##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632943.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488483
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68090598854003	33.0	33.0	33.0	27.0	33.0
2	31.20669296577363	33.0	33.0	33.0	27.0	33.0
3	31.63416741217197	33.0	33.0	33.0	27.0	33.0
4	31.780506179334797	33.0	33.0	33.0	27.0	33.0
5	31.890755666010897	33.0	33.0	33.0	33.0	33.0
6	34.75509076057918	37.0	37.0	37.0	27.0	37.0
7	34.93709709447412	37.0	37.0	37.0	27.0	37.0
8	35.11895193896205	37.0	37.0	37.0	33.0	37.0
9	35.19551755127609	37.0	37.0	37.0	33.0	37.0
10	35.237875217766025	37.0	37.0	37.0	33.0	37.0
11	35.26065799628646	37.0	37.0	37.0	33.0	37.0
12	35.19036281712977	37.0	37.0	37.0	33.0	37.0
13	35.24109129693357	37.0	37.0	37.0	33.0	37.0
14	35.209847630316716	37.0	37.0	37.0	33.0	37.0
15	35.22530569129325	37.0	37.0	37.0	33.0	37.0
16	35.224077398804056	37.0	37.0	37.0	33.0	37.0
17	35.186559204721554	37.0	37.0	37.0	33.0	37.0
18	35.20403780684282	37.0	37.0	37.0	33.0	37.0
19	35.22570693350639	37.0	37.0	37.0	33.0	37.0
20	35.21572500987752	37.0	37.0	37.0	33.0	37.0
21	35.20324760534143	37.0	37.0	37.0	33.0	37.0
22	35.21218957466278	37.0	37.0	37.0	33.0	37.0
23	35.20964291490185	37.0	37.0	37.0	33.0	37.0
24	35.20700003889593	37.0	37.0	37.0	33.0	37.0
25	35.19339874673223	37.0	37.0	37.0	33.0	37.0
26	35.15057432909641	37.0	37.0	37.0	33.0	37.0
27	35.099385239609155	37.0	37.0	37.0	33.0	37.0
28	35.07626058634589	37.0	37.0	37.0	33.0	37.0
29	35.12265933512528	37.0	37.0	37.0	33.0	37.0
30	35.084567937881154	37.0	37.0	37.0	33.0	37.0
31	35.07066776121175	37.0	37.0	37.0	27.0	37.0
32	35.12011881682679	37.0	37.0	37.0	33.0	37.0
33	35.09314551376404	37.0	37.0	37.0	33.0	37.0
34	35.04751649494455	37.0	37.0	37.0	27.0	37.0
35	35.04010784408055	37.0	37.0	37.0	27.0	37.0
36	35.02767547693574	37.0	37.0	37.0	27.0	37.0
37	35.04011193838885	37.0	37.0	37.0	27.0	37.0
38	34.90138244319659	37.0	37.0	37.0	27.0	37.0
39	34.98806509131331	37.0	37.0	37.0	27.0	37.0
40	35.026307977964436	37.0	37.0	37.0	27.0	37.0
41	35.05268965347822	37.0	37.0	37.0	27.0	37.0
42	34.95945201777749	37.0	37.0	37.0	27.0	37.0
43	34.75860777140658	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	22.0
17	24.0
18	21.0
19	24.0
20	33.0
21	86.0
22	265.0
23	645.0
24	1300.0
25	2525.0
26	3996.0
27	6238.0
28	8662.0
29	11638.0
30	15238.0
31	19643.0
32	24710.0
33	32365.0
34	45724.0
35	82677.0
36	232638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.44787638464389	20.556907814601534	13.479281776438484	25.515934024316095
2	16.151432086684693	22.586865868413025	37.44019750943226	23.821504535470016
3	17.59958893144695	26.509827363490647	30.05508891814045	25.83549478692196
4	12.829515049653725	17.852617184221355	38.71188966658	30.605978099544917
5	14.049618922255227	36.88910361261293	35.53163569663632	13.529641768495527
6	30.176485159155998	39.290620144406255	17.414935627237796	13.117959069199953
7	27.169010999359237	31.754431576943315	23.537359539635975	17.539197884061473
8	24.551724420297123	35.84444085055161	20.490375304770073	19.113459424381197
9	25.77367073163242	15.471162763084898	20.25167713103629	38.50348937424639
10	15.06275551042718	27.926458034363527	34.04949609300631	22.961290362202984
11	34.33220808093629	23.240317472665375	24.068596041213308	18.35887840518503
12	23.164777484579812	25.677863917475126	29.899095772012537	21.25826282593253
13	27.594000200621103	21.99605718110968	25.88278404775601	24.5271585705132
14	22.148570165184868	20.677894624787353	27.09838418123046	30.075151028797322
15	24.12857765776905	28.60918394294172	24.049762223045633	23.212476176243594
16	23.480653369718084	28.1688410855649	24.883363392380083	23.46714215233693
