FastQCFastQC Report
Fri 10 Feb 2017
ERR1632934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431121
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8730.20249535513231784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.1551768529020855No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5290.12270337097937702No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5150.11945602278710618No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4720.10948202476798857No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4700.10901811788337845No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4480.1039151421526671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2500.025.161
ATCGCTA502.6983078E-422.210
GTATCAA6100.021.5327871
GTACCAT450.00382198820.5555576
TAGACTC555.135933E-420.1818185
CTAGACT656.889348E-519.9230774
CGGAGTT656.889348E-519.92307724
CATGCTA902.1458727E-618.54
ATAGCAC500.00702792618.53
GCACGGA701.216956E-418.521
GGCATGC1409.276846E-1118.529
TAGGACC902.1458727E-618.54
TTATACT1552.0008883E-1117.9032274
GCACCGC1351.1459633E-917.81481410
AGTTAGC852.7167289E-517.41176427
CCGTATT752.0634927E-417.2666661
GGCACCG1401.8571882E-917.1785729
TTAGCCG650.001577769317.07692329
TTTCGGG650.001577769317.07692322
CGCTATC650.001577769317.07692312
Produced by FastQC (version 0.11.5)