##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632940.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538713
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69997197023276	33.0	33.0	33.0	27.0	33.0
2	31.20385994026504	33.0	33.0	33.0	27.0	33.0
3	31.646073140986015	33.0	33.0	33.0	27.0	33.0
4	31.779348187253696	33.0	33.0	33.0	27.0	33.0
5	31.889913553227785	33.0	33.0	33.0	33.0	33.0
6	34.68722677937974	37.0	37.0	37.0	27.0	37.0
7	34.88449137110113	37.0	37.0	37.0	27.0	37.0
8	35.07115105817012	37.0	37.0	37.0	33.0	37.0
9	35.143685041942554	37.0	37.0	37.0	33.0	37.0
10	35.15417485748441	37.0	37.0	37.0	33.0	37.0
11	35.206042920813125	37.0	37.0	37.0	33.0	37.0
12	35.1075303547529	37.0	37.0	37.0	33.0	37.0
13	35.161497866210766	37.0	37.0	37.0	33.0	37.0
14	35.11812040919747	37.0	37.0	37.0	33.0	37.0
15	35.15880812232116	37.0	37.0	37.0	33.0	37.0
16	35.1631759396933	37.0	37.0	37.0	33.0	37.0
17	35.138532019832454	37.0	37.0	37.0	33.0	37.0
18	35.15303139148303	37.0	37.0	37.0	33.0	37.0
19	35.15710406097495	37.0	37.0	37.0	33.0	37.0
20	35.14019709938316	37.0	37.0	37.0	33.0	37.0
21	35.14086721501059	37.0	37.0	37.0	33.0	37.0
22	35.1352909619779	37.0	37.0	37.0	33.0	37.0
23	35.14757022756087	37.0	37.0	37.0	33.0	37.0
24	35.152584028972754	37.0	37.0	37.0	33.0	37.0
25	35.14066116837723	37.0	37.0	37.0	33.0	37.0
26	35.07594767529278	37.0	37.0	37.0	33.0	37.0
27	35.01315914039572	37.0	37.0	37.0	27.0	37.0
28	35.01601594912319	37.0	37.0	37.0	27.0	37.0
29	35.02979508569498	37.0	37.0	37.0	27.0	37.0
30	34.982686513969405	37.0	37.0	37.0	27.0	37.0
31	34.956169611648505	37.0	37.0	37.0	27.0	37.0
32	34.98502356542352	37.0	37.0	37.0	27.0	37.0
33	34.95190574573103	37.0	37.0	37.0	27.0	37.0
34	34.88715327085109	37.0	37.0	37.0	27.0	37.0
35	34.86903601732277	37.0	37.0	37.0	27.0	37.0
36	34.82571239231279	37.0	37.0	37.0	27.0	37.0
37	34.82197756504855	37.0	37.0	37.0	27.0	37.0
38	34.670015388527844	37.0	37.0	37.0	27.0	37.0
39	34.7389036462829	37.0	37.0	37.0	27.0	37.0
40	34.74100123813608	37.0	37.0	37.0	27.0	37.0
41	34.74192566357225	37.0	37.0	37.0	27.0	37.0
42	34.61371453816782	37.0	37.0	37.0	27.0	37.0
43	34.371635731827524	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	29.0
17	29.0
18	28.0
19	28.0
20	45.0
21	113.0
22	323.0
23	802.0
24	1628.0
25	3003.0
26	4843.0
27	7175.0
28	10272.0
29	13861.0
30	18285.0
31	22950.0
32	28659.0
33	37545.0
34	52181.0
35	92638.0
36	244269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99620205935257	20.476951549340743	13.684280869405416	23.84256552190127
2	16.77256721111241	23.204749096457668	37.88752081349438	22.13516287893554
3	17.081080278367146	28.832235346093377	30.47169828832792	23.61498608721156
4	11.425007378697005	18.4994607518289	41.30882306534277	28.76670880413133
5	13.036997436482878	37.49584658250311	35.652564538075005	13.814591442939006
6	26.58131509727814	42.9148730400046	16.776836645857813	13.726975216859442
7	26.063228472303436	32.82545622622807	23.551872704018653	17.55944259744985
8	24.279904141908588	34.639409110231234	20.22394113377624	20.856745614083938
9	26.80295444884382	14.204223770356386	20.349425389771547	38.64339639102825
10	16.623508250218578	26.6405303009209	33.16905290943415	23.56690853942637
11	33.878707215159096	24.433046909950196	23.4934000107664	18.19484586412431
12	23.75884747537186	27.48829153927973	27.906696144329167	20.846164841019245
13	28.184023775182705	22.945056087378624	25.51581268690379	23.355107450534888
14	22.531106544672213	21.218162546662136	28.613009153296836	27.637721755368815
15	25.25816158139863	27.57980594490944	26.254981780651292	20.907050693040635
16	22.130336561397257	28.295029078563168	25.729655679369163	23.844978680670412
17	22.144444258816847	26.49258510561282	25.818385671034484	25.54458496453585
18	23.162982886991777	26.307514390779506	28.32955581172164	22.19994691050708
19	26.253682387467908	24.648189295598954	28.620619884799513	20.47750843213362
20	24.919948098523705	24.839942603946813	28.83650478083878	21.403604516690706
21	22.420472496486997	27.27314915363097	28.54321317658939	21.763165173292645
22	22.496208556318482	26.65036856359509	28.42162710014423	22.431795779942195
23	22.179156619572947	26.72499085784082	29.729744780616024	21.366107741970215
24	22.658632704241406	27.75800843863059	26.46641161434753	23.116947242780476
25	22.94431357698812	26.75450564586338	29.32322034181466	20.97796043533384
26	22.446831615349918	27.43650143954202	28.557506501606607	21.559160443501458
27	22.5530106011921	26.815205870287144	27.91263622745321	22.719147301067544
28	21.59758535621008	26.65853617789064	29.74737940239051	21.99649906350877
29	24.514908680503346	26.38380733340387	27.433717025577625	21.667566960515153
30	23.643387109648366	25.97561224622387	28.786199701882076	21.594800942245683
31	22.783188822248583	26.388448023344523	28.743876609623303	22.084486544783584
32	20.958098282387837	26.02387542160668	29.223352694291766	23.794673601713715