17	22.742859014540937	27.210772943991913	26.1521895337197	23.894178507747455
18	22.92894532665415	27.3632859280671	27.40934689641195	22.2984218488668
19	24.802500803508003	26.249634071195928	26.610137916774995	22.337727208521073
20	24.13123895816231	26.567352395067996	26.84044275849927	22.46096588827042
21	23.259765437077647	27.10063605079399	26.80257040674906	22.837028105379307
22	23.015130516312748	26.82242780199106	26.682607173637567	23.479834508058623
23	23.0624197771468	26.714747493771533	27.352231295664332	22.870601433417335
24	23.590790262916006	27.355711457717053	25.447559075750846	23.605939203616096
25	23.560901812345566	26.969004039035134	26.906770552915866	22.563323595703434
26	23.46243369779501	27.104935074506177	26.702873999709304	22.72975722798951
27	23.12014952413902	26.930517541040324	26.24390203957968	23.70543089524098
28	22.79403786825744	26.623444418741286	27.24434627202994	23.338171440971333
29	24.280271780184776	26.62835758869807	26.38290380627371	22.70846682484344
30	23.682093337946252	26.424665750906378	26.894897058853633	22.998343852293736
31	23.601640179903907	26.589052229043798	26.72743984949323	23.081867741559073
32	22.596282777496864	26.557116624324696	27.05170906664101	23.79489153153743
33	22.612045864441548	26.674009126213193	27.58192199114401	23.13202301820125
34	23.604301480297167	25.98841720182688	27.16655441438085	23.24072690349511
35	23.050341567669705	27.105753936165637	26.82242780199106	23.021476694173597
36	24.044644337673983	26.69693725267819	26.257003826131104	23.001414583516723
37	23.164368053750078	26.250862363685123	26.716385217090462	23.868384365474334
38	23.410845413248772	26.622625557081825	26.511669802224436	23.45485922744497
39	22.949621583555622	26.60358702349928	27.111076536952154	23.335714855992943
40	22.954534753512405	27.1933721337283	27.01506500737999	22.837028105379307
41	23.093127089376704	25.9292544469306	27.07688906266953	23.90072940102317
42	22.740811860392277	27.011789560742134	27.05068548956668	23.19671308929891
43	23.48085808513295	25.6635338384345	27.169010999359237	23.68659707707331
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	11.0
2	17.0
3	57.5
4	98.0
5	98.0
6	130.5
7	163.0
8	144.5
9	126.0
10	188.0
11	250.0
12	250.0
13	386.5
14	523.0
15	938.0
16	1353.0
17	1609.0
18	1865.0
19	1865.0
20	1816.5
21	1768.0
22	2215.0
23	2662.0
24	3752.0
25	4842.0
26	4842.0
27	6407.5
28	7973.0
29	10111.0
30	12249.0
31	15114.5
32	17980.0
33	17980.0
34	21177.0
35	24374.0
36	27440.0
37	30506.0
38	33575.5
39	36645.0
40	36645.0
41	38707.5
42	40770.0
43	42046.0
44	43322.0
45	42117.5
46	40913.0
47	40913.0
48	39335.0
49	37757.0
50	37476.5
51	37196.0
52	35370.0
53	33544.0
54	33544.0
55	31728.0
56	29912.0
57	26046.0
58	22180.0
59	20619.5
60	19059.0
61	19059.0
62	16733.0
63	14407.0
64	11838.5
65	9270.0
66	7800.5
67	6331.0
68	6331.0
69	5206.5
70	4082.0
71	3295.5
72	2509.0
73	2054.0
74	1599.0
75	1599.0
76	1262.5
77	926.0
78	727.0
79	528.0
80	431.5
81	335.0
82	335.0
83	257.0
84	179.0
85	164.5
86	150.0
87	113.0
88	76.0
89	76.0
90	54.5
91	33.0
92	19.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	488483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.113678059428565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.43417752221364	51.09442143379634
2	7.161406976469835	8.466742129191669
3	2.1999907596296007	3.9014863649538567
4	1.0479715928912803	2.477978214304067
5	0.6468785878981131	1.9119686294273412
6	0.41885738746061296	1.4856120453096
7	0.30820557155936534	1.2753415453297747
8	0.22092267968252904	1.0447641730223425
9	0.16749229983183753	0.8910977300723272
>10	1.1947073303230609	13.662913651485193
