##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632937.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515959
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726900780876	33.0	33.0	33.0	27.0	33.0
2	31.253045300111054	33.0	33.0	33.0	27.0	33.0
3	31.662184398372737	33.0	33.0	33.0	27.0	33.0
4	31.78654699307503	33.0	33.0	33.0	27.0	33.0
5	31.904821894762957	33.0	33.0	33.0	33.0	33.0
6	34.73569411523009	37.0	37.0	37.0	27.0	37.0
7	34.876069610182206	37.0	37.0	37.0	27.0	37.0
8	35.08927065910276	37.0	37.0	37.0	33.0	37.0
9	35.14072048360432	37.0	37.0	37.0	33.0	37.0
10	35.203213821253236	37.0	37.0	37.0	33.0	37.0
11	35.24942291926296	37.0	37.0	37.0	33.0	37.0
12	35.15979564267703	37.0	37.0	37.0	33.0	37.0
13	35.21152455912195	37.0	37.0	37.0	33.0	37.0
14	35.17325407638979	37.0	37.0	37.0	33.0	37.0
15	35.2119742072529	37.0	37.0	37.0	33.0	37.0
16	35.20277580195326	37.0	37.0	37.0	33.0	37.0
17	35.18993175814357	37.0	37.0	37.0	33.0	37.0
18	35.17580272851137	37.0	37.0	37.0	33.0	37.0
19	35.20146755846879	37.0	37.0	37.0	33.0	37.0
20	35.13830750117742	37.0	37.0	37.0	33.0	37.0
21	35.19999263507372	37.0	37.0	37.0	33.0	37.0
22	35.217298273700045	37.0	37.0	37.0	33.0	37.0
23	35.18814867072771	37.0	37.0	37.0	33.0	37.0
24	35.17871381253162	37.0	37.0	37.0	33.0	37.0
25	35.19380609699608	37.0	37.0	37.0	33.0	37.0
26	35.154917348083856	37.0	37.0	37.0	33.0	37.0
27	35.0745640641989	37.0	37.0	37.0	33.0	37.0
28	35.07510674297764	37.0	37.0	37.0	33.0	37.0
29	35.10828573588211	37.0	37.0	37.0	33.0	37.0
30	35.06667390238372	37.0	37.0	37.0	27.0	37.0
31	35.05111840281883	37.0	37.0	37.0	27.0	37.0
32	35.07149792909902	37.0	37.0	37.0	33.0	37.0
33	35.03205874885408	37.0	37.0	37.0	27.0	37.0
34	34.97499413713105	37.0	37.0	37.0	27.0	37.0
35	34.972311753453276	37.0	37.0	37.0	27.0	37.0
36	34.94083250800936	37.0	37.0	37.0	27.0	37.0
37	34.95776990032154	37.0	37.0	37.0	27.0	37.0
38	34.81005661302545	37.0	37.0	37.0	27.0	37.0
39	34.848394930604954	37.0	37.0	37.0	27.0	37.0
40	34.8979957709818	37.0	37.0	37.0	27.0	37.0
41	34.907479082640286	37.0	37.0	37.0	27.0	37.0
42	34.758525386707085	37.0	37.0	37.0	27.0	37.0
43	34.56661866543659	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	8.0
16	25.0
17	27.0
18	20.0
19	14.0
20	43.0
21	90.0
22	301.0
23	742.0
24	1464.0
25	2700.0
26	4443.0
27	6586.0
28	9506.0
29	12885.0
30	16359.0
31	21007.0
32	26867.0
33	34844.0
34	49185.0
35	87748.0
36	241093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68199411193525	20.168656811878463	13.72841640517948	24.420932671006803
2	15.72508668324421	23.776113993553754	38.27668477534068	22.22211454786136
3	17.83998340953448	27.34577747456678	31.11797642835962	23.696262687539125
4	12.604877519337776	17.9178965770536	39.4637946038348	30.013431299773817
5	13.671628947261313	37.264976480689356	35.221403251033514	13.841991321015817
6	28.628825158588185	40.22315726637194	17.386846629286435	13.761170945753442
7	25.694677290249807	32.969286319261805	23.39391308224103	17.94212330824736
8	24.302318595082166	35.55767028000287	21.003800689589678	19.13621043532529
9	26.462761576016696	15.17736874441574	20.063222077723232	38.29664760184433
10	14.868817095932041	28.211156312807802	33.98312656625817	22.936900025001986
11	33.25768132739229	24.18040968371518	24.17847154521968	18.38343744367285
12	22.586484585015477	26.081335920102177	30.562118307850046	20.770061187032304
13	28.122777197412972	22.610129874660583	26.721309251316484	22.54578367660996
14	22.317277147990442	21.447052963510664	28.27201386156652	27.96365602693237
15	24.072649183365343	28.513505918105896	25.294839318628032	22.119005579900726