33	20.912433893371794	26.247371049148622	30.079838429739027	22.76035662774056
34	22.607956370089454	25.778846992740107	29.514788022564893	22.09840861460555
35	22.28236556385311	27.205580708095034	28.375591455932934	22.13646227211892
36	23.546489503687493	26.840265595966684	27.51892009288805	22.094324807457774
37	22.374251224678076	26.013665903737245	28.282035146729335	23.330047724855348
38	22.44126278742113	26.404040741545128	28.054826967234874	23.09986950379887
39	21.61707625396083	26.51912985207337	28.719188139139025	23.14460575482678
40	21.5537772431703	27.621943409570587	29.549500383320986	21.274778963938125
41	22.217395904683944	25.64835079160889	28.620991139994768	23.513262163712405
42	21.05815155750836	28.00730630224257	28.442974273871247	22.491567866377828
43	22.691674416618866	25.594333160699666	28.54228503860126	23.171707384080207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	57.5
2	60.0
3	282.5
4	505.0
5	505.0
6	633.0
7	761.0
8	757.5
9	754.0
10	968.0
11	1182.0
12	1182.0
13	1915.0
14	2648.0
15	4472.0
16	6296.0
17	6632.0
18	6968.0
19	6968.0
20	5264.0
21	3560.0
22	2620.0
23	1680.0
24	1988.5
25	2297.0
26	2297.0
27	3232.0
28	4167.0
29	6297.0
30	8427.0
31	12299.5
32	16172.0
33	16172.0
34	21335.0
35	26498.0
36	29987.0
37	33476.0
38	37327.5
39	41179.0
40	41179.0
41	44962.5
42	48746.0
43	50721.0
44	52696.0
45	51413.5
46	50131.0
47	50131.0
48	48709.5
49	47288.0
50	47349.0
51	47410.0
52	43451.5
53	39493.0
54	39493.0
55	36874.5
56	34256.0
57	27283.5
58	20311.0
59	18775.0
60	17239.0
61	17239.0
62	14011.5
63	10784.0
64	7878.5
65	4973.0
66	4363.5
67	3754.0
68	3754.0
69	2946.0
70	2138.0
71	1671.5
72	1205.0
73	1132.0
74	1059.0
75	1059.0
76	640.0
77	221.0
78	163.5
79	106.0
80	95.5
81	85.0
82	85.0
83	75.0
84	65.0
85	52.5
86	40.0
87	27.5
88	15.0
89	15.0
90	12.5
91	10.0
92	6.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	538713.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.813798419095335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51807165394912	18.45999732204133
2	7.9301070033784935	3.7769193924062328
3	3.194350773836919	2.282088762241007
4	1.818111641880053	1.731845765725681
5	1.3220494068382054	1.5741509037264794
6	0.9238523565622075	1.3200260272907156
7	0.7556256867360039	1.2596022448955175
8	0.5728849336291669	1.0914053061425435
9	0.5137952586208727	1.1011875045735977
>10	4.209815717429402	21.195012927587502
>50	0.6373795648431066	10.62971260397997
>100	0.5565411810093468	25.670054399051683
>500	0.041196484069127626	6.944316129469448
>1k	0.006218337217981529	2.9636807108683048
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4858	0.9017788692680517	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3740	0.6942472151219666	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1819	0.3376566000820474	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1175	0.21811242721077828	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1174	0.2179267996131521	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1114	0.2067891437555804	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1075	0.19954966744815886	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1056	0.19602274309326118	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	998	0.1852563424309419	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	987	0.18321443885705374	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	983	0.18247192846654897	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	979	0.1817294180760442	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	968	0.17968751450215606	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	961	0.17838812131877269	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	961	0.17838812131877269	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	949	0.17616059014725838	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	941	0.1746755693662488	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	939	0.17430431417099643	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	934	0.17337617618286547	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	803	0.14905896089383402	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	800	0.14850207810095542	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	781	0.14497515374605774	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	780	0.14478952614843155	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	765	0.1420051121840386	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	762	0.14144822939116006	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	748	0.13884944302439334	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	745	0.13829256023151473	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	739	0.13717879464575758	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	739	0.13717879464575758	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	739	