FastQCFastQC Report
Fri 10 Feb 2017
ERR1632940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538713
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48580.9017788692680517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37400.6942472151219666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18190.3376566000820474No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11750.21811242721077828No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11740.2179267996131521No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11140.2067891437555804No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10750.19954966744815886No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10560.19602274309326118No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9980.1852563424309419No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9870.18321443885705374No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9830.18247192846654897No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9790.1817294180760442No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9680.17968751450215606No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9610.17838812131877269No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9610.17838812131877269No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9490.17616059014725838No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9410.1746755693662488No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9390.17430431417099643No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9340.17337617618286547No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG8030.14905896089383402No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8000.14850207810095542No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7810.14497515374605774No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT7800.14478952614843155No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7650.1420051121840386No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7620.14144822939116006No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7480.13884944302439334No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7450.13829256023151473No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7390.13717879464575758No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7390.13717879464575758No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7390.13717879464575758No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7230.13420875308373847No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT6930.12863992515495262No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6840.1269692767763169No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6760.12548425599530735No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6620.12288546962854062No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6570.12195733164040963No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6510.12084356605465248No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6460.1199154280665215No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6420.11917291767601673No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6320.11731664169975478No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6290.11675975890687622No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA6210.11527473812586665No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6190.11490348293061425No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6130.1137897173448571No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG6010.11156218617334276No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5920.109891537794707No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC5910.10970591019708081No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5820.10803526181844508No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5810.10784963422081889No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5510.10228080629203304No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC5490.10190955109678065No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT5460.10135266830390208No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA5410.10042453031577112No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA5410.10042453031577112No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG5390.10005327512051872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGTG251.232755E-437.023
CCACGTT352.3859697E-531.71428737
AACCACG303.5969625E-430.83333235
TCTGTGC303.5969625E-430.83333224
CGGGGAC250.00549378329.616
CGGTGTA250.00549378329.634
GAATTAT250.00549378329.617
GGTATCA8200.027.7499981
TCTCTGT358.863252E-426.42857222
GTGCTAG358.863252E-426.42857227
TAACAAG358.863252E-426.42857231
GTAACCA358.863252E-426.42857233
GTCGGTG358.863252E-426.42857232
CGTTATT451.3219376E-424.66666610
ATTGGCG551.9002273E-523.54545437
GTATCAA23550.023.4883231
GCCGCTC2450.023.40816327
GTCCTCA400.00192986323.1256
TGTGCTA400.00192986323.12526
TAACCAC400.00192986323.12534
Produced by FastQC (version 0.11.5)