FastQCFastQC Report
Fri 10 Feb 2017
ERR1632930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546707
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18020.3296098275676002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.23303158730361967No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG11560.2114478139112907No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC11550.2112649005774574No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT11350.20760663390079148No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC10430.1907786071881282No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC8540.15620798709363518No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8460.1547446804229688No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC8050.14724523373580364No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7910.14468444706213748No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA7710.14102618038547154No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC7450.13627043370580585No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA7160.13096594702464026No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT7140.13060012035697366No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA6870.12566146034347464No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG6800.12438106700664159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6680.12218610700064203No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT6010.10993091363381118No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC5940.1086505202969781No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA5920.10828469362931151No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC5650.10334603361581249No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG5630.1029802069481459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG251.232779E-437.05
TACGGCC250.005493853729.619
CCGTTTA250.005493853729.627
GGTATCA3400.028.8382361
GTTACGG358.8634243E-426.4285717
TTTGCCG509.079706E-625.9000029
CCGTCCG901.4188117E-1024.66666828
TTAACGG601.3352637E-624.66666635
TTCGCCG1051.8189894E-1224.66666624
GTATCAA10600.024.4339621
CGCTCTC1054.0017767E-1122.9047629
AACGGCC652.6785747E-622.7692337
CCGAGTT652.6785747E-622.7692313
TCGCCTG502.6995913E-422.235
CGAGTCG1201.0913936E-1121.58333221
GAGTCGC1201.0913936E-1121.58333222
TACTAGT603.7216876E-521.5833327
TCCGCCG957.1249815E-921.42105331
CGCCGTC1301.8189894E-1221.34615326
TAACGGC705.0938525E-621.14285736
Produced by FastQC (version 0.11.5)