FastQCFastQC Report
Fri 10 Feb 2017
ERR1632936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489862
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14350.2929396442263331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.2047515422710886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5360.10941857094447008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2650.026.5283031
GTATCAA7900.023.417721
TATCGCG400.001929613823.1250027
TCTCGTC1200.023.12499812
CTTCTCG1250.022.19999910
ATCGCGC502.6990363E-422.1999998
TTCTCGT1151.3460522E-1020.91304411
AACTCGC450.003822721320.55555522
CGTCCGC909.4503775E-820.55555529
CGTCTTG1303.274181E-1119.92307515
TGTCCGG951.6712147E-719.47368414
TCGTCTT1355.638867E-1119.18518614
GTACATG1103.8382495E-818.51
CCGTCCG902.1470896E-618.528
GTACTAT701.2173853E-418.51
ATGCCCG1501.2732926E-1118.529
TAATATA1306.9303496E-1018.4999984
AATGCGT500.007029261418.4999986
TAGGGTC1306.9303496E-1018.4999984
TCGCGCG500.007029261418.4999989
Produced by FastQC (version 0.11.5)