##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632932.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301683
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91494051703278	33.0	33.0	33.0	27.0	33.0
2	30.651120547064302	33.0	33.0	33.0	27.0	33.0
3	31.090787349635214	33.0	33.0	33.0	27.0	33.0
4	31.250991935243285	33.0	33.0	33.0	27.0	33.0
5	31.387174617064932	33.0	33.0	33.0	27.0	33.0
6	33.9846925415088	37.0	33.0	37.0	27.0	37.0
7	34.19335527689661	37.0	33.0	37.0	27.0	37.0
8	34.379921971075596	37.0	37.0	37.0	27.0	37.0
9	34.46805753058674	37.0	37.0	37.0	27.0	37.0
10	34.54255625938485	37.0	37.0	37.0	27.0	37.0
11	34.47891661114481	37.0	37.0	37.0	27.0	37.0
12	34.50331970976157	37.0	37.0	37.0	27.0	37.0
13	34.50369096037894	37.0	37.0	37.0	27.0	37.0
14	34.524351720183105	37.0	37.0	37.0	27.0	37.0
15	34.491317044712495	37.0	37.0	37.0	27.0	37.0
16	34.50769847820394	37.0	37.0	37.0	27.0	37.0
17	34.481008210605175	37.0	37.0	37.0	27.0	37.0
18	34.46749071044772	37.0	37.0	37.0	27.0	37.0
19	34.48942432951144	37.0	37.0	37.0	27.0	37.0
20	34.50162256408217	37.0	37.0	37.0	27.0	37.0
21	34.4927854734937	37.0	37.0	37.0	27.0	37.0
22	34.49425058753725	37.0	37.0	37.0	27.0	37.0
23	34.47304289602	37.0	37.0	37.0	27.0	37.0
24	34.45603829184939	37.0	37.0	37.0	27.0	37.0
25	34.445891879887164	37.0	37.0	37.0	27.0	37.0
26	34.41970876714962	37.0	37.0	37.0	27.0	37.0
27	34.32632266319282	37.0	37.0	37.0	27.0	37.0
28	34.2893699678139	37.0	37.0	37.0	27.0	37.0
29	34.337065727932966	37.0	37.0	37.0	27.0	37.0
30	34.29208142321576	37.0	37.0	37.0	27.0	37.0
31	34.291004133477855	37.0	37.0	37.0	27.0	37.0
32	34.30942744536484	37.0	37.0	37.0	27.0	37.0
33	34.281623425913956	37.0	37.0	37.0	27.0	37.0
34	34.20636230745518	37.0	37.0	37.0	27.0	37.0
35	34.20338898777856	37.0	37.0	37.0	27.0	37.0
36	34.1663799418595	37.0	37.0	37.0	27.0	37.0
37	34.20990907674612	37.0	37.0	37.0	27.0	37.0
38	34.074429119307354	37.0	37.0	37.0	27.0	37.0
39	34.134495480355206	37.0	37.0	37.0	27.0	37.0
40	34.178448901661675	37.0	37.0	37.0	27.0	37.0
41	34.17664236963965	37.0	37.0	37.0	27.0	37.0
42	34.097509637599735	37.0	37.0	37.0	27.0	37.0
43	33.853276452435175	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	14.0
17	12.0
18	19.0
19	18.0
20	72.0
21	186.0
22	421.0
23	985.0
24	1855.0
25	3061.0
26	4450.0
27	6315.0
28	8452.0
29	10662.0
30	13455.0
31	15974.0
32	19719.0
33	24828.0
34	33446.0
35	55663.0
36	102069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.209425125048476	20.950799348985523	13.2082351342303	25.631540391735697
2	16.23127587567082	23.517732189085894	36.37029597292522	23.880695962318065
3	18.314919965659318	26.777113725334207	30.070305585664425	24.83766072334205
4	13.467116145092698	18.372264927092345	37.956066467119456	30.2045524606955
5	14.676000967903397	36.810824607286456	35.0589857565723	13.454188668237851
6	30.183669613468446	39.72746226999864	17.21840474935611	12.870463367176804
7	26.703858023156755	32.024674907104476	23.546238932919657	17.725228136819112
8	24.82705356284577	36.02954094198215	20.661091277930808	18.482314217241278
9	25.821806333137765	15.404248830726292	19.920578885784085	38.85336595035186
10	14.573575574361167	28.580993957233254	34.468962453966576	22.376468014438995
11	34.5299536268202	23.255204966802903	23.928428184551333	18.28641322182556
12	22.523642366324918	25.5115468886215	30.718005323468674	21.24680542158491
13	28.165325855285182	21.944557697980994	26.042236387201136	23.847880059532688
