FastQCFastQC Report
Fri 10 Feb 2017
ERR1632943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488483
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9710.1987786678349093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.1441196520656809No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6890.14104892084269052No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6670.136545181715638No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6330.12958485761019317No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5640.11545949398443754No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5300.10849916987899272No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5170.1058378694857344No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5010.10256242284787803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2600.027.0384621
CGAGTCG400.001929606423.12521
CGCCGTC400.001929606423.12526
AACGCCC652.6776052E-622.7692312
GTATCAA7500.022.6933331
CGAACCG753.7316568E-722.231
ATCCGTC502.6990206E-422.230
GCGAACC806.9432645E-720.812530
GTCCGGC450.003822705220.55555715
TCCGCCG555.1372766E-420.18181831
CGTCCTA555.1372766E-420.18181833
TTAGCCG656.8917514E-519.92307729
TCCGGCG500.007029232618.516
AACCGGG902.1470642E-618.533
AGCCGGT701.2173767E-418.531
TCCGCTA500.007029232618.510
TTTGTCG500.007029232618.536
GCCGGCA500.007029232618.515
ATAGAAC500.007029232618.53
TAGCCGG701.2173767E-418.530