##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632938.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487986
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.735789551339586	33.0	33.0	33.0	27.0	33.0
2	31.23815642252032	33.0	33.0	33.0	27.0	33.0
3	31.680271565167853	33.0	33.0	33.0	27.0	33.0
4	31.809951515002478	33.0	33.0	33.0	27.0	33.0
5	31.91520453455632	33.0	33.0	33.0	33.0	33.0
6	34.77814732389864	37.0	37.0	37.0	27.0	37.0
7	34.9650420298943	37.0	37.0	37.0	27.0	37.0
8	35.13132753808511	37.0	37.0	37.0	33.0	37.0
9	35.21918251753124	37.0	37.0	37.0	33.0	37.0
10	35.26191325160968	37.0	37.0	37.0	33.0	37.0
11	35.29542445889841	37.0	37.0	37.0	33.0	37.0
12	35.22442037271561	37.0	37.0	37.0	33.0	37.0
13	35.26906919460804	37.0	37.0	37.0	33.0	37.0
14	35.23578545286135	37.0	37.0	37.0	33.0	37.0
15	35.26712651592464	37.0	37.0	37.0	33.0	37.0
16	35.252617902972624	37.0	37.0	37.0	33.0	37.0
17	35.217512387650466	37.0	37.0	37.0	33.0	37.0
18	35.23944539392524	37.0	37.0	37.0	33.0	37.0
19	35.24644354551155	37.0	37.0	37.0	33.0	37.0
20	35.258878328476634	37.0	37.0	37.0	33.0	37.0
21	35.236457603291896	37.0	37.0	37.0	33.0	37.0
22	35.22146127142992	37.0	37.0	37.0	33.0	37.0
23	35.21533199722943	37.0	37.0	37.0	33.0	37.0
24	35.233746459939425	37.0	37.0	37.0	33.0	37.0
25	35.230221768657294	37.0	37.0	37.0	33.0	37.0
26	35.16617484927846	37.0	37.0	37.0	33.0	37.0
27	35.117618128388926	37.0	37.0	37.0	33.0	37.0
28	35.09234486235261	37.0	37.0	37.0	33.0	37.0
29	35.142450808014985	37.0	37.0	37.0	33.0	37.0
30	35.10512801596767	37.0	37.0	37.0	33.0	37.0
31	35.10077338284295	37.0	37.0	37.0	33.0	37.0
32	35.13098941363072	37.0	37.0	37.0	33.0	37.0
33	35.08960707889161	37.0	37.0	37.0	33.0	37.0
34	35.03621210444562	37.0	37.0	37.0	27.0	37.0
35	35.04073887365621	37.0	37.0	37.0	27.0	37.0
36	35.020500588131625	37.0	37.0	37.0	27.0	37.0
37	35.029656588508686	37.0	37.0	37.0	27.0	37.0
38	34.89013414319263	37.0	37.0	37.0	27.0	37.0
39	34.97259552528146	37.0	37.0	37.0	27.0	37.0
40	34.982997463041976	37.0	37.0	37.0	27.0	37.0
41	35.00764366190833	37.0	37.0	37.0	27.0	37.0
42	34.920561245609505	37.0	37.0	37.0	27.0	37.0
43	34.69991147287012	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	11.0
16	19.0
17	19.0
18	21.0
19	22.0
20	31.0
21	94.0
22	282.0
23	654.0
24	1365.0
25	2502.0
26	4013.0
27	5950.0
28	8601.0
29	11456.0
30	15159.0
31	19234.0
32	24368.0
33	32420.0
34	45388.0
35	82943.0
36	233428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.855270438086336	20.047501362744015	13.438910132667742	25.658318066501906
2	16.001073801297576	22.945945170558172	37.11745828773776	23.935522740406487
3	18.05502616878353	26.11242945494338	30.01418073469321	25.818363641579882
4	13.189927579889588	17.72284450783424	38.24331025890087	30.843917653375303
5	14.606361657916416	36.44592262892788	34.74812802006615	14.199587693089555
6	30.486325427368815	38.849475189862005	17.2859467279799	13.378252654789277
7	26.992372732004604	31.861774723045333	23.3418581680622	17.80399437688786
8	25.142319656711464	35.118220604689476	20.804080444930797	18.935379293668262
9	26.45383269192149	15.038136339976965	19.84196267925719	38.66606828884435
10	15.03096400306566	28.012688888615656	33.720229678720294	23.23611742959839
11	34.37106802244327	23.25804428815581	24.012164283401574	18.358723405999353
12	22.929141409794543	25.08596558097978	30.44206186243048	21.542831146795194
13	28.61885381957679	21.615579135467	25.956482358100434	23.80908468685577
14	22.392650608828944	20.382551958457825	27.24381437172378	29.980983060989452
15	23.955605283758143	28.43421737508863	23.691663285422123	23.91851405573111
16	23.345341874561974	27.867397835183795	25.005430483661417	23.78182980659281
17	22.800039345391056	26.861016504571854	26.510391691564923	23.828552458472167