16	22.22308361710911	28.93853969016918	25.53904476906111	23.299331923660603
17	22.302353481575086	27.422140131289503	26.548233483668277	23.727272903467135
18	22.9744999118147	26.797090466490552	28.211737754356452	22.016671867338296
19	24.857401460193543	25.98055271833615	28.33752294271444	20.824522878755868
20	24.675022627766936	25.76154306834458	28.134793656085076	21.42864064780341
21	22.679127605100405	27.28976527204681	27.61130244845036	22.419804674402425
22	22.321153424981443	27.05990204648044	27.755306138666057	22.86363838987206
23	22.24866704524972	26.987415666748717	28.359423907713598	22.404493380287967
24	22.963064894691247	27.00350221626137	27.685145525128934	22.348287363918452
25	22.955893782257892	26.75910295197874	28.408264997800213	21.87673826796315
26	22.90937845836588	26.988578549846014	27.753368000170557	22.34867499161755
27	23.034388391325667	26.826937799321264	27.67816822654513	22.460505582807937
28	22.09167782711417	26.91047156847734	28.227242862320455	22.770607742088032
29	23.028573975839166	27.026566064357826	27.57098916774395	22.37387079205906
30	22.76479332660153	27.13258224006171	27.96094263303867	22.141681800298084
31	22.87875587013697	26.777515267686	27.92721902321696	22.41650983896007
32	21.61315143257507	26.798253349587853	28.230925325461907	23.357669892375167
33	22.06202430813301	26.611610612471146	28.62746846164133	22.69889661775451
34	22.119393207599828	26.56451384703048	28.29585296506118	23.020239980308514
35	22.368250190422106	27.326977531160423	28.06637736719391	22.238394911223565
36	22.63687618589849	27.06397213732099	27.965206537728772	22.333945139051746
37	22.941939185090288	26.746698865607538	27.592502505044003	22.718859444258168
38	22.87817442858832	26.83662849179877	27.66750846481988	22.617688614793035
39	22.304873061619237	26.824418219277113	27.98536317808198	22.88534554102167
40	22.207190881446007	27.018232068827174	28.221816074533052	22.552760975193763
41	22.37949139369601	26.38426696694892	28.389658868243405	22.84658277111166
42	22.27928963347863	26.87926753869978	28.025870272637942	22.81557255518365
43	22.325998771220192	26.425936944602185	28.164253361216684	23.083810922960932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	28.5
2	38.0
3	181.5
4	325.0
5	325.0
6	432.5
7	540.0
8	522.0
9	504.0
10	696.0
11	888.0
12	888.0
13	1313.0
14	1738.0
15	3080.0
16	4422.0
17	4855.0
18	5288.0
19	5288.0
20	4832.5
21	4377.0
22	4662.0
23	4947.0
24	6481.0
25	8015.0
26	8015.0
27	9731.0
28	11447.0
29	14513.5
30	17580.0
31	19787.0
32	21994.0
33	21994.0
34	24217.0
35	26440.0
36	28433.5
37	30427.0
38	32525.0
39	34623.0
40	34623.0
41	36223.5
42	37824.0
43	37411.0
44	36998.0
45	37306.5
46	37615.0
47	37615.0
48	37532.0
49	37449.0
50	38391.0
51	39333.0
52	39307.5
53	39282.0
54	39282.0
55	35009.0
56	30736.0
57	27601.0
58	24466.0
59	21564.0
60	18662.0
61	18662.0
62	16243.0
63	13824.0
64	11306.0
65	8788.0
66	7567.0
67	6346.0
68	6346.0
69	5207.5
70	4069.0
71	3506.5
72	2944.0
73	2448.0
74	1952.0
75	1952.0
76	1410.5
77	869.0
78	665.0
79	461.0
80	366.5
81	272.0
82	272.0
83	223.0
84	174.0
85	158.5
86	143.0
87	112.0
88	81.0
89	81.0
90	65.5
91	50.0
92	29.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	515959.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.40282231463977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.71577789401256	55.0880196884574
2	5.572812014796306	6.843727718748545
3	1.4742857645974623	2.715759205337424
4	0.7077098196243398	1.7382152121887637
5	0.43308597163080315	1.3296350481504662
6	0.31211503498197485	1.1498846420835467
7	0.21867650355291549	0.9399148144432453
8	0.1775893957755305	0.8723592091015311
