##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632934.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431121
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53289447742049	33.0	33.0	33.0	27.0	33.0
2	31.061242667371804	33.0	33.0	33.0	27.0	33.0
3	31.505097176894655	33.0	33.0	33.0	27.0	33.0
4	31.65258013411548	33.0	33.0	33.0	27.0	33.0
5	31.783749805738992	33.0	33.0	33.0	33.0	33.0
6	34.627232261940385	37.0	37.0	37.0	27.0	37.0
7	34.82252546268913	37.0	37.0	37.0	27.0	37.0
8	34.993442676186035	37.0	37.0	37.0	27.0	37.0
9	35.08515242820461	37.0	37.0	37.0	33.0	37.0
10	35.11709473674444	37.0	37.0	37.0	33.0	37.0
11	35.13645357103922	37.0	37.0	37.0	33.0	37.0
12	35.08973814775898	37.0	37.0	37.0	33.0	37.0
13	35.113518014664095	37.0	37.0	37.0	33.0	37.0
14	35.09757353504005	37.0	37.0	37.0	33.0	37.0
15	35.10187163232596	37.0	37.0	37.0	33.0	37.0
16	35.0900420067684	37.0	37.0	37.0	33.0	37.0
17	35.06122875016527	37.0	37.0	37.0	33.0	37.0
18	35.0824548096706	37.0	37.0	37.0	33.0	37.0
19	35.0955161080068	37.0	37.0	37.0	33.0	37.0
20	35.09363032652086	37.0	37.0	37.0	33.0	37.0
21	35.081536274039074	37.0	37.0	37.0	33.0	37.0
22	35.09367671720932	37.0	37.0	37.0	33.0	37.0
23	35.08304629094848	37.0	37.0	37.0	33.0	37.0
24	35.0869222329694	37.0	37.0	37.0	33.0	37.0
25	35.07564697613895	37.0	37.0	37.0	33.0	37.0
26	35.01252316635005	37.0	37.0	37.0	27.0	37.0
27	34.95404538401052	37.0	37.0	37.0	27.0	37.0
28	34.938467390825316	37.0	37.0	37.0	27.0	37.0
29	34.98169655386771	37.0	37.0	37.0	27.0	37.0
30	34.933422403455175	37.0	37.0	37.0	27.0	37.0
31	34.91948664064149	37.0	37.0	37.0	27.0	37.0
32	34.94800067730405	37.0	37.0	37.0	27.0	37.0
33	34.93218609160769	37.0	37.0	37.0	27.0	37.0
34	34.87332790562278	37.0	37.0	37.0	27.0	37.0
35	34.85553011799472	37.0	37.0	37.0	27.0	37.0
36	34.872298032338946	37.0	37.0	37.0	27.0	37.0
37	34.860565827227155	37.0	37.0	37.0	27.0	37.0
38	34.719746892403755	37.0	37.0	37.0	27.0	37.0
39	34.81481533026691	37.0	37.0	37.0	27.0	37.0
40	34.84398811470562	37.0	37.0	37.0	27.0	37.0
41	34.85790764077834	37.0	37.0	37.0	27.0	37.0
42	34.7765754857685	37.0	37.0	37.0	27.0	37.0
43	34.53291999229915	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	8.0
16	14.0
17	28.0
18	12.0
19	23.0
20	36.0
21	104.0
22	279.0
23	673.0
24	1371.0
25	2504.0
26	4072.0
27	6256.0
28	8552.0
29	11128.0
30	14658.0
31	18277.0
32	23518.0
33	30036.0
34	41680.0
35	74763.0
36	193126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.82844491453675	20.434402406748916	13.008644904794709	25.728507773919617
2	16.66724655027243	22.692237214146378	36.62660830718058	24.01390792840061
3	17.728201595375776	26.508335246949233	29.815527427334786	25.947935730340205
4	12.824473871604491	17.602018922761822	38.99114169803837	30.582365507595316
5	14.073311204974937	36.91724597038883	35.618770600365096	13.390672224271144
6	30.354819180694054	39.63620422108875	17.020975549787646	12.98800104842956
7	27.267054956729087	31.48373658439278	23.82764931422965	17.421559144648484
8	24.89254756785218	35.665161288826106	20.46664393522932	18.97564720809239
9	25.996182046339662	15.047747616098498	19.893023072408905	39.06304726515294
10	14.905560155965494	27.947374403009828	34.287357841534046	22.85970759949063
11	34.602118662742015	23.261914868447604	23.730924728788438	18.405041740021943
12	22.94645818691272	25.548975809575502	30.041450080139914	21.463115923371863
13	27.928586174183117	21.730094335464987	25.874406489129502	24.466913001222395