14	21.68236194946351	20.777770043389914	27.514642853591354	30.025225153555223
15	23.6118707384904	28.962851735099427	23.795175730816783	23.63010179559339
16	23.248575491492726	28.333714528163668	24.960306016580315	23.457403963763287
17	22.472263932671048	27.876612205526996	26.578892413559927	23.07223144824203
18	23.06692786799389	27.641928779546742	27.193776248578803	22.097367103880565
19	24.207529095109766	26.817553524726286	26.87324111733177	22.10167626283218
20	23.688109704557434	26.978318300998065	26.91832154944097	22.41525044500353
21	23.13388556862667	27.09632296151921	27.07411421923012	22.695677250623998
22	22.97477816118243	26.96936850932933	26.88086501393847	23.174988315549765
23	22.86804360868859	26.814238787071197	27.432437359745165	22.88528024449505
24	23.090462505345013	27.141734867393918	26.42409416506731	23.34370846219376
25	23.413317952950614	26.78871530712702	26.988593987728844	22.80937275219353
26	23.108030614916984	27.134442444552725	27.012791572610983	22.744735367919304
27	23.118637775413266	27.126818547946023	26.70054328550167	23.05400039113904
28	22.78351779848384	27.023067259341758	27.231232784081307	22.962182158093096
29	23.64733843139985	26.989588409025366	26.679328964509104	22.68374419506568
30	23.338736355711127	26.846391742325554	26.858987745414893	22.955884156548432
31	23.245260753837638	26.79667067749923	26.985610723839258	22.97245784482387
32	22.63667492036343	26.941856186792094	27.0432871590378	23.378181733806677
33	22.651922713576834	26.84407142596699	27.48613610975759	23.01786975069858
34	23.194213793949277	26.709824550935917	27.071462429106045	23.024499226008757
35	22.858099395723325	27.30879764521037	27.021409890514214	22.81169306855209
36	23.482595969941958	27.010802730017936	26.578892413559927	22.92770888648018
37	22.960193315500042	26.729712976866445	26.856004481525307	23.4540892261082
38	23.075877659662623	26.976992405936034	26.835121634298254	23.11200830010309
39	22.749376000636428	27.025056101934812	27.34028765293371	22.88528024449505
40	23.12062661800632	26.999532621990635	27.210018463088737	22.66982229691431
41	22.918427621045932	26.419785006115692	27.185820878206595	23.475966494631784
42	22.677777667286524	26.958429875067537	27.13510539208374	23.228687065562195
43	23.15642578468127	26.237474435085833	27.250126788715306	23.355972991517586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	11.5
2	14.0
3	46.5
4	79.0
5	79.0
6	97.5
7	116.0
8	118.5
9	121.0
10	186.0
11	251.0
12	251.0
13	332.0
14	413.0
15	806.5
16	1200.0
17	1341.5
18	1483.0
19	1483.0
20	1645.0
21	1807.0
22	2140.0
23	2473.0
24	3194.0
25	3915.0
26	3915.0
27	5075.0
28	6235.0
29	7537.0
30	8839.0
31	10509.5
32	12180.0
33	12180.0
34	13983.0
35	15786.0
36	17177.5
37	18569.0
38	20015.0
39	21461.0
40	21461.0
41	22376.0
42	23291.0
43	23576.5
44	23862.0
45	23913.5
46	23965.0
47	23965.0
48	23161.0
49	22357.0
50	22404.0
51	22451.0
52	21381.5
53	20312.0
54	20312.0
55	19193.0
56	18074.0
57	16091.5
58	14109.0
59	13069.0
60	12029.0
61	12029.0
62	10439.0
63	8849.0
64	7442.0
65	6035.0
66	5173.5
67	4312.0
68	4312.0
69	3581.5
70	2851.0
71	2304.0
72	1757.0
73	1419.0
74	1081.0
75	1081.0
76	855.5
77	630.0
78	482.0
79	334.0
80	264.0
81	194.0
82	194.0
83	150.0
84	106.0
85	92.5
86	79.0
87	57.5
88	36.0
89	36.0
90	25.5
91	15.0
92	9.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	301683.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.43074790561029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.73922841308193	65.1618791093939
2	6.390804827664449	9.385631564283706
