FastQCFastQC Report
Fri 10 Feb 2017
ERR1632935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462391
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12040.260385690897963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.1996146118760962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4960.10726852382507446No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4670.10099677545626969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC754.6020432E-1027.1333314
GGTATCA2650.024.4339621
GTATCAA7050.023.8794331
TCTAGAC753.7307655E-722.1999993
TTACACC450.00382240220.5555554
AGCCTAA555.136704E-420.181826
TGCTCGC656.8907284E-519.92307910
AACGATT851.2420169E-619.58823422
TATACTG500.007028679418.4999985
TTAGGAC1258.538336E-917.7600023
ACGTGAT1054.781941E-717.6190537
TAACGGC953.5969624E-617.52631636
TTAACGG953.5969624E-617.52631635
AGCTTCG752.0639013E-417.26666621
ACGGGGT752.0639013E-417.26666616
CTCTAAT1301.3867975E-817.0769251
TTCAACG1005.8625174E-616.6519
ACGATTA1005.8625174E-616.6523
CTACGTG1005.8625174E-616.6535
ATCAACG10300.016.5242733
Produced by FastQC (version 0.11.5)