FastQCFastQC Report
Fri 10 Feb 2017
ERR1632932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301683
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6540.21678384264277403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.17999025467129404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTT358.8541757E-426.4285732
CATACCC358.8541757E-426.4285730
GTATCAA4050.025.1234571
GGCCGTG502.6957053E-422.19999911
ACGGGAC502.6957053E-422.19999916
GGTATCA1550.021.483871
TGTATCG450.003819367620.5555555
TGGCCGT450.003819367620.55555510
GTATCGC450.003819367620.5555556
GTTTAGG555.1310175E-420.1818181
CCTATAG656.880584E-519.9230772
ATCAACG5700.018.824563
TCAACGC5700.018.824564
AACGCAG5850.018.6581216
CAACGCA5900.018.55
GCGGCCG801.6102385E-518.512
GGACAGT701.2154186E-418.56
TATACAC801.6102385E-518.53
AGTACCG701.2154186E-418.55
TAGTACC609.215304E-418.54
Produced by FastQC (version 0.11.5)