FastQCFastQC Report
Fri 10 Feb 2017
ERR1632928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332341
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11880.3574641708365805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.2708061900277125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4560.137208469614041No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3590.10802158024438754No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3410.10260545644383329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG355.6152567E-737.021
AACGGCC352.383239E-531.71428537
GGTATCA2350.028.3404271
TTAACGG405.92762E-527.75000235
TCGCCGT509.0657195E-625.89999825
TCCGCCG509.0657195E-625.89999831
CGCCGTC451.3204488E-424.66666626
GAGTTCC551.8973866E-523.54545415
GTATCAA6500.023.338461
CGTGCAA400.001928314323.12500211
CTAACCG400.001928314323.1250026
TAACCGT400.001928314323.1250027
GGGTAAG502.6965048E-422.1999991
TAACGGC502.6965048E-422.19999936
CCGTCCG502.6965048E-422.19999928
TGCCGAG502.6965048E-422.19999911
GAGTCGC603.716034E-521.58333422
TCGAGCT603.716034E-521.58333427
AGTCGCC603.716034E-521.58333423
CACTAAC806.929513E-720.812532