FastQCFastQC Report
Fri 10 Feb 2017
ERR1632929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456359
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9040.1980896618670827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.13476232527461932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2500.026.641
TCGGTGT358.86117E-426.4285733
GTCCTAG652.676963E-622.7692341
GTATCAA5750.020.5913031
AGTAGTC500.007028541518.522
CTATACA752.0638271E-417.2666664
GAATTAT904.436512E-516.4444451
TAGACGG803.376978E-416.18754
TAATATA1059.3183535E-615.8571424
GACGGGT700.002589283715.8571426
CGAACTA1302.5787813E-715.65384824
AGGTCGC957.0447735E-515.57894835
AAAGCGA1455.320362E-815.31034520
ATAGACG855.355354E-415.2352943
GTATAGA855.355354E-415.2352941
TAGGACC855.355354E-415.2352944
TCAATAC750.00409936614.83
GTCTTAA750.00409936614.81
GCTCGGA1001.0910706E-414.811
TAACCGT1152.2039667E-514.4782617
Produced by FastQC (version 0.11.5)