FastQCFastQC Report
Fri 10 Feb 2017
ERR1632937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515959
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29790.5773714578096322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.43763167228403804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10980.2128076068059671No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.11609449588048663No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5540.10737287265073388No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGA250.00549357129.619
GCGCTCA250.00549357129.631
GACCGTT556.2505205E-726.909097
CTAGACA358.862742E-426.4285724
TAGGACC856.91216E-1126.1176474
TAGCGCT451.3218317E-424.66666629
GGACCGT701.9186518E-723.7857156
AGGACCG802.7184797E-823.1250025
ATCGCTC400.001929752423.1250028
CTAGCGC400.001929752423.12500228
GTATCAA15450.022.8705481
GGTATCA6100.022.7459011
TTTACCG705.092932E-621.14285930
CCGTTTA705.092932E-621.14285927
GTCTAGA450.003822993320.5555551
AGCGCTC450.003822993320.55555530
CTAGAGC555.1378156E-420.1818183
GCCGGTT656.892709E-519.92307722
CCGCCGT759.249548E-619.73333424
GCCGTTT951.6716513E-719.47368426
Produced by FastQC (version 0.11.5)