##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632929.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	456359
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.663072274240236	33.0	33.0	33.0	27.0	33.0
2	31.183412620327417	33.0	33.0	33.0	27.0	33.0
3	31.61277415368164	33.0	33.0	33.0	27.0	33.0
4	31.766127106072194	33.0	33.0	33.0	27.0	33.0
5	31.859737618848317	33.0	33.0	33.0	33.0	33.0
6	34.724488834448316	37.0	37.0	37.0	27.0	37.0
7	34.927638109470834	37.0	37.0	37.0	27.0	37.0
8	35.082086690522154	37.0	37.0	37.0	33.0	37.0
9	35.1795954500733	37.0	37.0	37.0	33.0	37.0
10	35.21448245788951	37.0	37.0	37.0	33.0	37.0
11	35.24553038287839	37.0	37.0	37.0	33.0	37.0
12	35.194912338750854	37.0	37.0	37.0	33.0	37.0
13	35.21993649736282	37.0	37.0	37.0	33.0	37.0
14	35.17487109928806	37.0	37.0	37.0	33.0	37.0
15	35.202616799493384	37.0	37.0	37.0	33.0	37.0
16	35.21180255018527	37.0	37.0	37.0	33.0	37.0
17	35.18798358310015	37.0	37.0	37.0	33.0	37.0
18	35.19061309188599	37.0	37.0	37.0	33.0	37.0
19	35.18929614623575	37.0	37.0	37.0	33.0	37.0
20	35.189502124423974	37.0	37.0	37.0	33.0	37.0
21	35.20249189782605	37.0	37.0	37.0	33.0	37.0
22	35.20918838020067	37.0	37.0	37.0	33.0	37.0
23	35.19780698967261	37.0	37.0	37.0	33.0	37.0
24	35.188706698016254	37.0	37.0	37.0	33.0	37.0
25	35.18313433064758	37.0	37.0	37.0	33.0	37.0
26	35.14445425640778	37.0	37.0	37.0	33.0	37.0
27	35.087047697098114	37.0	37.0	37.0	33.0	37.0
28	35.05392465142574	37.0	37.0	37.0	27.0	37.0
29	35.090420042115966	37.0	37.0	37.0	33.0	37.0
30	35.0597292920705	37.0	37.0	37.0	27.0	37.0
31	35.038395210787996	37.0	37.0	37.0	27.0	37.0
32	35.068244518022	37.0	37.0	37.0	27.0	37.0
33	35.05137183664615	37.0	37.0	37.0	27.0	37.0
34	34.98760405733206	37.0	37.0	37.0	27.0	37.0
35	34.98100399027958	37.0	37.0	37.0	27.0	37.0
36	34.98049123606634	37.0	37.0	37.0	27.0	37.0
37	35.00727716556483	37.0	37.0	37.0	27.0	37.0
38	34.87008911843527	37.0	37.0	37.0	27.0	37.0
39	34.94351376876538	37.0	37.0	37.0	27.0	37.0
40	34.979075683836626	37.0	37.0	37.0	27.0	37.0
41	35.01337543469067	37.0	37.0	37.0	27.0	37.0
42	34.91644516707242	37.0	37.0	37.0	27.0	37.0
43	34.70780898371677	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	19.0
17	19.0
18	16.0
19	19.0
20	44.0
21	102.0
22	286.0
23	650.0
24	1247.0
25	2398.0
26	3976.0
27	5890.0
28	8278.0
29	11078.0
30	14430.0
31	18558.0
32	23382.0
33	30189.0
34	43087.0
35	77163.0
36	215518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.96007748285889	20.295644437821977	13.070411671512996	25.673866407806134
2	16.365186180178323	22.53664330055943	37.02392195617924	24.07424856308301
3	17.986716598116832	26.14235722315107	29.922714354269335	25.948211824462756
4	12.928856448541609	17.50244873005682	38.55999333857774	31.00870148282383
5	14.116737042547644	36.78485578239938	35.39581776627611	13.702589408776861
6	30.566067503873047	39.06222951667437	17.21123063202435	13.160472347428232
7	27.2785241443688	31.617432766747232	23.58099654000469	17.523046548879282
8	24.96258428123473	35.730641885007195	20.448375073133214	18.85839876062486
9	26.04002550623522	15.2347165279966	19.980322509252584	38.74493545651559
10	14.789891291724278	28.140126523197743	34.26885412580884	22.801128059269125
11	34.76714604072671	23.151729230715294	23.847453430303776	18.233671298254226
12	22.877383814058668	25.33049638552105	30.379591505810115	21.412528294610166
13	28.2144101463979	21.797093954540177	25.804246218437676	24.184249680624244
