FastQCFastQC Report
Fri 10 Feb 2017
ERR1632939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466954
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16370.3505698634126702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.252915704758927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5940.12720739087790234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3100.024.4677431
TTAACGG701.9178333E-723.78571535
GTATCAA8550.022.5029241
TGGACGT502.6987738E-422.226
CTTGCGG502.6987738E-422.234
AGACCGG502.6987738E-422.221
GTACTAT603.7201895E-521.5833341
TTTAACG806.9419184E-720.812534
GCGGTAC450.003822457320.55555737
TGCGGTA450.003822457320.55555736
GACCGGA555.136812E-420.18181822
CGAACCG1202.382876E-1020.04166831
ACAATCC656.8909205E-519.9230778
TAACGGC851.2420714E-619.58823636
ATTAGGC500.0070287818.519
GTTTAAC902.1466512E-618.533
CATTAGG609.2256226E-418.518
CGGAGTA609.2256226E-418.525
CCTTGCG500.0070287818.533
TGCCGAT500.0070287818.512
Produced by FastQC (version 0.11.5)