9	0.12796868515327497	0.7071874592674162
>10	1.038645252889716	13.177178839045222
>50	0.1453297186909323	6.113972822297079
>100	0.07441390361858827	7.861993756357482
>500	6.360162702443441E-4	0.22514166358890736
>1k	9.540244053665162E-4	1.237009920932982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2979	0.5773714578096322	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2258	0.43763167228403804	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1098	0.2128076068059671	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.11609449588048663	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	554	0.10737287265073388	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.752553982002446E-4	0.0	0.0
2	0.0	0.0	0.001162883097300367	0.0	0.0
3	0.0	0.0	0.001162883097300367	1.9381384955006114E-4	0.0
4	0.0	0.0	0.0015505107964004891	3.876276991001223E-4	0.0
5	1.9381384955006114E-4	0.0	0.0015505107964004891	3.876276991001223E-4	0.0
6	1.9381384955006114E-4	0.0	0.004651532389201468	3.876276991001223E-4	0.0
7	1.9381384955006114E-4	0.0	0.008721623229752751	3.876276991001223E-4	0.0
8	5.814415486501835E-4	0.0	0.009690692477503057	5.814415486501835E-4	0.0
9	5.814415486501835E-4	0.0	0.012404086371203913	9.690692477503058E-4	0.0
10	0.002131952345050673	0.0	0.014536038716254586	9.690692477503058E-4	0.0
11	0.002131952345050673	0.0	0.023063848096457278	0.001162883097300367	0.0
12	0.002131952345050673	0.0	0.025583428140608072	0.001162883097300367	0.0
13	0.002131952345050673	0.0	0.02713393893700856	0.001162883097300367	0.0
14	0.002131952345050673	0.0	0.029847332830709415	0.001162883097300367	0.0
15	0.002131952345050673	0.0	0.03178547132621003	0.001356696946850428	0.0
16	0.002131952345050673	0.0	0.033529795972160575	0.0015505107964004891	0.0
17	0.002131952345050673	0.0	0.034498865219910885	0.0015505107964004891	0.0
18	0.002131952345050673	0.0	0.035855562166761314	0.0015505107964004891	0.0
19	0.002131952345050673	0.0	0.036437003715411496	0.0015505107964004891	0.0
20	0.002131952345050673	0.0	0.037793700662261925	0.0019381384955006115	0.0
21	0.002131952345050673	0.0	0.039538025308212474	0.002713393893700856	0.0
22	0.002131952345050673	0.0	0.04031328070641272	0.002907207743250917	0.0
23	0.002325766194600734	0.0	0.041088536104612966	0.0031010215928009783	0.0
24	0.002325766194600734	0.0	0.04128234995416302	0.003682463141451162	0.0
25	0.002325766194600734	0.0	0.04128234995416302	0.003682463141451162	0.0
26	0.002325766194600734	0.0	0.04128234995416302	0.003876276991001223	0.0
27	0.002325766194600734	0.0	0.041476163803713086	0.0062020431856019565	0.0
28	0.002325766194600734	0.0	0.041476163803713086	0.017830874158605625	0.0
29	0.002325766194600734	0.0	0.041476163803713086	0.044770999246064126	0.0
30	0.002325766194600734	0.0	0.04166997765326315	0.08043274756327537	0.0
31	0.002325766194600734	0.0	0.04166997765326315	0.18140976317885724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCGA	25	0.005493571	29.6	19
GCGCTCA	25	0.005493571	29.6	31
GACCGTT	55	6.2505205E-7	26.90909	7
CTAGACA	35	8.862742E-4	26.428572	4
TAGGACC	85	6.91216E-11	26.117647	4
TAGCGCT	45	1.3218317E-4	24.666666	29
GGACCGT	70	1.9186518E-7	23.785715	6
AGGACCG	80	2.7184797E-8	23.125002	5
ATCGCTC	40	0.0019297524	23.125002	8
CTAGCGC	40	0.0019297524	23.125002	28
GTATCAA	1545	0.0	22.870548	1
GGTATCA	610	0.0	22.745901	1
TTTACCG	70	5.092932E-6	21.142859	30
CCGTTTA	70	5.092932E-6	21.142859	27
GTCTAGA	45	0.0038229933	20.555555	1
AGCGCTC	45	0.0038229933	20.555555	30
CTAGAGC	55	5.1378156E-4	20.181818	3
GCCGGTT	65	6.892709E-5	19.923077	22
CCGCCGT	75	9.249548E-6	19.733334	24
GCCGTTT	95	1.6716513E-7	19.473684	26
>>END_MODULE