14	22.09333342611471	20.44692789263339	27.073373832404357	30.386364848847542
15	23.981202493035596	28.683826582328397	23.863138190902323	23.471832733733685
16	23.613324333539772	27.864567024106922	24.943113418274685	23.57899522407862
17	22.864114714894427	27.39022223459307	25.95535824049397	23.790304810018533
18	23.119263501429995	27.275405280652066	27.25197798297926	22.353353234938684
19	24.648068639662647	26.13326653074195	26.803611979003573	22.41505285059183
20	24.08813302993823	26.399317129065853	26.99404575513603	22.518504085859885
21	23.182586791179276	26.961340319771015	26.75954082496561	23.096532064084098
22	23.14663400762199	26.69134651292793	26.6672233549282	23.494796124521887
23	23.041791051700102	26.672558284101218	27.314141505517014	22.97150915868167
24	23.35840750044651	27.251514076094647	25.638509838305257	23.75156858515359
25	23.58154671194398	26.54753537869879	27.04901872096233	22.821899188394905
26	23.400854980388335	26.996365289559083	26.76441184725402	22.838367882798565
27	23.42080297642657	26.833533973060923	26.117493696665207	23.628169353847294
28	22.891485221086423	26.636373547101627	27.24293179872936	23.22920943308259
29	24.276247271647637	26.5892869983137	26.429471076565513	22.704994653473154
30	23.619123169597398	26.515757757102993	26.703176138485485	23.161942934814125
31	23.491780729771918	26.508799153833845	26.898480936906346	23.10093917948789
32	22.72192725476142	26.531762544622044	26.778329053792323	23.96798114682421
33	22.843006951644664	26.59021481208292	27.340120291055182	23.226657945217237
34	23.6237622384435	25.996877906666572	27.077780947808154	23.301578907081772
35	23.330341133927597	27.026055330174124	26.633822059236266	23.009781476662006
36	24.04267015524644	26.65492982248603	26.27939719939414	23.02300282287339
37	23.209261437044358	26.101952816030767	26.702480278158568	23.986305468766307
38	23.50708965696405	26.82402388192642	26.28705166299021	23.38183479811932
39	22.8536768099907	26.41694559068104	27.186335158806923	23.54304244052134
40	23.12274280306457	26.89732116969482	27.200716272229837	22.779219755010775
41	23.138979544025922	26.024016459416266	27.062240067173715	23.774763929384093
42	22.693165027915597	26.97479361942471	26.931650279155967	23.400391073503727
43	23.542346580194423	25.836134171149165	26.979896595155424	23.641622653500992
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	7.5
2	11.0
3	62.5
4	114.0
5	114.0
6	134.5
7	155.0
8	137.0
9	119.0
10	178.5
11	238.0
12	238.0
13	370.5
14	503.0
15	915.5
16	1328.0
17	1491.0
18	1654.0
19	1654.0
20	1609.0
21	1564.0
22	1881.5
23	2199.0
24	3123.0
25	4047.0
26	4047.0
27	5505.0
28	6963.0
29	8869.0
30	10775.0
31	13236.5
32	15698.0
33	15698.0
34	18668.0
35	21638.0
36	24226.5
37	26815.0
38	29443.0
39	32071.0
40	32071.0
41	34028.0
42	35985.0
43	36907.5
44	37830.0
45	36884.5
46	35939.0
47	35939.0
48	34332.0
49	32725.0
50	32627.0
51	32529.0
52	30482.0
53	28435.0
54	28435.0
55	27461.5
56	26488.0
57	23173.5
58	19859.0
59	18477.5
60	17096.0
61	17096.0
62	15132.5
63	13169.0
64	11092.0
65	9015.0
66	7526.0
67	6037.0
68	6037.0
69	4998.5
70	3960.0
71	3259.0
72	2558.0
73	2039.0
74	1520.0
75	1520.0
76	1222.5
77	925.0
78	721.0
79	517.0
80	408.0
81	299.0
82	299.0
83	243.0
84	187.0
85	138.0
86	89.0
87	63.5
88	38.0
89	38.0
90	30.0
91	22.0
92	12.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	431121.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.159114392757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43640956405152	54.34969784183831