0.13717879464575758	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	723	0.13420875308373847	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	693	0.12863992515495262	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	684	0.1269692767763169	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	676	0.12548425599530735	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	662	0.12288546962854062	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	657	0.12195733164040963	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	651	0.12084356605465248	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	646	0.1199154280665215	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	642	0.11917291767601673	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	632	0.11731664169975478	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	629	0.11675975890687622	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	621	0.11527473812586665	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	619	0.11490348293061425	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	613	0.1137897173448571	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	601	0.11156218617334276	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	592	0.109891537794707	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	591	0.10970591019708081	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	582	0.10803526181844508	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	581	0.10784963422081889	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	551	0.10228080629203304	No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC	549	0.10190955109678065	No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT	546	0.10135266830390208	No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA	541	0.10042453031577112	No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA	541	0.10042453031577112	No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG	539	0.10005327512051872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.568827928785829E-4	0.0	0.0
2	1.8562759762619428E-4	0.0	0.0011137655857571657	0.0	0.0
3	1.8562759762619428E-4	0.0	0.002041903573888137	0.0	0.0
4	1.8562759762619428E-4	0.0	0.00259878636676672	0.0	0.0
5	3.7125519525238855E-4	0.0	0.0035269243548976914	0.0	0.0
6	5.568827928785829E-4	0.0	0.007239476307421577	0.0	0.0
7	5.568827928785829E-4	0.0	0.018562759762619428	0.0	0.0
8	7.425103905047771E-4	0.0	0.020604663336507564	0.0	0.0
9	7.425103905047771E-4	0.0	0.027472884448676755	3.7125519525238855E-4	0.0
10	0.0022275311715143314	0.0	0.030628553608322055	5.568827928785829E-4	0.0
11	0.0022275311715143314	0.0	0.04807754778518432	5.568827928785829E-4	0.0
12	0.0022275311715143314	0.0	0.0519757273353344	7.425103905047771E-4	0.0
13	0.0022275311715143314	0.0	0.055688279287858286	7.425103905047771E-4	0.0
14	0.0022275311715143314	0.0	0.06070022442376553	7.425103905047771E-4	0.0
15	0.0022275311715143314	0.0	0.06756844553593472	7.425103905047771E-4	0.0
16	0.0024131587691405255	0.0	0.07165225268371099	7.425103905047771E-4	0.0
17	0.0024131587691405255	0.0	0.0744366666481039	7.425103905047771E-4	0.0
18	0.00259878636676672	0.0	0.07629294262436585	9.281379881309714E-4	0.0
19	0.00259878636676672	0.0	0.07740670821012302	9.281379881309714E-4	0.0
20	0.00259878636676672	0.0	0.07963423938163734	0.00129939318338336	0.0
21	0.0027844139643929144	0.0	0.08204739815077787	0.0018562759762619427	0.0
22	0.0027844139643929144	0.0	0.08353241893178742	0.0027844139643929144	0.0
23	0.0027844139643929144	0.0	0.0842749293222922	0.004269434745402469	0.0
24	0.0027844139643929144	0.0	0.08520306731042318	0.006125710721664411	0.0
25	0.0027844139643929144	0.0	0.08538869490804937	0.0064969659169168	0.0
26	0.0027844139643929144	0.0	0.08538869490804937	0.007053848709795383	0.0
27	0.0027844139643929144	0.0	0.08557432250567557	0.009467007478935908	0.0
28	0.0027844139643929144	0.0	0.08557432250567557	0.028772277632060115	0.0
29	0.0027844139643929144	0.0	0.08575995010330176	0.07629294262436585	0.0
30	0.0027844139643929144	0.0	0.08575995010330176	0.1388494430243933	0.0
31	0.0027844139643929144	0.0	0.08575995010330176	0.2899503074921155	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTGTG	25	1.232755E-4	37.0	23
CCACGTT	35	2.3859697E-5	31.714287	37
AACCACG	30	3.5969625E-4	30.833332	35
TCTGTGC	30	3.5969625E-4	30.833332	24
CGGGGAC	25	0.005493783	29.6	16
CGGTGTA	25	0.005493783	29.6	34
GAATTAT	25	0.005493783	29.6	17
GGTATCA	820	0.0	27.749998	1
TCTCTGT	35	8.863252E-4	26.428572	22
GTGCTAG	35	8.863252E-4	26.428572	27
TAACAAG	35	8.863252E-4	26.428572	31
GTAACCA	35	8.863252E-4	26.428572	33
GTCGGTG	35	8.863252E-4	26.428572	32
CGTTATT	45	1.3219376E-4	24.666666	10
ATTGGCG	55	1.9002273E-5	23.545454	37
GTATCAA	2355	0.0	23.488323	1
GCCGCTC	245	0.0	23.408163	27
GTCCTCA	40	0.001929863	23.125	6
TGTGCTA	40	0.001929863	23.125	26
TAACCAC	40	0.001929863	23.125	34
>>END_MODULE