##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632935.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	462391
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.665854222941192	33.0	33.0	33.0	27.0	33.0
2	31.20477907225703	33.0	33.0	33.0	27.0	33.0
3	31.61630308548393	33.0	33.0	33.0	27.0	33.0
4	31.751935050638963	33.0	33.0	33.0	27.0	33.0
5	31.85761401065332	33.0	33.0	33.0	33.0	33.0
6	34.72950381819715	37.0	37.0	37.0	27.0	37.0
7	34.850338782545506	37.0	37.0	37.0	27.0	37.0
8	35.064880155539356	37.0	37.0	37.0	33.0	37.0
9	35.11842358523414	37.0	37.0	37.0	33.0	37.0
10	35.18335564489793	37.0	37.0	37.0	33.0	37.0
11	35.22335426078795	37.0	37.0	37.0	33.0	37.0
12	35.121031767486826	37.0	37.0	37.0	33.0	37.0
13	35.17962936129812	37.0	37.0	37.0	33.0	37.0
14	35.13327249016525	37.0	37.0	37.0	33.0	37.0
15	35.17058939295964	37.0	37.0	37.0	33.0	37.0
16	35.166826343938354	37.0	37.0	37.0	33.0	37.0
17	35.16115798101607	37.0	37.0	37.0	33.0	37.0
18	35.118384657140815	37.0	37.0	37.0	33.0	37.0
19	35.15806103492499	37.0	37.0	37.0	33.0	37.0
20	35.09555549307837	37.0	37.0	37.0	33.0	37.0
21	35.149384395457524	37.0	37.0	37.0	33.0	37.0
22	35.17080349747292	37.0	37.0	37.0	33.0	37.0
23	35.140136810621314	37.0	37.0	37.0	33.0	37.0
24	35.12534413515834	37.0	37.0	37.0	33.0	37.0
25	35.13577686416907	37.0	37.0	37.0	33.0	37.0
26	35.10081511102076	37.0	37.0	37.0	33.0	37.0
27	35.038744266216256	37.0	37.0	37.0	27.0	37.0
28	35.02643001269488	37.0	37.0	37.0	27.0	37.0
29	35.05205983680478	37.0	37.0	37.0	27.0	37.0
30	35.02268426504841	37.0	37.0	37.0	27.0	37.0
31	35.02285295345281	37.0	37.0	37.0	27.0	37.0
32	35.04584431790411	37.0	37.0	37.0	27.0	37.0
33	35.02096926627032	37.0	37.0	37.0	27.0	37.0
34	34.978509529813515	37.0	37.0	37.0	27.0	37.0
35	34.963783897177926	37.0	37.0	37.0	27.0	37.0
36	34.93469163543408	37.0	37.0	37.0	27.0	37.0
37	34.967713471931766	37.0	37.0	37.0	27.0	37.0
38	34.82371845472771	37.0	37.0	37.0	27.0	37.0
39	34.887389676702185	37.0	37.0	37.0	27.0	37.0
40	34.931049696036474	37.0	37.0	37.0	27.0	37.0
41	34.94832511878475	37.0	37.0	37.0	27.0	37.0
42	34.83283844192469	37.0	37.0	37.0	27.0	37.0
43	34.64046229273494	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	10.0
16	21.0
17	35.0
18	16.0
19	14.0
20	44.0
21	105.0
22	274.0
23	689.0
24	1411.0
25	2566.0
26	4132.0
27	6132.0
28	8749.0
29	11467.0
30	14882.0
31	19056.0
32	23953.0
33	31130.0
34	43787.0
35	79123.0
36	214793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90412659415949	20.478123492888052	13.226901042624101	25.39084887032836
2	16.324063400887994	22.790235969125696	37.37032078911571	23.515379840870605
3	17.70406430921017	26.566261021516425	30.156728829064576	25.572945840208828
4	12.750896968150332	17.69152081247256	38.92138904087666	30.63619317850045
5	13.998326091987085	36.907076478564676	35.59065812267107	13.503939306777166
6	29.977875866961078	39.83857817301807	17.223086089478386	12.960459870542461
7	26.88028097432692	32.2114833549961	23.597777638405592	17.310458032271388
8	24.840016349799196	35.7974095516565	20.451522629116916	18.911051469427388
9	25.920919741084923	15.225858634791766	20.387291275132952	38.465930348990355
10	14.787052516160564	28.361927459660762	34.151183738437815	22.699836285740858
11	34.3118702569903	23.569014102783143	24.02836560400181	18.090750036224755
12	23.062948889576138	25.674591417220494	30.329526309984406	20.932933383218963
13	27.966591045240936	22.196150011570296	26.097610031337116	23.739648911851656
14	22.02573146968691	20.702608831054235	27.465283710106814	29.80637598915204
15	23.672389817275853	28.820630159324033	24.17845503048286	23.32852499291725
16	23.16264806192162	28.41404785127738	24.98924070753972	23.434063379261275
