FastQCFastQC Report
Fri 10 Feb 2017
ERR1632931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497108
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60951.2260917144765322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.8796881160633102No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA29130.5859893624725412No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27550.554205524755184No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG26630.5356984800083684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22830.4592563386628258No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20550.4133910538555002No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18570.3735606749438754No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17080.34358730899522844No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC15320.30818252774045074No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15040.3025499489044634No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14270.2870603571054982No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT14220.2860545394562148No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13810.2778068347320904No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13160.2647312052914055No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12610.2536672111492875No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12550.25246022997014733No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12400.24944277702229697No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11860.23857994641003563No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11830.23797645582046556No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA11710.23556249346218527No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC11380.2289240969769145No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11310.22751595226791765No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11180.22490082637978062No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.22490082637978062No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT10950.22027406519307674No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10830.21786010283479645No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10350.20820425340167528No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10340.2080030898718186No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC10090.20297400162540133No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10070.20257167456568795No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9800.1971402592595573No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9680.194726296901277No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT9660.1943239698415636No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9360.18828906394586287No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT8830.17762739686345824No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8560.1721959815573276No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8540.17179365449761422No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8430.1695808556691906No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG8430.1695808556691906No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8430.1695808556691906No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8320.167368056840767No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8120.16334478624363316No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8040.16173547800477964No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7970.1603273332957828No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7760.15610289916879228No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA7550.15187846504180177No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7510.151073810922375No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT7300.1468493767953845No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7210.1450389050266743No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7150.14383192384753415No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7100.1428261061982507No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA7000.14081447089968377No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6850.1377970179518334No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6740.13558421912340982No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6660.13397491088455626No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6560.13196327558598936No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6550.13176211205613267No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6530.1313597849964193No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6400.1287446591082823No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG6270.1261295332201453No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6150.12371557086186503No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6130.12331324380215165No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6130.12331324380215165No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.12291091674243827No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5750.11566902966759739No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC5490.1104387778913234No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA5390.1084271425927565No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5360.10782365200318643No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC5340.10742132494347305No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5240.10540968964490613No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5190.10440387199562268No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5090.10239223669705577No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT4980.10017943786863218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCG352.3856017E-531.71428732
TTAAGAC352.3856017E-531.71428729
ACTCGGT352.3856017E-531.71428734
CCAGTTA352.3856017E-531.71428725
GACTCGG352.3856017E-531.71428733
ATCTCGT303.596567E-430.83333237
GACACTC303.596567E-430.83333226
ACACTCG303.596567E-430.83333227
TCCCATG303.596567E-430.8333322
CGGCTGT250.005493380629.636
GTGAGAC250.005493380629.63
GGTATCA8950.029.1452521
CGTTACG405.9334536E-527.7516
TCAAATT405.9334536E-527.7514
GCGACGC358.862286E-426.42857223
GTTAAGA509.077543E-625.90000228
GTATCAA29450.025.6926981
GGACGTA659.3694325E-825.61538527
CCCAGTT451.3217365E-424.66666824
TTCAAAT451.3217365E-424.66666813
Produced by FastQC (version 0.11.5)