2	6.72335991091946	8.358361956130384
3	2.0127391579211267	3.753302506800021
4	0.9950640171109021	2.4740919227085145
5	0.5515927680555228	1.714325898389036
6	0.3803398528386966	1.4184953052434943
7	0.26446653755298644	1.1507304032568715
8	0.21674459398658358	1.0778121609298976
9	0.16709373996816412	0.9347759007295234
>10	1.0756844959323013	12.786685039470278
>50	0.11491625674770559	4.908425203383813
>100	0.06008693162441594	6.4696344936119505
>500	0.0015021732906103987	0.6036613675078659
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	873	0.20249535513231784	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	669	0.1551768529020855	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	529	0.12270337097937702	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	515	0.11945602278710618	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	472	0.10948202476798857	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	470	0.10901811788337845	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	448	0.1039151421526671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.3195344230506052E-4	0.0	0.0
4	0.0	0.0	4.6390688461012104E-4	2.3195344230506052E-4	0.0
5	0.0	0.0	4.6390688461012104E-4	2.3195344230506052E-4	0.0
6	0.0	0.0	6.958603269151816E-4	2.3195344230506052E-4	0.0
7	0.0	0.0	0.0020875809807455446	2.3195344230506052E-4	0.0
8	0.0	0.0	0.0023195344230506052	2.3195344230506052E-4	0.0
9	0.0	0.0	0.0027834413076607265	4.6390688461012104E-4	0.0
10	0.0	0.0	0.0027834413076607265	4.6390688461012104E-4	0.0
11	0.0	0.0	0.004175161961491089	4.6390688461012104E-4	0.0
12	0.0	0.0	0.00440711540379615	4.6390688461012104E-4	0.0
13	0.0	0.0	0.004871022288406271	4.6390688461012104E-4	0.0
14	0.0	0.0	0.005334929173016392	4.6390688461012104E-4	0.0
15	0.0	0.0	0.0060307894999315735	6.958603269151816E-4	0.0
16	0.0	0.0	0.006494696384541695	9.278137692202421E-4	0.0
17	0.0	0.0	0.006958603269151816	9.278137692202421E-4	0.0
18	0.0	0.0	0.007190556711456877	9.278137692202421E-4	0.0
19	0.0	0.0	0.007190556711456877	9.278137692202421E-4	0.0
20	0.0	0.0	0.007654463596066997	0.0013917206538303633	0.0
21	0.0	0.0	0.007886417038372059	0.0013917206538303633	0.0
22	0.0	0.0	0.008350323922982178	0.0013917206538303633	0.0
23	0.0	0.0	0.008350323922982178	0.0016236740961354237	0.0
24	0.0	0.0	0.008350323922982178	0.0020875809807455446	0.0
25	0.0	0.0	0.008350323922982178	0.0023195344230506052	0.0
26	0.0	0.0	0.008350323922982178	0.002551487865355666	0.0
27	0.0	0.0	0.008350323922982178	0.0030153947499657868	0.0
28	0.0	0.0	0.008350323922982178	0.009510091134507482	0.0
29	0.0	0.0	0.008350323922982178	0.025050971768946537	0.0
30	0.0	0.0	0.008350323922982178	0.04801436255714753	0.0
31	0.0	0.0	0.008350323922982178	0.11203351263334424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	250	0.0	25.16	1
ATCGCTA	50	2.6983078E-4	22.2	10
GTATCAA	610	0.0	21.532787	1
GTACCAT	45	0.003821988	20.555557	6
TAGACTC	55	5.135933E-4	20.181818	5
CTAGACT	65	6.889348E-5	19.923077	4
CGGAGTT	65	6.889348E-5	19.923077	24
CATGCTA	90	2.1458727E-6	18.5	4
ATAGCAC	50	0.007027926	18.5	3
GCACGGA	70	1.216956E-4	18.5	21
GGCATGC	140	9.276846E-11	18.5	29
TAGGACC	90	2.1458727E-6	18.5	4
TTATACT	155	2.0008883E-11	17.903227	4
GCACCGC	135	1.1459633E-9	17.814814	10
AGTTAGC	85	2.7167289E-5	17.411764	27
CCGTATT	75	2.0634927E-4	17.266666	1
GGCACCG	140	1.8571882E-9	17.178572	9
TTAGCCG	65	0.0015777693	17.076923	29
TTTCGGG	65	0.0015777693	17.076923	22
CGCTATC	65	0.0015777693	17.076923	12
>>END_MODULE