17	22.685562651522197	27.537949484310897	26.41227878570301	23.364209078463897
18	23.308412144700046	27.313680413329845	27.318222024217597	22.059685417752508
19	24.564059421571788	26.46612931480068	26.912288517726342	22.057522745901196
20	24.112493539017844	26.478024009982892	27.15364269633276	22.255839754666507
21	23.24872240160384	27.005499674517885	27.0582688676899	22.687509056188375
22	23.057542209947858	26.8316208576724	26.948837672013514	23.161999260366226
23	22.97514441241287	26.696021332595144	27.49556111602518	22.833273138966806
24	23.12869411385602	27.362773064354627	26.32577191165053	23.18276091013882
25	23.44098392918547	26.633736383277355	27.239933303200104	22.685346384337066
26	23.166757138439113	27.059782737985817	27.013501560367743	22.759958563207327
27	23.076573722239406	26.978250009191356	26.6257344974275	23.31944177114174
28	22.95481529701054	26.59069921343625	27.47371813032693	22.98076735922628
29	23.818586434424546	26.752034533544123	26.684126637412923	22.745252394618408
30	23.329390061657772	26.817996025009137	27.040534958509138	22.812078954823946
31	23.463475716439117	26.657309506456656	27.03577708043625	22.84343769666797
32	22.54390764526126	26.69191225607765	27.231498882979988	23.5326812156811
33	22.755633219504706	26.598484832100972	27.69798720130798	22.947894747086337
34	23.28894809803824	26.236237297006216	27.417272395007686	23.057542209947858
35	22.994608459074676	27.060215272356082	27.16445605558932	22.780720212979926
36	23.77771193643475	26.712025104294852	26.691695988892516	22.818566970377884
37	22.9684401296738	26.39757261711409	27.04377896628611	23.590208286926
38	23.282676349669433	26.717648051108263	27.01501543066366	22.984660168558644
39	22.85771133088663	26.53511854685753	27.324061238216142	23.2831088840397
40	22.8505745137773	26.907530639653455	27.55092551541877	22.690969331150477
41	23.02207439158634	26.175249950799216	27.310652672738005	23.492022984876435
42	22.846032902889547	26.803073589235083	27.242528509421682	23.10836499845369
43	23.18211210858343	25.965470781221956	27.484747756768623	23.367669353425995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	14.0
2	22.0
3	85.5
4	149.0
5	149.0
6	179.5
7	210.0
8	196.0
9	182.0
10	246.0
11	310.0
12	310.0
13	489.0
14	668.0
15	1186.0
16	1704.0
17	1985.5
18	2267.0
19	2267.0
20	2292.5
21	2318.0
22	2808.5
23	3299.0
24	4495.0
25	5691.0
26	5691.0
27	7431.0
28	9171.0
29	11387.5
30	13604.0
31	16076.0
32	18548.0
33	18548.0
34	21454.5
35	24361.0
36	26598.0
37	28835.0
38	31366.0
39	33897.0
40	33897.0
41	35223.0
42	36549.0
43	37319.5
44	38090.0
45	37244.5
46	36399.0
47	36399.0
48	35312.5
49	34226.0
50	33893.5
51	33561.0
52	32011.5
53	30462.0
54	30462.0
55	28854.5
56	27247.0
57	24158.5
58	21070.0
59	19522.0
60	17974.0
61	17974.0
62	15985.5
63	13997.0
64	11812.5
65	9628.0
66	8198.0
67	6768.0
68	6768.0
69	5566.5
70	4365.0
71	3606.5
72	2848.0
73	2284.5
74	1721.0
75	1721.0
76	1324.5
77	928.0
78	712.0
79	496.0
80	389.5
81	283.0
82	283.0
83	242.0
84	201.0
85	186.0
86	171.0
87	134.5
88	98.0
89	98.0
90	74.0
91	50.0
92	32.0
93	14.0
94	8.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	462391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.50049032387977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34455422565493	59.63300727676294
2	6.33769556152532	8.555951158528712
3	1.8167159897961642	3.6788766027142095
4	0.9187566734819899	2.4806610379348415
5	0.5457836985307206	1.8420333630802113
6	0.3827452109001644	1.5501293642926721
7	0.26425362161122307	1.2486074320032985
8	0.2061506685585057	1.1132216966635808
9	0.16486437686911531	1.0015583648045567
>10	0.9141150166418008	11.408365987593024
>50	0.07311989245664022	3.354705438619672