>50	0.12697949657058788	5.25559825296262
>100	0.06926154349248387	7.337696876776829
>500	0.003148251976931085	1.1943789533679856
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	971	0.1987786678349093	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	704	0.1441196520656809	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	689	0.14104892084269052	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	667	0.136545181715638	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	633	0.12958485761019317	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	564	0.11545949398443754	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	530	0.10849916987899272	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	517	0.1058378694857344	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	501	0.10256242284787803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.04715414866024E-4	0.0	0.0
2	0.0	0.0	2.04715414866024E-4	0.0	0.0
3	0.0	0.0	4.09430829732048E-4	0.0	0.0
4	0.0	0.0	4.09430829732048E-4	2.04715414866024E-4	0.0
5	0.0	0.0	4.09430829732048E-4	2.04715414866024E-4	0.0
6	0.0	0.0	0.001228292489196144	2.04715414866024E-4	0.0
7	0.0	0.0	0.00307073122299036	2.04715414866024E-4	0.0
8	0.0	0.0	0.00409430829732048	2.04715414866024E-4	0.0
9	0.0	0.0	0.0051178853716506	4.09430829732048E-4	0.0
10	2.04715414866024E-4	0.0	0.005732031616248672	4.09430829732048E-4	0.0
11	2.04715414866024E-4	0.0	0.008393332009506984	4.09430829732048E-4	0.0
12	2.04715414866024E-4	0.0	0.009416909083837103	4.09430829732048E-4	0.0
13	2.04715414866024E-4	0.0	0.010440486158167225	4.09430829732048E-4	0.0
14	2.04715414866024E-4	0.0	0.01125934781763132	4.09430829732048E-4	0.0
15	2.04715414866024E-4	0.0	0.011464063232497344	4.09430829732048E-4	0.0
16	2.04715414866024E-4	0.0	0.011668778647363367	4.09430829732048E-4	0.0
17	2.04715414866024E-4	0.0	0.011668778647363367	4.09430829732048E-4	0.0
18	2.04715414866024E-4	0.0	0.011873494062229392	4.09430829732048E-4	0.0
19	2.04715414866024E-4	0.0	0.012487640306827464	4.09430829732048E-4	0.0
20	2.04715414866024E-4	0.0	0.012897071136559511	4.09430829732048E-4	0.0
21	2.04715414866024E-4	0.0	0.013306501966291559	4.09430829732048E-4	0.0
22	2.04715414866024E-4	0.0	0.013511217381157584	6.14146244598072E-4	0.0
23	2.04715414866024E-4	0.0	0.013715932796023608	6.14146244598072E-4	0.0
24	2.04715414866024E-4	0.0	0.014125363625755656	0.00102357707433012	0.0
25	2.04715414866024E-4	0.0	0.014125363625755656	0.00102357707433012	0.0
26	2.04715414866024E-4	0.0	0.014125363625755656	0.001637723318928192	0.0
27	2.04715414866024E-4	0.0	0.014125363625755656	0.002661300393258312	0.0
28	2.04715414866024E-4	0.0	0.014125363625755656	0.010440486158167225	0.0
29	2.04715414866024E-4	0.0	0.014125363625755656	0.026408288517717097	0.0
30	2.04715414866024E-4	2.04715414866024E-4	0.014125363625755656	0.04810812249351564	0.0
31	2.04715414866024E-4	2.04715414866024E-4	0.014125363625755656	0.1117746165168491	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	260	0.0	27.038462	1
CGAGTCG	40	0.0019296064	23.125	21
CGCCGTC	40	0.0019296064	23.125	26
AACGCCC	65	2.6776052E-6	22.76923	12
GTATCAA	750	0.0	22.693333	1
CGAACCG	75	3.7316568E-7	22.2	31
ATCCGTC	50	2.6990206E-4	22.2	30
GCGAACC	80	6.9432645E-7	20.8125	30
GTCCGGC	45	0.0038227052	20.555557	15
TCCGCCG	55	5.1372766E-4	20.181818	31
CGTCCTA	55	5.1372766E-4	20.181818	33
TTAGCCG	65	6.8917514E-5	19.923077	29
TCCGGCG	50	0.0070292326	18.5	16
AACCGGG	90	2.1470642E-6	18.5	33
AGCCGGT	70	1.2173767E-4	18.5	31
TCCGCTA	50	0.0070292326	18.5	10
TTTGTCG	50	0.0070292326	18.5	36
GCCGGCA	50	0.0070292326	18.5	15
ATAGAAC	50	0.0070292326	18.5	3
TAGCCGG	70	1.2173767E-4	18.5	30
>>END_MODULE