FastQCFastQC Report
Fri 10 Feb 2017
ERR1632942.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632942.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461058
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11290.24487157797934317No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7960.17264639156028092No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7760.168308542526103No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7540.1635369085885073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.16245244632996284No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7300.1583314897474938No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5980.12970168612191957No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5760.12493005218432388No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5750.12471315973261497No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5740.1244962672809061No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5720.1240624823774883No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC5520.11972463334331039No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT5260.1140854295988791No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA5080.11018136546811898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6650.023.6466181
GGTATCA2600.022.0576921
CGAACCG855.163747E-821.76470631
AACGATT450.003822385820.55555522
GCTCCGA909.447467E-820.55555529
CTAGCAT450.003822385820.5555554
CTAATAC1550.020.2903233
ATGGGCG555.136675E-420.18181811
CCAATAC759.2460505E-619.7333323
AGGCGAA951.6706872E-719.47368428
TCGCCAT1355.638867E-1119.18518413
GTCGCCA1355.638867E-1119.18518412
GTCGCCC902.146533E-618.537
GCGAACC1002.865654E-718.530
TTCAACG609.2253793E-418.519
TATTCCG609.2253793E-418.55
TGCCTAG500.00702864818.510
CGCAAGG500.00702864818.510
GGCGAAC1002.865654E-718.529
CTCTAAT1103.8367943E-818.51
Produced by FastQC (version 0.11.5)