FastQCFastQC Report
Fri 10 Feb 2017
ERR1632933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374724
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121853.2517266041139616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT94472.5210554968456784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48871.3041598616581804No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.6943777286749714No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG25040.6682251470415559No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG17860.4766174571151034No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13920.3714734044256573No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13870.3701390890361973No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.3640012382446814No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12630.3370480673775899No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10950.2922150702917347No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10420.27807132716345895No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10320.275402696384539No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9320.2487163885953395No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9300.2481826624395555No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8790.23457264546706377No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8250.220162039260896No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8150.21749340848197604No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.2108218315346762No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7800.20815320075575622No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7700.20548456997683626No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7470.1993467191853204No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7370.19667808840640044No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7320.19534377301694048No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG7170.19134082684856055No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.18973964838120858No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6920.18466924990126066No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6860.18306807143390869No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6830.1822674822002327No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6640.17719708372028478No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6510.17372786370768886No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6460.1723935483182289No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6440.1718598221624449No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6390.17052550677298492No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6270.16732314983828098No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6110.16305334059200904No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6100.16278647751411707No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6090.16251961443622506No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6000.1601178467351971No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5890.15718235287838514No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5880.15691548980049316No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5870.15664862672260116No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5770.15397999594368122No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5770.15397999594368122No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG5710.15237881747632925No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5640.15051077593108528No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5490.14650782976270535No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5480.14624096668481334No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5360.1430386097501094No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5300.14143743128275746No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG5220.13930252665962148No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5210.13903566358172947No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5170.1379682112701615No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5130.13690075895859352No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5080.13556644356913355No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5010.13369840202388958No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5010.13369840202388958No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4900.13076290816707764No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4770.12729368815448172No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4710.12569250968712972No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4690.12515878353134574No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4690.12515878353134574No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.12489192045345374No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4630.12355760506399377No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4530.12088897428507381No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG4490.11982152197350583No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4480.11955465889561384No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4410.11768661735036988No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4400.11741975427247789No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4390.11715289119458588No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.11688602811669388No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4240.11314994502620596No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.11314994502620596No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4200.11208249271463797No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4140.110481314247286No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4140.110481314247286No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4130.110214451169394No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4080.10888013577993404No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4080.10888013577993404No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4050.10807954654625804No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3930.10487718961155411No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3910.10434346345577011No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3900.10407660037787811No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3840.10247542191052614No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3830.10220855883263415No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3800.10140796959895818No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3780.10087424344317418No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3760.10034051728739019No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3750.10007365420949818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCAA251.2320382E-437.011
GTCCAAA303.59489E-430.83333412
AGTGCAA900.030.83333211
CTAGTGC900.030.8333329
TAGTGCA900.030.83333210
CTTCCCT250.005491675329.635
GGATAGT250.005491675329.619
AAGCAAG250.005491675329.632
GATATCA250.005491675329.61
GAATCAA702.055458E-1029.071431
ACTAGTG1900.028.2368438
GGTATCA18400.026.6440221
GTATTAT1050.026.428571
TATTATA1100.025.2272722
TCCCTGT451.3208883E-424.66666637
TATTAGC451.3208883E-424.6666662
TGACATC1300.024.19230817
ATTATAG1150.024.1304363
GTGACAT1150.024.13043616
GCAGCTT6200.023.87096819
Produced by FastQC (version 0.11.5)