33.0	37.0
34	35.07754273325108	37.0	37.0	37.0	33.0	37.0
35	35.065015232642175	37.0	37.0	37.0	27.0	37.0
36	35.046416635722245	37.0	37.0	37.0	27.0	37.0
37	35.082803285299605	37.0	37.0	37.0	33.0	37.0
38	34.93449764223455	37.0	37.0	37.0	27.0	37.0
39	35.01471357442034	37.0	37.0	37.0	27.0	37.0
40	35.05771035220744	37.0	37.0	37.0	27.0	37.0
41	35.07758066512543	37.0	37.0	37.0	33.0	37.0
42	34.99200635658357	37.0	37.0	37.0	27.0	37.0
43	34.80410582593662	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	6.0
16	26.0
17	35.0
18	32.0
19	22.0
20	31.0
21	102.0
22	256.0
23	597.0
24	1249.0
25	2385.0
26	3950.0
27	6008.0
28	8816.0
29	11619.0
30	15347.0
31	19744.0
32	25602.0
33	32846.0
34	46350.0
35	84753.0
36	241119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78475058794405	20.469636532787113	13.119237848823511	25.62637503044532
2	16.041788947051096	22.73796261913603	37.455330226912466	23.764918206900408
3	17.78984943042296	26.441111763273163	30.11810787825066	25.650930928053217
4	12.860103254554819	17.7429336910908	38.961425280196764	30.435537774157613
5	13.927785696888389	36.88495462149979	35.69069950369137	13.496560177920456
6	30.161030788703492	39.58550443403647	17.298132553933137	12.955332223326904
7	26.82402405280117	31.74119281769941	23.87452135963809	17.56026176986133
8	24.547911950137554	36.198986620030425	20.66867905242185	18.58442237741017
9	25.863349424433718	15.253604526270816	20.144021337677533	38.73902471161794
10	14.77167008053536	28.131276227894702	34.44633438344733	22.65071930812261
11	34.523196339374486	23.226684873966356	24.144436591880982	18.10568219477818
12	22.613785640988784	25.432323546909753	30.778521774892294	21.175369037209173
13	28.076973755135775	21.805836717255808	26.218511553250362	23.898677974358055
14	21.92102983042456	20.576444705309267	27.522968748128363	29.979556716137818
15	23.636748399873827	28.848987218954758	23.932816661276348	23.581447719895067
16	23.40915715375186	28.100531445523842	25.038231336519612	23.45208006420469
17	22.507576392798534	27.703244972030234	26.38621036618234	23.402968268988896
18	23.08294303431038	27.49262324864543	27.20873311532488	22.215700601719313
19	24.48043314207683	26.68307719336072	26.832608634891734	22.003881029670712
20	24.0240528011691	26.627776513381967	26.869342660581598	22.47882802486734
21	23.30194969834178	27.061597371121465	26.819432299589934	22.817020630946818
22	22.91664171148617	26.893499275301558	26.899488518620558	23.290370494591713
23	23.07875056398708	26.704239186421187	27.317338060842726	22.89967218874901
24	23.24205726515179	27.216319490195605	26.224301155125396	23.31732208952721
25	23.421934206165727	26.690463926787487	27.144648211811585	22.742953655235198
26	23.35285826655327	27.122887294419222	26.74077357066708	22.783480868360424
27	23.277194159289916	27.082360081293995	26.341690324177776	23.29875543523831
28	22.9380033459906	26.76153628083961	27.23408757870864	23.06637279446115
29	23.90945861233225	26.79188178032254	26.51358160743305	22.785077999912158
30	23.529940227351677	26.747761021205914	26.72959364980495	22.99270510163746
31	23.431117712588193	26.544925314135813	26.922646926120684	23.10131004715531
32	22.666690623639944	26.56648659008421	27.05640669357833	23.710416092697514
33	22.848563979093548	26.693658189890957	27.366649497502486	23.091128333513012
34	23.34267655291097	26.299965262388753	27.238679331919872	23.118678852780405
35	23.044012952736885	26.98094222775895	26.95778382025881	23.017260999245355
36	23.733175217309714	26.80964986883557	26.544725672691843	22.91244924116287
37	23.1594057073496	26.270617970125652	27.003900993815105	23.566075328709637
38	23.328102727501406	26.801464569632937	26.631769342261297	23.238663360604356
39	22.747545408446427	26.745165682434347	27.173396579742782	23.33389232937644
40	22.938402628878535	26.842989989978	27.368246629054216	22.850360752089248
41	22.92682342512847	26.16640513637507	27.367248421834383	23.539523016662077
42	22.671881301183074	27.020071950776405	27.076969762306895	23.231076985733623
43	23.26441710687605	25.9957117017836	27.204141362113642	23.53572982922671
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	9.5
2	13.0
3	64.0
4	115.0
5	115.0
6	138.0
7	161.0
8	148.0
9	135.0
10	196.0
11	257.0
12	257.0
13	414.5
14	572.0
15	1001.5
16	1431.0
17	1683.0
18	1935.0
19	1935.0
20	2132.0
21	2329.0
22	2766.0
23	3203.0
24	4470.0
25	5737.0
26	5737.0
27	7642.5
28	9548.0
29	11873.5
30	14199.0
31	17335.5
32	20472.0
33	20472.0
