FastQCFastQC Report
Fri 10 Feb 2017
ERR1632941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532976
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51880.9734021794602383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36500.6848338386719102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18630.34954669628651197No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12050.22608897961634294No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10840.20338626880009605No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10580.19850800036024135No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10410.1953183633034133No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10400.19513073759418811No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10340.194004983338837No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10140.1902524691543334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.18968959202665786No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9980.1872504578067305No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9780.18349794362222688No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9550.17918255231004773No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9340.17524241241631894No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9240.17336615532406713No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9200.1726156524871664No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9180.17224040106871605No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8950.16792500975653687No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8930.16754975833808652No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8890.1667992555011858No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8550.16041998138752966No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7910.14841193599711808No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7840.1470985560325418No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7640.14334604184803817No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7520.14109453333733601No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7360.1380925219897331No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7330.13752964486205757No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7290.13677914202515684No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6970.13077511932995106No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6960.1305874936207259No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6910.12964936507459998No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6850.1285236108192489No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6780.12721023085467265No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6770.12702260514544744No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6740.12645972801777192No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6620.12420821950706973No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6600.12383296808861938No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6580.12345771667016901No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6310.11839182252108914No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6190.11614031401038696No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6160.11557743688271141No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5910.11088679415208189No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5690.10675902854912793No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5600.10507039716610128No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5510.10338176578307465No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5490.10300651436462431No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5330.10000450301702139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCC200.001841050337.024
TCGAATC200.001841050337.023
ATATCTC250.00549373229.5999988
GCGATAT250.00549373229.59999817
CCTTGGG250.00549373229.5999982
CATCGCG405.934243E-527.75000222
GCCGCTC2100.027.30952327
GGTATCA8750.027.2742861
CCGCTCT2150.026.67441728
CGCTCTC2200.026.06818229
AGCTTCG2500.025.921
GGCAGCT2450.025.67347118
GCTCTCT2250.025.4888930
GCAGCTT2500.025.1619
TTCGCCG2450.024.9183724
GCTTCGC2550.024.66666622
GGTATGT451.321912E-424.6666661
GCCGGCA2350.024.40425715
GTATCAA25000.023.681
CTCTCTT2600.023.4807731