14	22.005920777282796	20.513893667047213	27.130614275164948	30.34957128050504
15	23.93773323195116	28.682681836010687	23.66470256968746	23.714882362350693
16	23.489401983964378	27.982136870314818	24.92905804421519	23.59940310150561
17	22.86511277305805	27.420955870268806	26.186620621046153	23.527310735626997
18	23.344779000742836	27.341413229496954	27.03989622205325	22.27391154770696
19	24.71519132963303	26.45614527159539	26.628597222800472	22.200066175971113
20	24.30630271343394	26.348116285643542	26.76007266209278	22.585508338829737
21	23.320894295938068	26.900751382135557	26.854077601186788	22.92427672073959
22	23.21812432755791	26.675709255213548	26.7044147261257	23.401751691102838
23	23.178243444305906	26.580608687458778	27.160415374737873	23.080732493497443
24	23.249678432988063	27.004836104908637	26.05536430748599	23.69012115461731
25	23.504740785215148	26.674394500820625	26.813977592202633	23.006887121761597
26	23.31147188945545	26.90250438799279	26.796447533630325	22.98957618892144
27	23.470995422463456	26.977664514121557	26.14805449218707	23.403285571227915
28	23.208044543878835	26.683816907303243	26.988182549264945	23.11995599955298
29	24.205285750911017	26.52911414040262	26.364769841287234	22.90083026739913
30	23.64936376843669	26.49712178350816	26.683378655838936	23.170135792216215
31	23.61298889689915	26.42086602871862	26.76708468552171	23.199060388860524
32	22.86949528770113	26.535468786635086	26.80302130559494	23.792014620068848
33	22.810769591483897	26.545986821778467	27.37143345480203	23.271810131935602
34	23.49049761262515	26.074866497647687	27.024995672266787	23.409640217460375
35	23.465736404891764	26.834137159560782	26.63758137781878	23.062545057728673
36	24.041598828992086	26.57578792135139	26.290267092354924	23.0923461573016
37	23.29832434552622	26.153313509758764	26.76007266209278	23.788289482622236
38	23.487429852374994	26.5733775382977	26.67702400960647	23.262168599720834
39	23.04764450794221	26.441244721808925	26.9404131396554	23.57069763059346
40	23.068899703961137	26.831726776507093	27.176630678917256	22.922742840614514
41	23.18613197066345	26.104010220024147	27.056111526232634	23.65374628307977
42	22.89732425568467	26.833698908096476	26.83567103968586	23.433305796532995
43	23.44776809485515	25.819146768224137	26.959257952620636	23.77382718430008
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	10.5
2	15.0
3	58.5
4	102.0
5	102.0
6	136.0
7	170.0
8	148.0
9	126.0
10	183.5
11	241.0
12	241.0
13	380.0
14	519.0
15	900.0
16	1281.0
17	1592.5
18	1904.0
19	1904.0
20	1948.5
21	1993.0
22	2469.0
23	2945.0
24	4009.5
25	5074.0
26	5074.0
27	6799.0
28	8524.0
29	10548.0
30	12572.0
31	15074.0
32	17576.0
33	17576.0
34	20598.0
35	23620.0
36	26139.5
37	28659.0
38	30608.5
39	32558.0
40	32558.0
41	34265.0
42	35972.0
43	36402.0
44	36832.0
45	36101.5
46	35371.0
47	35371.0
48	34611.0
49	33851.0
50	33489.0
51	33127.0
52	31622.5
53	30118.0
54	30118.0
55	28950.5
56	27783.0
57	24751.5
58	21720.0
59	20378.0
60	19036.0
61	19036.0
62	16926.5
63	14817.0
64	12636.5
65	10456.0
66	8869.5
67	7283.0
68	7283.0
69	6057.5
70	4832.0
71	3967.0
72	3102.0
73	2468.5
74	1835.0
75	1835.0
76	1448.0
77	1061.0
78	790.5
79	520.0
80	431.0
81	342.0
82	342.0
83	268.5
84	195.0
85	161.0
86	127.0
87	97.0
88	67.0
89	67.0
90	45.5
91	24.0