34	23570.5
35	26669.0
36	29602.5
37	32536.0
38	34708.5
39	36881.0
40	36881.0
41	38545.5
42	40210.0
43	40784.5
44	41359.0
45	40782.5
46	40206.0
47	40206.0
48	38787.0
49	37368.0
50	36824.0
51	36280.0
52	34304.5
53	32329.0
54	32329.0
55	30934.0
56	29539.0
57	26169.0
58	22799.0
59	21248.5
60	19698.0
61	19698.0
62	17404.0
63	15110.0
64	12837.5
65	10565.0
66	8933.5
67	7302.0
68	7302.0
69	5992.0
70	4682.0
71	3842.5
72	3003.0
73	2430.5
74	1858.0
75	1858.0
76	1452.0
77	1046.0
78	810.0
79	574.0
80	459.0
81	344.0
82	344.0
83	273.0
84	202.0
85	170.5
86	139.0
87	96.5
88	54.0
89	54.0
90	40.0
91	26.0
92	16.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	500898.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.40610244187683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.7768380384764	62.504311534028766
2	6.638251226825023	9.347467918215143
3	1.8050779992736483	3.812655195973156
4	0.8090657898711613	2.278526755355479
5	0.44682946602738205	1.572976057958649
6	0.2805939922712494	1.1853317618654868
7	0.19950578879507253	0.9832497502557322
8	0.1478187188653834	0.8325871890610539
9	0.11673098944019085	0.7396716600600948
>10	0.6838072828144603	9.29618066806704
>50	0.06327378310936431	3.1362618638227695
>100	0.031636890173421604	3.9808791827663526
>500	5.700340571787589E-4	0.32990046257020994
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.1872636744407045	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	706	0.14094685944044497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.989243318999078E-4	0.0	0.0
2	3.992828879332718E-4	0.0	5.989243318999078E-4	0.0	0.0
3	3.992828879332718E-4	0.0	5.989243318999078E-4	0.0	0.0
4	3.992828879332718E-4	0.0	5.989243318999078E-4	0.0	0.0
5	3.992828879332718E-4	0.0	7.985657758665436E-4	0.0	0.0
6	3.992828879332718E-4	0.0	0.0011978486637998155	0.0	0.0
7	3.992828879332718E-4	0.0	0.0017967729956997233	1.996414439666359E-4	0.0
8	3.992828879332718E-4	0.0	0.002994621659499539	1.996414439666359E-4	0.0
9	3.992828879332718E-4	0.0	0.003992828879332718	3.992828879332718E-4	0.0
10	7.985657758665436E-4	0.0	0.005190677543132534	3.992828879332718E-4	0.0
11	7.985657758665436E-4	0.0	0.0073867334267655295	3.992828879332718E-4	0.0
12	7.985657758665436E-4	0.0	0.008185299202632073	3.992828879332718E-4	0.0
13	7.985657758665436E-4	0.0	0.009383147866431888	3.992828879332718E-4	0.0
14	7.985657758665436E-4	0.0	0.010181713642298432	3.992828879332718E-4	0.0
15	7.985657758665436E-4	0.0	0.011978486637998156	3.992828879332718E-4	0.0
16	7.985657758665436E-4	0.0	0.012377769525931427	5.989243318999078E-4	0.0
17	7.985657758665436E-4	0.0	0.013176335301797971	5.989243318999078E-4	0.0
18	7.985657758665436E-4	0.0	0.013375976745764608	5.989243318999078E-4	0.0
19	7.985657758665436E-4	0.0	0.013375976745764608	7.985657758665436E-4	0.0
20	7.985657758665436E-4	0.0	0.013974901077664515	9.982072198331795E-4	0.0
21	7.985657758665436E-4	0.0	0.014374183965597786	0.0011978486637998155	0.0
22	7.985657758665436E-4	0.0	0.014374183965597786	0.0011978486637998155	0.0
23	7.985657758665436E-4	0.0	0.014773466853531059	0.0011978486637998155	0.0
24	7.985657758665436E-4	0.0	0.014773466853531059	0.0011978486637998155	0.0
25	7.985657758665436E-4	0.0	0.014973108297497694	0.0017967729956997233	0.0
26	7.985657758665436E-4	0.0	0.014973108297497694	0.001996414439666359	0.0
27	7.985657758665436E-4	0.0	0.014973108297497694	0.0033939045474328106	0.0
28	7.985657758665436E-4	0.0	0.014973108297497694	0.01217812808196479	0.0
29	7.985657758665436E-4	0.0	0.014973108297497694	0.023757331832029674	0.0
30	7.985657758665436E-4	0.0	0.014973108297497694	0.04871251232785916	0.0
31	7.985657758665436E-4	0.0	0.014973108297497694	0.11479383028081566	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	215	0.0	30.976744	1
TTAACGG	110	3.6379788E-12	23.545454	35
TAACGGC	115	5.456968E-12	22.52174	36
GTATCAA	610	0.0	21.836065	1
ATTCGGA	65	6.892197E-5	19.923077	36
GTGTATA	75	9.248664E-6	19.733334	1
TTTAACG	135	5.638867E-11	19.185186	34
AACGGCC	145	7.2759576E-12	19.13793	37
AGCTTCG	60	9.226896E-4	18.5	21
TAGCCGG	50	0.007029476	18.5	30
CCGTCTA	60	9.226896E-4	18.5	26
CGCTCAC	80	1.613704E-5	18.5	10
ATTAGAG	165	3.6379788E-12	17.939396	3
GGCACCG	165	3.6379788E-12	17.939396	9
CCGTTAA	115	6.3835614E-8	17.695652	16
TAGGGGT	75	2.0643337E-4	17.266666	4
TTAGGCT	65	0.0015782518	17.076923	4
TTTTTCG	65	0.0015782518	17.076923	25
GTTCGCC	65	0.0015782518	17.076923	26
AGCGCCC	65	0.0015782518	17.076923	10
>>END_MODULE