>100	0.03060083585032139	3.6704117065485717
>500	3.2211406158233035E-4	0.2006865710055288
>1k	3.2211406158233035E-4	0.2617839994481654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1204	0.260385690897963	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.1996146118760962	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	496	0.10726852382507446	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	467	0.10099677545626969	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.488015553935954E-4	0.0	0.0
2	2.1626718513119848E-4	0.0	6.488015553935954E-4	0.0	0.0
3	2.1626718513119848E-4	0.0	6.488015553935954E-4	0.0	0.0
4	2.1626718513119848E-4	0.0	6.488015553935954E-4	2.1626718513119848E-4	0.0
5	2.1626718513119848E-4	0.0	6.488015553935954E-4	2.1626718513119848E-4	0.0
6	2.1626718513119848E-4	0.0	0.0010813359256559925	2.1626718513119848E-4	0.0
7	2.1626718513119848E-4	0.0	0.0025952062215743817	4.3253437026239696E-4	0.0
8	2.1626718513119848E-4	0.0	0.003027740591836779	4.3253437026239696E-4	0.0
9	2.1626718513119848E-4	0.0	0.004109076517492771	4.3253437026239696E-4	0.0
10	6.488015553935954E-4	0.0	0.00432534370262397	4.3253437026239696E-4	0.0
11	8.650687405247939E-4	0.0	0.006704282739067153	4.3253437026239696E-4	0.0
12	8.650687405247939E-4	0.0	0.007785618664723146	4.3253437026239696E-4	0.0
13	8.650687405247939E-4	0.0	0.00865068740524794	4.3253437026239696E-4	0.0
14	8.650687405247939E-4	0.0	0.009299488960641535	4.3253437026239696E-4	0.0
15	8.650687405247939E-4	0.0	0.009515756145772733	6.488015553935954E-4	0.0
16	8.650687405247939E-4	0.0	0.011029626441691124	6.488015553935954E-4	0.0
17	8.650687405247939E-4	0.0	0.011462160811953519	6.488015553935954E-4	0.0
18	8.650687405247939E-4	0.0	0.011894695182215916	6.488015553935954E-4	0.0
19	8.650687405247939E-4	0.0	0.012110962367347116	8.650687405247939E-4	2.1626718513119848E-4
20	8.650687405247939E-4	0.0	0.012543496737609513	8.650687405247939E-4	2.1626718513119848E-4
21	8.650687405247939E-4	0.0	0.01275976392274071	8.650687405247939E-4	2.1626718513119848E-4
22	8.650687405247939E-4	0.0	0.01275976392274071	0.0010813359256559925	2.1626718513119848E-4
23	8.650687405247939E-4	0.0	0.01275976392274071	0.0012976031107871909	2.1626718513119848E-4
24	8.650687405247939E-4	0.0	0.01275976392274071	0.0012976031107871909	2.1626718513119848E-4
25	8.650687405247939E-4	0.0	0.01275976392274071	0.0012976031107871909	2.1626718513119848E-4
26	8.650687405247939E-4	0.0	0.01275976392274071	0.0012976031107871909	2.1626718513119848E-4
27	8.650687405247939E-4	0.0	0.01275976392274071	0.0017301374810495878	2.1626718513119848E-4
28	8.650687405247939E-4	0.0	0.01275976392274071	0.009083221775510337	2.1626718513119848E-4
29	8.650687405247939E-4	0.0	0.01275976392274071	0.023356855994169437	2.1626718513119848E-4
30	8.650687405247939E-4	0.0	0.01275976392274071	0.049308918209913254	2.1626718513119848E-4
31	8.650687405247939E-4	0.0	0.01275976392274071	0.11245893626822322	2.1626718513119848E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	75	4.6020432E-10	27.133331	4
GGTATCA	265	0.0	24.433962	1
GTATCAA	705	0.0	23.879433	1
TCTAGAC	75	3.7307655E-7	22.199999	3
TTACACC	45	0.003822402	20.555555	4
AGCCTAA	55	5.136704E-4	20.18182	6
TGCTCGC	65	6.8907284E-5	19.923079	10
AACGATT	85	1.2420169E-6	19.588234	22
TATACTG	50	0.0070286794	18.499998	5
TTAGGAC	125	8.538336E-9	17.760002	3
ACGTGAT	105	4.781941E-7	17.61905	37
TAACGGC	95	3.5969624E-6	17.526316	36
TTAACGG	95	3.5969624E-6	17.526316	35
AGCTTCG	75	2.0639013E-4	17.266666	21
ACGGGGT	75	2.0639013E-4	17.266666	16
CTCTAAT	130	1.3867975E-8	17.076925	1
TTCAACG	100	5.8625174E-6	16.65	19
ACGATTA	100	5.8625174E-6	16.65	23
CTACGTG	100	5.8625174E-6	16.65	35
ATCAACG	1030	0.0	16.524273	3
>>END_MODULE