92	13.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	456359.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.9498276255046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.39115384129535	61.82945974818068
2	6.824433418349515	9.547358825105634
3	1.856312926946104	3.8954630777662773
4	0.8119447309802191	2.2718157589401216
5	0.49253794987473765	1.722647234637866
6	0.30811746022951275	1.2931657938877612
7	0.21349646364503994	1.045382858143775
8	0.1678047662721704	0.9390331580381127
9	0.11588448001911902	0.7295489461627747
>10	0.7227856935999356	9.571113380670809
>50	0.06589243638037494	3.195099336294887
>100	0.02900528278231387	3.6249204503926316
>500	6.305496257024753E-4	0.33499143177869484
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	904	0.1980896618670827	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	615	0.13476232527461932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0013147543929231153	0.0	0.0
7	0.0	0.0	0.002410383053692378	0.0	0.0
8	0.0	0.0	0.002410383053692378	0.0	0.0
9	0.0	0.0	0.003067760250153936	4.382514643077051E-4	0.0
10	0.0	0.0	0.0035060117144616407	4.382514643077051E-4	0.0
11	0.0	0.0	0.004820766107384756	4.382514643077051E-4	0.0
12	0.0	0.0	0.005039891839538609	4.382514643077051E-4	0.0
13	0.0	0.0	0.005259017571692461	4.382514643077051E-4	0.0
14	0.0	0.0	0.005478143303846314	4.382514643077051E-4	0.0
15	0.0	0.0	0.006135520500307872	4.382514643077051E-4	0.0
16	0.0	0.0	0.0070120234289232815	4.382514643077051E-4	0.0
17	2.1912573215385255E-4	0.0	0.007450274893230987	4.382514643077051E-4	0.0
18	2.1912573215385255E-4	0.0	0.007669400625384839	4.382514643077051E-4	0.0
19	2.1912573215385255E-4	0.0	0.007888526357538693	4.382514643077051E-4	0.0
20	2.1912573215385255E-4	0.0	0.007888526357538693	8.765029286154102E-4	0.0
21	2.1912573215385255E-4	0.0	0.008326777821846397	0.0010956286607692628	0.0
22	2.1912573215385255E-4	0.0	0.008326777821846397	0.0019721315893846732	0.0
23	2.1912573215385255E-4	0.0	0.008545903554000249	0.0019721315893846732	0.0
24	2.1912573215385255E-4	2.1912573215385255E-4	0.008545903554000249	0.0019721315893846732	0.0
25	2.1912573215385255E-4	2.1912573215385255E-4	0.008545903554000249	0.0019721315893846732	0.0
26	2.1912573215385255E-4	2.1912573215385255E-4	0.008545903554000249	0.0019721315893846732	0.0
27	2.1912573215385255E-4	2.1912573215385255E-4	0.008545903554000249	0.0028486345180000834	0.0
28	2.1912573215385255E-4	2.1912573215385255E-4	0.008545903554000249	0.008545903554000249	0.0
29	2.1912573215385255E-4	2.1912573215385255E-4	0.008545903554000249	0.01796831003661591	0.0
30	2.1912573215385255E-4	2.1912573215385255E-4	0.008545903554000249	0.03571749434107797	0.0
31	2.1912573215385255E-4	2.1912573215385255E-4	0.008545903554000249	0.09619619641554128	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	250	0.0	26.64	1
TCGGTGT	35	8.86117E-4	26.42857	33
GTCCTAG	65	2.676963E-6	22.769234	1
GTATCAA	575	0.0	20.591303	1
AGTAGTC	50	0.0070285415	18.5	22
CTATACA	75	2.0638271E-4	17.266666	4
GAATTAT	90	4.436512E-5	16.444445	1
TAGACGG	80	3.376978E-4	16.1875	4
TAATATA	105	9.3183535E-6	15.857142	4
GACGGGT	70	0.0025892837	15.857142	6
CGAACTA	130	2.5787813E-7	15.653848	24
AGGTCGC	95	7.0447735E-5	15.578948	35
AAAGCGA	145	5.320362E-8	15.310345	20
ATAGACG	85	5.355354E-4	15.235294	3
GTATAGA	85	5.355354E-4	15.235294	1
TAGGACC	85	5.355354E-4	15.235294	4
TCAATAC	75	0.004099366	14.8	3
GTCTTAA	75	0.004099366	14.8	1
GCTCGGA	100	1.0910706E-4	14.8	11
TAACCGT	115	2.2039667E-5	14.478261	7
>>END_MODULE