18	23.68654018762833	26.555474952150266	27.130901296348664	22.627083563872734
19	24.696405224740055	26.137840020000574	26.94687962359576	22.218875131663612
20	24.44209465025636	26.074313607357585	26.637649440762644	22.845942301623406
21	23.723631415655365	26.42104486604124	26.707323570758177	23.14800014754522
22	23.43202468923289	26.245425073670148	26.816548015721764	23.5060022213752
23	23.520551819109563	26.031894357625013	27.100777481321188	23.346776341944235
24	23.40210579811716	26.55588479997377	26.381289627161436	23.660719774747633
25	23.793920317386156	26.216530802113176	26.954461808330564	23.035087072170104
26	23.499034808375647	26.486825441713492	26.688060723053532	23.32607902685733
27	23.61174295983901	26.479448180890437	26.483136811301968	23.42567204796859
28	23.385506961265282	26.249113704081672	26.875566102306216	23.48981323234683
29	23.790026763062873	26.49153869168378	26.399732779219075	23.318701766034273
30	23.78141995876931	26.518383724123236	26.614083190911213	23.086113126196242
31	23.845356219235796	26.157717639440474	26.7237174836983	23.273208657625425
32	23.007422344083643	26.323706007959245	27.01081588406225	23.65805576389487
33	23.308455570446693	26.258130356198745	26.934994036714166	23.498420036640397
34	23.499239732287403	26.095625694179752	26.867574069747903	23.537560503784942
35	23.39329406991184	26.512440930682434	26.849540765513762	23.244724233891954
36	23.750681372006575	26.225752378141998	26.673101277495665	23.350464972355763
37	23.490018156258582	26.10464234629682	26.821261265692048	23.58407823175255
38	23.555593808019083	26.419200550835477	26.49010422430152	23.535101416843926
39	23.197591734189096	26.14952068297041	27.052620362059564	23.600267220780925
40	23.34862065715	26.156283172058213	27.28438930625059	23.210706864541194
41	23.470345460730428	25.759550478907183	27.036841220854697	23.73326283950769
42	23.112138462988693	26.335591594840835	27.016143905767787	23.53612603640268
43	23.337144918091912	25.87041431516478	27.05938285114737	23.73305791559594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	13.5
2	24.0
3	94.0
4	164.0
5	164.0
6	219.0
7	274.0
8	268.5
9	263.0
10	333.5
11	404.0
12	404.0
13	650.0
14	896.0
15	1501.0
16	2106.0
17	2396.0
18	2686.0
19	2686.0
20	2505.5
21	2325.0
22	2776.0
23	3227.0
24	4335.0
25	5443.0
26	5443.0
27	7032.5
28	8622.0
29	10978.0
30	13334.0
31	15628.0
32	17922.0
33	17922.0
34	20551.0
35	23180.0
36	25507.0
37	27834.0
38	30089.5
39	32345.0
40	32345.0
41	34327.5
42	36310.0
43	37091.5
44	37873.0
45	38315.5
46	38758.0
47	38758.0
48	38252.0
49	37746.0
50	37376.0
51	37006.0
52	35792.0
53	34578.0
54	34578.0
55	32425.0
56	30272.0
57	27618.0
58	24964.0
59	23047.5
60	21131.0
61	21131.0
62	18551.0
63	15971.0
64	13487.0
65	11003.0
66	9491.5
67	7980.0
68	7980.0
69	6644.5
70	5309.0
71	4302.5
72	3296.0
73	2683.0
74	2070.0
75	2070.0
76	1607.5
77	1145.0
78	889.0
79	633.0
80	525.5
81	418.0
82	418.0
83	318.5
84	219.0
85	186.0
86	153.0
87	112.5
88	72.0
89	72.0
90	48.0
91	24.0
92	13.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	487986.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.43799723013748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.57558484862679	64.16237969922635
2	6.654821351295023	9.641238612243374
3	1.8778664284004791	4.0808664941712625
4	0.8212156196254677	2.3794885911910106
5	0.468338643884551	1.6962756694237722
6	0.34501452077674344	1.499529654022977
7	0.22864554056904596	1.1593837524096688
8	0.14832116487860936	0.8595270504517974
9	0.12735675967145743	0.8302921743888287
>10	0.6959866396367042	9.353333411006357
>50	0.0400781811527687	2.0097851400029305
>100	0.01620181668635502	1.789266658429306
>500	0.0	0.0