3	1.9120842887723069	4.212173381493522
4	0.8765334292751275	2.5745802110376785
5	0.5263324750998085	1.932449364679497
6	0.3263432531562899	1.4378177491929771
7	0.2096884698852652	1.0778306819600645
8	0.16021174081581147	0.941157436109186
9	0.1258821268664316	0.8319256851375892
>10	0.684755199381976	9.102313572732909
>50	0.035874776453295586	1.7766598172125405
>100	0.011352777358636214	1.1664331908049501
>500	9.08222188690897E-4	0.3991482359615567
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	654	0.21678384264277403	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	543	0.17999025467129404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.314737655088288E-4	0.0	3.314737655088288E-4	0.0	0.0
3	3.314737655088288E-4	0.0	6.629475310176576E-4	0.0	0.0
4	3.314737655088288E-4	0.0	6.629475310176576E-4	0.0	0.0
5	3.314737655088288E-4	0.0	9.944212965264864E-4	0.0	0.0
6	3.314737655088288E-4	0.0	0.0013258950620353151	0.0	0.0
7	3.314737655088288E-4	0.0	0.004640632717123603	0.0	0.0
8	3.314737655088288E-4	0.0	0.005966527779158918	0.0	0.0
9	3.314737655088288E-4	0.0	0.006960949075685405	3.314737655088288E-4	0.0
10	9.944212965264864E-4	0.0	0.008618317903229549	3.314737655088288E-4	0.0
11	9.944212965264864E-4	0.0	0.010275686730773692	3.314737655088288E-4	0.0
12	9.944212965264864E-4	0.0	0.010607160496282521	3.314737655088288E-4	0.0
13	9.944212965264864E-4	0.0	0.011270108027300179	3.314737655088288E-4	0.0
14	9.944212965264864E-4	0.0	0.012264529323826667	3.314737655088288E-4	0.0
15	9.944212965264864E-4	0.0	0.012596003089335495	3.314737655088288E-4	0.0
16	9.944212965264864E-4	0.0	0.013258950620353153	3.314737655088288E-4	0.0
17	9.944212965264864E-4	0.0	0.013590424385861982	6.629475310176576E-4	0.0
18	9.944212965264864E-4	0.0	0.014253371916879639	6.629475310176576E-4	0.0
19	9.944212965264864E-4	0.0	0.014584845682388468	6.629475310176576E-4	0.0
20	9.944212965264864E-4	0.0	0.014584845682388468	6.629475310176576E-4	0.0
21	9.944212965264864E-4	0.0	0.014916319447897296	6.629475310176576E-4	0.0
22	9.944212965264864E-4	0.0	0.015247793213406125	9.944212965264864E-4	0.0
23	9.944212965264864E-4	0.0	0.015579266978914954	9.944212965264864E-4	0.0
24	9.944212965264864E-4	0.0	0.015579266978914954	9.944212965264864E-4	0.0
25	9.944212965264864E-4	0.0	0.015579266978914954	9.944212965264864E-4	0.0
26	9.944212965264864E-4	0.0	0.015579266978914954	0.0013258950620353151	0.0
27	9.944212965264864E-4	0.0	0.015579266978914954	0.001657368827544144	0.0
28	9.944212965264864E-4	0.0	0.015579266978914954	0.005635054013650089	0.0
29	9.944212965264864E-4	0.0	0.015579266978914954	0.01889400463400324	0.0
30	9.944212965264864E-4	0.0	0.015579266978914954	0.03646211420597117	0.0
31	9.944212965264864E-4	0.0	0.015579266978914954	0.09181823304594558	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCTT	35	8.8541757E-4	26.42857	32
CATACCC	35	8.8541757E-4	26.42857	30
GTATCAA	405	0.0	25.123457	1
GGCCGTG	50	2.6957053E-4	22.199999	11
ACGGGAC	50	2.6957053E-4	22.199999	16
GGTATCA	155	0.0	21.48387	1
TGTATCG	45	0.0038193676	20.555555	5
TGGCCGT	45	0.0038193676	20.555555	10
GTATCGC	45	0.0038193676	20.555555	6
GTTTAGG	55	5.1310175E-4	20.181818	1
CCTATAG	65	6.880584E-5	19.923077	2
ATCAACG	570	0.0	18.82456	3
TCAACGC	570	0.0	18.82456	4
AACGCAG	585	0.0	18.658121	6
CAACGCA	590	0.0	18.5	5
GCGGCCG	80	1.6102385E-5	18.5	12
GGACAGT	70	1.2154186E-4	18.5	6
TATACAC	80	1.6102385E-5	18.5	3
AGTACCG	70	1.2154186E-4	18.5	5
TAGTACC	60	9.215304E-4	18.5	4
>>END_MODULE