FastQCFastQC Report
Fri 10 Feb 2017
ERR1632938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487986
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14790.30308246548056705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.23299848766153128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2200.027.751
CCGTTTA358.862053E-426.4285727
GCCGTTT358.862053E-426.4285726
ATTAGAT551.8997513E-523.5454543
CGTCAAT551.8997513E-523.54545430
GTATCAA6950.023.1582741
TAAACCG400.001929603423.1255
ACCTAAG450.003822700420.5555551
CGTCAGA909.450196E-820.55555512
GACAATT1001.2852979E-820.3534
GCGTCAG1001.2852979E-820.3511
GGACCGT555.1372667E-420.1818186
ACGTCAA656.891731E-519.92307729
CTAATAC1254.110916E-1019.2400023
CTGCGTC1355.638867E-1119.1851869
AACGTCA801.6135655E-518.528
CGGGTAA701.2173738E-418.523
GACCGTT500.00702922218.57
GCATTAC500.00702922218.51
GCGGGTA701.2173738E-418.522
Produced by FastQC (version 0.11.5)