>1k	5.684847960124448E-4	0.5386330930323358
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1479	0.30308246548056705	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1137	0.23299848766153128	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.098478235031333E-4	0.0	0.0
2	0.0	0.0	8.196956470062665E-4	0.0	0.0
3	0.0	0.0	0.0012295434705094	0.0	0.0
4	0.0	0.0	0.001639391294012533	0.0	0.0
5	0.0	2.0492391175156664E-4	0.0024590869410188	0.0	0.0
6	0.0	2.0492391175156664E-4	0.0043034021467829	0.0	2.0492391175156664E-4
7	0.0	2.0492391175156664E-4	0.007377260823056399	0.0	2.0492391175156664E-4
8	0.0	2.0492391175156664E-4	0.009631423852323632	0.0	2.0492391175156664E-4
9	0.0	2.0492391175156664E-4	0.010451119499329899	0.0	2.0492391175156664E-4
10	0.0	2.0492391175156664E-4	0.013115130352100265	0.0	2.0492391175156664E-4
11	4.098478235031333E-4	2.0492391175156664E-4	0.02192685855741763	0.0	2.0492391175156664E-4
12	4.098478235031333E-4	2.0492391175156664E-4	0.023566249851430163	0.0	2.0492391175156664E-4
13	4.098478235031333E-4	2.0492391175156664E-4	0.024590869410187996	0.0	2.0492391175156664E-4
14	4.098478235031333E-4	2.0492391175156664E-4	0.027254880262958362	0.0	2.0492391175156664E-4
15	4.098478235031333E-4	2.0492391175156664E-4	0.02930411938047403	0.0	2.0492391175156664E-4
16	4.098478235031333E-4	2.0492391175156664E-4	0.030738586762734995	0.0	2.0492391175156664E-4
17	4.098478235031333E-4	2.0492391175156664E-4	0.03114843458623813	0.0	2.0492391175156664E-4
18	4.098478235031333E-4	2.0492391175156664E-4	0.03155828240974126	2.0492391175156664E-4	2.0492391175156664E-4
19	4.098478235031333E-4	2.0492391175156664E-4	0.03176320632149283	2.0492391175156664E-4	2.0492391175156664E-4
20	4.098478235031333E-4	2.0492391175156664E-4	0.032173054144995966	2.0492391175156664E-4	2.0492391175156664E-4
21	4.098478235031333E-4	2.0492391175156664E-4	0.032787825880250664	2.0492391175156664E-4	2.0492391175156664E-4
22	4.098478235031333E-4	2.0492391175156664E-4	0.03340259761550536	6.147717352547E-4	2.0492391175156664E-4
23	4.098478235031333E-4	2.0492391175156664E-4	0.034017369350760066	0.0012295434705094	2.0492391175156664E-4
24	4.098478235031333E-4	2.0492391175156664E-4	0.034017369350760066	0.0012295434705094	2.0492391175156664E-4
25	4.098478235031333E-4	2.0492391175156664E-4	0.03422229326251163	0.0012295434705094	2.0492391175156664E-4
26	4.098478235031333E-4	2.0492391175156664E-4	0.03422229326251163	0.002254163029267233	2.0492391175156664E-4
27	4.098478235031333E-4	2.0492391175156664E-4	0.03422229326251163	0.004098478235031333	2.0492391175156664E-4
28	4.098478235031333E-4	2.0492391175156664E-4	0.03422229326251163	0.013320054263851832	2.0492391175156664E-4
29	4.098478235031333E-4	2.0492391175156664E-4	0.03422229326251163	0.030123815027480298	2.0492391175156664E-4
30	4.098478235031333E-4	2.0492391175156664E-4	0.03422229326251163	0.05696884746693553	2.0492391175156664E-4
31	4.098478235031333E-4	2.0492391175156664E-4	0.03422229326251163	0.13381531437377303	2.0492391175156664E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	220	0.0	27.75	1
CCGTTTA	35	8.862053E-4	26.42857	27
GCCGTTT	35	8.862053E-4	26.42857	26
ATTAGAT	55	1.8997513E-5	23.545454	3
CGTCAAT	55	1.8997513E-5	23.545454	30
GTATCAA	695	0.0	23.158274	1
TAAACCG	40	0.0019296034	23.125	5
ACCTAAG	45	0.0038227004	20.555555	1
CGTCAGA	90	9.450196E-8	20.555555	12
GACAATT	100	1.2852979E-8	20.35	34
GCGTCAG	100	1.2852979E-8	20.35	11
GGACCGT	55	5.1372667E-4	20.181818	6
ACGTCAA	65	6.891731E-5	19.923077	29
CTAATAC	125	4.110916E-10	19.240002	3
CTGCGTC	135	5.638867E-11	19.185186	9
AACGTCA	80	1.6135655E-5	18.5	28
CGGGTAA	70	1.2173738E-4	18.5	23
GACCGTT	50	0.007029222	18.5	7
GCATTAC	50	0.007029222	18.5	1
GCGGGTA	70	1.2173738E-4	18.5	22
>>END_MODULE